Research-Article
Production of Hydrogen Peroxide by Lactobacillus acidophilus

https://doi.org/10.3168/jds.S0022-0302(80)82938-9Get rights and content
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Abstract

Hydrogen peroxide production in reconstituted skim milk (10%) and low-fat milk by four strains of Lactobacillus acidophilus was studied at 37 and 4 C. Strains A and B produced little, but strains C and D produced larger amounts, especially if agitated continuously during growth at 37 C or storage at 4 C. Continuous shaking was required at 4 C for C or D (4.0 × 108/ml) to produce sufficient hydrogen peroxide to retard growth of Pseudomonas fragi. Flavin adenine dinucleotide stimulated the oxidation of reduced nicotinamide adenine dinucleotide by dialyzed cell-free extracts of C and D, which indicated that the reduced nicotinamide adenine dinucleotide oxidases of these strains produce hydrogen peroxide as an end product.

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