Interaction between Prenatal and Postnatal Exposure to High-Fructose Corn Syrup Increases Gene Expression of Tnfa in Hippocampus of Offspring

Mirai YAMAZAKI; Hiroya YAMADA; Eiji MUNETSUNA; Yoshitaka ANDO; Itsuki KAGEYAMA; Nao SADAMOTO; Yuki NOUCHI; Atsushi TESHIGAWARA; Genki MIZUNO; Hiroaki ISHIKAWA; Koji SUZUKI; Shuji HASHIMOTO; Koji OHASHI

doi:10.3177/jnsv.69.237

Regular Paper

Interaction between Prenatal and Postnatal Exposure to High-Fructose Corn Syrup Increases Gene Expression of Tnfa in Hippocampus of Offspring

Mirai YAMAZAKI, Hiroya YAMADA, Eiji MUNETSUNA, Yoshitaka ANDO, Itsuki KAGEYAMA, Nao SADAMOTO, Yuki NOUCHI, Atsushi TESHIGAWARA, Genki MIZUNO, Hiroaki ISHIKAWA, Koji SUZUKI, Shuji HASHIMOTO, Koji OHASHI

Author information

Mirai YAMAZAKI
Department of Medical Technology, Kagawa Prefectural University of Health Sciences
Hiroya YAMADA
Department of Hygiene, Fujita Health University School of Medicine
Eiji MUNETSUNA
Department of Biochemistry, Fujita Health University School of Medicine
Yoshitaka ANDO
Department of Clinical Biochemistry, Fujita Health University School of Medical Sciences
Itsuki KAGEYAMA
Department of Clinical Biochemistry, Fujita Health University School of Medical Sciences
Department of Preventive Medical Sciences, Fujita Health University School of Medical Sciences
Nao SADAMOTO
Department of Clinical Biochemistry, Fujita Health University School of Medical Sciences
Yuki NOUCHI
Department of Clinical Biochemistry, Fujita Health University School of Medical Sciences
Department of Preventive Medical Sciences, Fujita Health University School of Medical Sciences
Atsushi TESHIGAWARA
Department of Joint Research Laboratory of Clinical Medicine, Fujita Health University Hospital
Genki MIZUNO
Department of Preventive Medical Sciences, Fujita Health University School of Medical Sciences
Hiroaki ISHIKAWA
Department of Clinical Biochemistry, Fujita Health University School of Medical Sciences
Koji SUZUKI
Department of Preventive Medical Sciences, Fujita Health University School of Medical Sciences
Shuji HASHIMOTO
Department of Hygiene, Fujita Health University School of Medicine
Koji OHASHI
Department of Clinical Biochemistry, Fujita Health University School of Medical Sciences

Keywords: fructose, developmental origins of health and disease, fetal programming, over nutrition, inflammation

JOURNAL FREE ACCESS

2023 Volume 69 Issue 4 Pages 237-242

DOI https://doi.org/10.3177/jnsv.69.237

Details

Abstract

Concerns about the negative intergenerational effects of excessive fructose intake are being raised, with evidence suggesting that prenatal fructose intake increases susceptibility to metabolic and cognitive dysfunction later in life. In the present study, we hypothesized that prenatal and postnatal fructose intake acts synergistically to impact on hippocampus of adult offspring. Female Sprague-Dawley rats received distilled water or 20% high-fructose corn syrup (HFCS) solution in addition to standard chow throughout gestation and lactation. Male offspring were weaned at postnatal day 21 (PD21) and were randomized to receive distilled water or 20% HFCS solution until PD60. The following experimental groups were: CC: distilled water dams and post-weaning distilled water, CH: distilled water dams and post-weaning HFCS solution, HC: HFCS solution dams and post-weaning distilled water and HH: HFCS solution dams and post-weaning HFCS solution. The synergistic effect of maternal and post-weaning HFCS intake on the hippocampus was investigated by studying the expression of pro-inflammatory cytokine genes (Tnfa, Il1b, and Il6). At weaning, expression levels of pro-inflammatory cytokines between the offspring of the distilled water and HFCS solution fed dams were not significantly different. At PD60, Tnfa expression was significantly higher in the HH group than in the CC, HC and CH groups, whereas no significant differences were found between the CC, HC, and CH groups. These results suggest that postnatal fructose intake negatively impacts the hippocampus by acting synergistically with prenatal fructose intake.

Corresponding author

Register with J-STAGE for free!