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IDENTIFICATION AND QUANTIFICATION OF β- SITOSTEROL IN LEAF OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA BY HPTLC

Yıl 2021, Cilt: 45 Sayı: 2, 253 - 263, 31.05.2021

Shripad Bairagi Prashant Ghule Ritu Gilhotra

https://doi.org/10.33483/jfpau.869819
Cited By: 1

Öz

Objective: The distinguishing proof and measurement of biomarker β-sitosterol present in a leaf of Calotropis procera and Calotropis gigantea belonging to family Asclepidiaceae was carried by utilizing high performance thin-layer chromatographic (HPTLC) characterization method. In the present research work the Exterinsic Standard Method is used for quantitative assessment.
Material and Method: It assures the accuracy of quantitative investigation and particularly benifit for HPTLC analysis. Aluminum-maintained silica gel 60F254 HPTLC plates of 250μm thickness was used for chromatographic analysis. Toluene: Ethyl acetate: Glacial acetic acid acidic 8:2:0.20 (v/v/v) solvent system was used as mobile phase. Anisaldehyde sulphuric acid is used for derivatisation and was evaluated at 510 nm.
Result and Discussion: β-sitosterol quantification in foundations of plant was observed as 11.63 µg and 10.23 µg for Calotropi sprocera and Calotropis gigantea respectively. The appropriateness and particularity of the established techniques was demonstrated by qualitative and quantative analysis. This established method for estimation of β-sitosterol in the leaf ofCalotropis procera and Calotropis gigantea was proved to be responsive, particular and replicable.

Anahtar Kelimeler

Calotropis procera Calotropis gigantea HPTLC identification quantification

Kaynakça

  • 1. Aparna S., Aruna S, HPTLC Method for Identification and Quantification of β- sitosterol in the Roots of Achyranthes aspera Linn., International Journal of Pharmaceutical Science, 2015;5(4): 1117-1122.
  • 2. P. P., J. Thomas, S. Mathew and B. P. Skaria: Medicinal Plants, Tropical Horticulture, 2001; 2:449-632.
  • 3. Khairnar AK, Bhamare SR, Bhamare HP. Calotropi sprocera: An ethnopharmacological update, Advance Research in Pharmaceuticals and Biologicals, 2012;2(2):142-56.
  • 4. Rahman M, Wilcock C. A taxonomic revision of Calotropis (Asclepiadaceae). Nordic Journal of Botany.,1991;11(3):301-8.
  • 5. Chatterjee A, Chandra PS. The treatise of Indian medicinal plants. CSIR, New Delhi. 1995; 4:130.
  • 6. Shripad MB, Prashant GJ, RituGilhotra, Pharmacology of Natural Products: An recent approach on Calotropis gigantean and Calotropis procera, Ars. Pharmaceutica.2018; 59(1): 37-44.
  • 7. Palejkar Carol J., PalejkarJignesh H., Patel Mayuree A., Patel Anar J. A Comprehensive Review On Plant Calotropis Gigantea. International Journal of Institutional Pharmacy and Life Sciences, 2012; 2(2):463 – 470.
  • 8. Abhishek, D., Mohit, C., Ashish, G., &Ameeta, A. Medicinal utility of Calotropis procera (Ait.) R. Br. as used by natives of village Sanwer of Indore District, Madhya Pradesh. International Journal of Pharmacy & Life Sciences, 2010; 1(3): 188-190.
  • 9. Esmail, A. & Al-Snafi. The constituents and pharmacological properties of Calotropis procera-an overview. International Journal of Pharmacy Review and Research, 2015; 5(3): 259-275.
  • 10. Kitagawa I, R.S. Zhang, J.D. Park, N.I, Baek, Y. Takeda, N. Yoshikawa and H. Shibuya, Indonesian plants, I. chemical structures of calotroposides A and B two new oxypregane-oligoglycosides from the root of Calotropisgigantea (Asclepiadaceae). Chemical and Pharmaceutical Bulletin,1992;140:2007-2013
  • 11. Pari, K., P.J. Rao, C. Devakumar and J.N. Rstogi, 1998. A novel insect antifeedantnonprotein amino acid from Calotropics gigantea, Journal of Natural Product, 1998; 61: 102-104.
  • 12. Sen, S., N.P. Sahu and S.B. Mahato. Flavonol glycosides from Calotropics gigantea, phytochemistry, 1992; 31: 2919-21
  • 13. Khandelwal K, Practical Pharmacognosy, Techniques and experiments, Nirali Publication, 2008, 19th edn, 149-155
  • 14. Mukherjee PK, Quality control of Herbal Drugs- An approach to evaluation of Botanicals, 2002: 428-456.
  • 15. Shah SA, Ravishankara MN, Nirmal A, Shishoo CJ, Rathod IS, Suhagia B N. Estimation of individual sennosides in plant materials and marketed formulations by HPTLC method. Journal of Pharmacy and Pharmacology, 2000; 52: 445-9.
  • 16. Stahl E, Thin Layer Chromatography- A Laboratory Handbook, Spring International Student Edition, 1969; 855 – 858, 883, 889, 904.
  • 17. Shaikh BT, Hatcher J. Complementary and alternative medicine in Pakistan: prospects and limitations. Evidence Based Complementary and Alternative Medicine 2005; 2(2): 139-42.
  • 18. Agrawal S, Paridhavi M. Herbal drug technology. Hyderabad: Universities Press Private Limited; 2007.
  • 19. Chawla R, Thakur P, Chowdhry A, Jaiswal S, Sharma A, Goel R, et al. Evidence based herbal drug standardization approach in coping with challenges of holistic management of diabetes: a dreadful lifestyle disorder of 21st century. Journal of Diabetes and Metabolic Disorders 2013; 12: 35.

HPTLC İLE CALOTROPIS GIGANTEA VE CALOTROPIS PROCERA YAPRAĞINDAKİ β-SİTOSTEROLÜN TANIMLANMASI VE MİKTAR TAYİNİ

Yıl 2021, Cilt: 45 Sayı: 2, 253 - 263, 31.05.2021

Shripad Bairagi Prashant Ghule Ritu Gilhotra

https://doi.org/10.33483/jfpau.869819
Cited By: 1

Öz

Amaç: Asclepidiaceae familyasına ait Calotropis procerave Calotropis gigantean yaprağında bulunan biyomarker β-sitosterol'ün ayırt edici kanıtı ve ölçümü, yüksek performanslı ince tabaka kromatografik (HPTLC) karakterizasyon yöntemi kullanılarak gerçekleştirildi. Bu çalışmada, kantitatif değerlendirme için dış standart yöntemi kullanılmıştır.
Gereç ve Yöntem: Yöntem, kantitatif araştırmanın doğruluğunu sağlamaktadır ve özellikle HPTLC analizi için uygundur. Kromatografik analiz için, 250 μm kalınlığında silika jel 60F254 kaplı alüminyum HPTLC plakları kullanıldı. Mobil faz olarak; toluen: etil asetat: glasiyal asetik asit 8: 2: 0.20 (v / v / v) çözücü sistemi kullanıldı. Türevlendirme için anisaldehit sülfürik asit kullanıldı ve 510 nmdalga boyunda değerlendirildi.
Sonuç ve Tartışma: Bitki yapısındaki β-sitosterol miktarının Calotropis procera ve Calotropis gigantea için sırasıyla 11,63 µg ve 10,23 µg olduğu görülmüştür. Kullanılan yöntemlerin uygunluğu ve seçiciliği, nitel ve nicel analizlerle gösterilmiştir. Belirlenen bu yöntemin;Calotropis procera ve Calotropis gigantea yaprağındaki β-sitosterol miktarı tayini için, hassas, seçici ve tekrar edilebilir olduğu kanıtlanmıştır.

Anahtar Kelimeler

Calotropis procera Calotropis gigantea HPTLC miktar tayini teşhis

Kaynakça

  • 1. Aparna S., Aruna S, HPTLC Method for Identification and Quantification of β- sitosterol in the Roots of Achyranthes aspera Linn., International Journal of Pharmaceutical Science, 2015;5(4): 1117-1122.
  • 2. P. P., J. Thomas, S. Mathew and B. P. Skaria: Medicinal Plants, Tropical Horticulture, 2001; 2:449-632.
  • 3. Khairnar AK, Bhamare SR, Bhamare HP. Calotropi sprocera: An ethnopharmacological update, Advance Research in Pharmaceuticals and Biologicals, 2012;2(2):142-56.
  • 4. Rahman M, Wilcock C. A taxonomic revision of Calotropis (Asclepiadaceae). Nordic Journal of Botany.,1991;11(3):301-8.
  • 5. Chatterjee A, Chandra PS. The treatise of Indian medicinal plants. CSIR, New Delhi. 1995; 4:130.
  • 6. Shripad MB, Prashant GJ, RituGilhotra, Pharmacology of Natural Products: An recent approach on Calotropis gigantean and Calotropis procera, Ars. Pharmaceutica.2018; 59(1): 37-44.
  • 7. Palejkar Carol J., PalejkarJignesh H., Patel Mayuree A., Patel Anar J. A Comprehensive Review On Plant Calotropis Gigantea. International Journal of Institutional Pharmacy and Life Sciences, 2012; 2(2):463 – 470.
  • 8. Abhishek, D., Mohit, C., Ashish, G., &Ameeta, A. Medicinal utility of Calotropis procera (Ait.) R. Br. as used by natives of village Sanwer of Indore District, Madhya Pradesh. International Journal of Pharmacy & Life Sciences, 2010; 1(3): 188-190.
  • 9. Esmail, A. & Al-Snafi. The constituents and pharmacological properties of Calotropis procera-an overview. International Journal of Pharmacy Review and Research, 2015; 5(3): 259-275.
  • 10. Kitagawa I, R.S. Zhang, J.D. Park, N.I, Baek, Y. Takeda, N. Yoshikawa and H. Shibuya, Indonesian plants, I. chemical structures of calotroposides A and B two new oxypregane-oligoglycosides from the root of Calotropisgigantea (Asclepiadaceae). Chemical and Pharmaceutical Bulletin,1992;140:2007-2013
  • 11. Pari, K., P.J. Rao, C. Devakumar and J.N. Rstogi, 1998. A novel insect antifeedantnonprotein amino acid from Calotropics gigantea, Journal of Natural Product, 1998; 61: 102-104.
  • 12. Sen, S., N.P. Sahu and S.B. Mahato. Flavonol glycosides from Calotropics gigantea, phytochemistry, 1992; 31: 2919-21
  • 13. Khandelwal K, Practical Pharmacognosy, Techniques and experiments, Nirali Publication, 2008, 19th edn, 149-155
  • 14. Mukherjee PK, Quality control of Herbal Drugs- An approach to evaluation of Botanicals, 2002: 428-456.
  • 15. Shah SA, Ravishankara MN, Nirmal A, Shishoo CJ, Rathod IS, Suhagia B N. Estimation of individual sennosides in plant materials and marketed formulations by HPTLC method. Journal of Pharmacy and Pharmacology, 2000; 52: 445-9.
  • 16. Stahl E, Thin Layer Chromatography- A Laboratory Handbook, Spring International Student Edition, 1969; 855 – 858, 883, 889, 904.
  • 17. Shaikh BT, Hatcher J. Complementary and alternative medicine in Pakistan: prospects and limitations. Evidence Based Complementary and Alternative Medicine 2005; 2(2): 139-42.
  • 18. Agrawal S, Paridhavi M. Herbal drug technology. Hyderabad: Universities Press Private Limited; 2007.
  • 19. Chawla R, Thakur P, Chowdhry A, Jaiswal S, Sharma A, Goel R, et al. Evidence based herbal drug standardization approach in coping with challenges of holistic management of diabetes: a dreadful lifestyle disorder of 21st century. Journal of Diabetes and Metabolic Disorders 2013; 12: 35.
Toplam 19 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılık ve İlaç Bilimleri
Bölüm Araştırma Makalesi
Yazarlar

Shripad Bairagi 0000-0002-4313-0580

Prashant Ghule Bu kişi benim 0000-0002-6126-9105

Ritu Gilhotra Bu kişi benim 0000-0003-2736-4886

Yayımlanma Tarihi 31 Mayıs 2021
Gönderilme Tarihi 4 Şubat 2021
Kabul Tarihi 17 Mart 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 45 Sayı: 2

Kaynak Göster

APA Bairagi, S., Ghule, P., & Gilhotra, R. (2021). IDENTIFICATION AND QUANTIFICATION OF β- SITOSTEROL IN LEAF OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA BY HPTLC. Journal of Faculty of Pharmacy of Ankara University, 45(2), 253-263. https://doi.org/10.33483/jfpau.869819
AMA Bairagi S, Ghule P, Gilhotra R. IDENTIFICATION AND QUANTIFICATION OF β- SITOSTEROL IN LEAF OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA BY HPTLC. Ankara Ecz. Fak. Derg. Mayıs 2021;45(2):253-263. doi:10.33483/jfpau.869819
Chicago Bairagi, Shripad, Prashant Ghule, ve Ritu Gilhotra. “IDENTIFICATION AND QUANTIFICATION OF β- SITOSTEROL IN LEAF OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA BY HPTLC”. Journal of Faculty of Pharmacy of Ankara University 45, sy. 2 (Mayıs 2021): 253-63. https://doi.org/10.33483/jfpau.869819.
EndNote Bairagi S, Ghule P, Gilhotra R (01 Mayıs 2021) IDENTIFICATION AND QUANTIFICATION OF β- SITOSTEROL IN LEAF OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA BY HPTLC. Journal of Faculty of Pharmacy of Ankara University 45 2 253–263.
IEEE S. Bairagi, P. Ghule, ve R. Gilhotra, “IDENTIFICATION AND QUANTIFICATION OF β- SITOSTEROL IN LEAF OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA BY HPTLC”, Ankara Ecz. Fak. Derg., c. 45, sy. 2, ss. 253–263, 2021, doi: 10.33483/jfpau.869819.
ISNAD Bairagi, Shripad vd. “IDENTIFICATION AND QUANTIFICATION OF β- SITOSTEROL IN LEAF OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA BY HPTLC”. Journal of Faculty of Pharmacy of Ankara University 45/2 (Mayıs 2021), 253-263. https://doi.org/10.33483/jfpau.869819.
JAMA Bairagi S, Ghule P, Gilhotra R. IDENTIFICATION AND QUANTIFICATION OF β- SITOSTEROL IN LEAF OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA BY HPTLC. Ankara Ecz. Fak. Derg. 2021;45:253–263.
MLA Bairagi, Shripad vd. “IDENTIFICATION AND QUANTIFICATION OF β- SITOSTEROL IN LEAF OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA BY HPTLC”. Journal of Faculty of Pharmacy of Ankara University, c. 45, sy. 2, 2021, ss. 253-6, doi:10.33483/jfpau.869819.
Vancouver Bairagi S, Ghule P, Gilhotra R. IDENTIFICATION AND QUANTIFICATION OF β- SITOSTEROL IN LEAF OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA BY HPTLC. Ankara Ecz. Fak. Derg. 2021;45(2):253-6.

Cited By

Analysis of Phytochemical compounds present in some Traditional Anti-dermatophytic plants by Thin Layer, Column Chromatographic Methods
Research Journal of Pharmacy and Technology
https://doi.org/10.52711/0974-360X.2022.00305
 Kapak Resmi İndir

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.