The primary objective of this randomized controlled challenge study was to investigate the effect of ampicillin on ultrasonographic (US) lung consolidation after experimental challenge with Pasteurella multocida in preweaned dairy calves. The secondary objectives were to determine whether ampicillin affected respiratory score, gross consolidation, or the detection of P. multocida in lung tissue at postmortem exam (PME). Holstein bull calves (n = 39) were transported to the University of Wisconsin-Madison School of Veterinary Medicine isolation facility at the mean (±SD) age of 52 ± 6 d. After a 7-d acclimation period, 30 calves were inoculated intratracheally with 1010 cfu of ampicillin-sensitive P. multocida. Lung US and respiratory scoring were performed 2, 6, 12, and 24 h post-challenge, then US once daily and respiratory scoring twice daily until d 14. Calves were randomized to receive ampicillin [n = 17, treatment (TX), 6.6 mg/kg i.m. once daily for 3 d] or placebo [n = 11, control (CON), saline, equal volume, i.m. once daily for 3 d] when ≥1 cm2 of lung consolidation was observed and ≥6 h had elapsed since challenge. Lung lesions ≥1 cm2 were considered positive for consolidation. Calves were respiratory score positive if ≥2 in 2 or more categories based on the Wisconsin respiratory health score chart. Area under the curve (AUC) was calculated for US score and respiratory score as a proxy for time with consolidation and clinical respiratory disease, respectively. Gross lung lesions and pathogens were quantified following PME. At the time of first treatment, consolidation had developed in 28/30 calves (TX, n = 17; CON, n = 11) and 6% (1 out of 17) of TX and 9% (1 out of 11) of CON calves had a positive respiratory score. The TX calves had a significantly lower median (interquartile range given in parentheses) AUC for US score [TX: 23 (20, 29), CON: 47 (33, 53)], whereas mean AUC for respiratory score was not different between groups (TX: 93 ± 28, CON: 96 ± 17). On d 14, 70% (12 out of 17) of TX and 100% (11 out of 11) of CON calves had lung consolidation, and 24% (4 out of 17) of TX and 27% (3 out of 11) of CON calves had clinical respiratory disease. On PME, median consolidation was 10% (6, 15) for TX and 10% (2, 28) for CON calves. Lung cultures were positive for P. multocida in 77% (13 out of 17) of TX and 91% (10 out of 11) of CON calves. Lung health benefited from a 3-d ampicillin therapy, but benefits were short-lived. Treatment failures might be due to incomplete resolution of the initial lung infection. Future studies are needed to optimize TX strategies to improve long-term lung health.
