biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 63:308-313, 2019 | DOI: 10.32615/bp.2019.035

The RNA-seq transcriptome analysis identified genes related to rice seed dormancy

K. Xie1, J. Bai1, Y.Y. Yang1, N.B. Duan1, Y.M. Ma1, T. Guo1, F.Y. Yao2, H.F. Ding1,3,*
1 Shandong Center of Crop Germplasm Resources, Jinan 250100, Shandong, P.R. China
2 Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, P.R. China
3 College of Life Science, Shandong Normal University, Jinan 250014, Shandong, P.R. China

Plant hormones play important roles in seed dormancy and dormancy breaking. We measured the hormone content in rice (Oryza sativa L. cv. Nona Bokra) seeds at different stages and with or without imbibition treatment. We identified 1 265 differentially expressed genes (DEGs) between dormant and dormancy-broken seeds using RNA-seq analysis: 1 015 genes were significantly up-regulated, while 250 genes were significantly down-regulated. Sixteen DEGs were selected as related to seed dormancy, and their expressions were validated using quantitative PCR. Three DEGs were in the same position as two reported dormancy QTLs, suggesting that they may be candidate genes that control the dormancy of rice seeds. Our study provides an important basis for cloning genes in dormant rice seeds and provides theoretical support for the study of the dormancy mechanism.

Keywords: differentially expressed genes, Oryza sativa, transcription factors

Accepted: December 8, 2018; Prepublished online: December 8, 2018; Published online: January 19, 2019  Show citation

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Xie, K., Bai, J., Yang, Y.Y., Duan, N.B., Ma, Y.M., Guo, T., Yao, F.Y., & Ding, H.F. (2019). The RNA-seq transcriptome analysis identified genes related to rice seed dormancy. Biologia plantarum, 63, Article 308-313. https://doi.org/10.32615/bp.2019.035
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