Corrigendum: Single-cell RNA sequencing reveals the role of phosphorylation-related genes in hepatocellular carcinoma stem cells
Fuwen Yao1,2† 
Yongqiang Zhan1† 
Changzheng Li3† 
Ying Lu2 
Jiao Chen2 
Jing Deng2 Zijing Wu2 Qi Li2 Yi’an Song2 Binhua Chen1 Jinjun Chen1 Kuifeng Tian1 
Zuhui Pu4* Yong Ni1* 
Lisha Mou1,2*- 1Department of Hepatopancreatobiliary Surgery, Shenzhen Institute of Translational Medicine, Health Science Center, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China
- 2Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Institute of Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, China
- 3Key Laboratory of Stem Cells and Tissue Engineering, Zhongshan School of Medicine, Sun Yat-sen University, Ministry of Education, Guangzhou, China
- 4Imaging Department, Shenzhen Institute of Translational Medicine, Health Science Center, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China
A Corrigendum on
Single-cell RNA sequencing reveals the role of phosphorylation-related genes in hepatocellular carcinoma stem cells
by Yao F, Zhan Y, Li C, Lu Y, Chen J, Deng J, Wu Z, Li Q, Song Y, Chen B, Chen J, Tian K, Pu Z, Ni Y and Mou L (2022). Front. Cell Dev. Biol. 9:734287. doi: 10.3389/fcell.2021.734287
In the published article, there was an error in Figure 7C as published. The red label and black label were reversed. The corrected Figure 7C and its caption is as follows: “(C) Hep3B and Huh7 cells were treated with gambogenic acid (EZH2 inhibitor, 2 μM) or alisertib (AURKA inhibitor, 10 μM) for 0, 12, 24, 32, 48, and 60 h, and cell viability was determined by Calcein-AM/PI staining assays.”
FIGURE 7. Gambogenic acid (EZH2 inhibitor) and alisertib (AURKA inhibitor) inhibit HCC cell proliferation, migration, and invasion. (A) Hep3B and Huh7 cells were treated with gambogenic acid (EZH2 inhibitor) or alisertib (AURKA inhibitor) at different concentrations (0–100 μM) for 48 h, and cell viability was determined by Calcein–AM/PI staining assays. (B) Hep3B and Huh7 cells were treated with DMSO (solvents of gambogenic acid and alisertib) at different concentrations (0–100 μM) for 48 h, and cell viability was determined by Calcein–AM/PI staining assays. (C) Hep3B and Huh7 cells were treated with gambogenic acid (EZH2 inhibitor, 2 μM) or alisertib (AURKA inhibitor, 10 μM) for 0, 12, 24, 32, 48, and 60 h, and cell viability was determined by Calcein–AM/PI staining assays. (D) Colony formation assays were conducted to analyze Hep3B and Huh7 cell proliferation with gambogenic acid (2 μM) or alisertib (10 μM) treatment. (E, F) Wound healing assays (E) and Transwell assays (F) were performed to detect the cell migratory abilities of Hep3B and Huh7 cells treated with gambogenic acid (2 μM) or alisertib (10 μM). (G) Transwell assays were performed to detect the cell invasion abilities of Hep3B and Huh7 cells treated with gambogenic acid (2 μM) or alisertib (10 μM). Data are expressed as the means ± s.d. Differences were considered statistically significant if p < 0.05. ns, no significance, *p < 0.05, **p < 0.01, ***p < 0.001. (H, I) Expression of AURKA (H) and EZH2 (I) in TCGA–LIHC based on TP3 mutation status.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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Keywords: AURKA, EZH2, tyrosine kinase inhibitors, TKI, protein kinases, cell cycle, single-cell RNA sequencing, hepatocellular carcinoma
Citation: Yao F, Zhan Y, Li C, Lu Y, Chen J, Deng J, Wu Z, Li Q, Song Y, Chen B, Chen J, Tian K, Pu Z, Ni Y and Mou L (2023) Corrigendum: Single-cell RNA sequencing reveals the role of phosphorylation-related genes in hepatocellular carcinoma stem cells. Front. Cell Dev. Biol. 11:1045260. doi: 10.3389/fcell.2023.1045260
Received: 15 September 2022; Accepted: 11 October 2023;
Published: 23 October 2023.
Edited and reviewed by:
Lei Dong, Beijing Institute of Technology, ChinaCopyright © 2023 Yao, Zhan, Li, Lu, Chen, Deng, Wu, Li, Song, Chen, Chen, Tian, Pu, Ni and Mou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Zuhui Pu, pupeter190@163.com; Yong Ni, szniyong@sina.com; Lisha Mou, lishamou@gmail.com
†These authors have contributed equally to this work