The Potential of Phenothiazines against Endodontic Pathogens: A Focus on Enterococcus-Candida Dual-Species Biofilm
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of Phenothiazines against Planktonic E. faecalis and C. albicans
2.2. Effect of Phenothiazines on Dual-Species Biofilms
2.2.1. Colony-Forming-Unit Counts and Crystal Violet Staining Assay
2.2.2. Confocal Laser Microscopy
2.2.3. Biochemical Composition
2.3. Discussion
- In the biofilm susceptibility assay, the concentration used was 10× higher than the MICs of promethazine (1280 µg/mL) and chlorpromazine (500 µg/mL) obtained against planktonic growth;
- The presence of E.faecalis in the dual-species biofilm may modulate the susceptibility of C. albicans to the phenothiazines, as antagonistic interactions between these pathogens have already been suggested [38].
3. Material and Methods
3.1. Strains and Media
3.2. Antimicrobial Susceptibility Assays
3.3. Chequerboard Interaction Assay between Phenothiazines and Chlorhexidine
3.4. Biofilm Formation
3.5. Biofilm Susceptibility Assays
3.6. Colony Forming Units Assay
3.7. Crystal Violet Assay
3.8. Confocal Laser Scanning Microscopy
3.9. Biochemical Composition of Biofilms
3.10. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | NaOCl | CHX | PMZ | CPZ | PMZ + CHX | CPZ + CHX | ||
---|---|---|---|---|---|---|---|---|
MIC | FICI | MIC | FICI | |||||
E. faecalis ATCC 29212 | 2500 | 4 | 64 | 32 | 8 + 1 | 0.562 | 0.25 + 1 | 0.507 |
C. albicans ATCC 10231 | 625 | 4 | 128 | 50 | 8 + 2 | 0.531 | 12.5 + 2 | 1 |
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Fiallos, N.d.M.; Ribeiro Aguiar, A.L.; da Silva, B.N.; Pergentino, M.L.M.; Rocha, M.F.G.; Sidrim, J.J.C.; Maia, D.C.B.d.S.C.; Cordeiro, R.d.A. The Potential of Phenothiazines against Endodontic Pathogens: A Focus on Enterococcus-Candida Dual-Species Biofilm. Antibiotics 2022, 11, 1562. https://doi.org/10.3390/antibiotics11111562
Fiallos NdM, Ribeiro Aguiar AL, da Silva BN, Pergentino MLM, Rocha MFG, Sidrim JJC, Maia DCBdSC, Cordeiro RdA. The Potential of Phenothiazines against Endodontic Pathogens: A Focus on Enterococcus-Candida Dual-Species Biofilm. Antibiotics. 2022; 11(11):1562. https://doi.org/10.3390/antibiotics11111562
Chicago/Turabian StyleFiallos, Nicole de Mello, Ana Luiza Ribeiro Aguiar, Bruno Nascimento da Silva, Mariana Lara Mendes Pergentino, Marcos Fábio Gadelha Rocha, José Júlio Costa Sidrim, Débora Castelo Branco de Souza Collares Maia, and Rossana de Aguiar Cordeiro. 2022. "The Potential of Phenothiazines against Endodontic Pathogens: A Focus on Enterococcus-Candida Dual-Species Biofilm" Antibiotics 11, no. 11: 1562. https://doi.org/10.3390/antibiotics11111562