Pain-reducing anesthesia prevents oxidative stress in human term placenta

Yoko Tsuzuki; Yoriko Yamashita; Yuka Hattori; Guang Hua Li; Shinya Akatsuka; Tomomi Kotani; Fumitaka Kikkawa; Aya Naiki-Ito; Satoru Takahashi; Kimitoshi Nishiwaki; Shinya Toyokuni

doi:10.3164/jcbn.15-138

Original Articles

Pain-reducing anesthesia prevents oxidative stress in human term placenta

Yoko Tsuzuki, Yoriko Yamashita, Yuka Hattori, Guang Hua Li, Shinya Akatsuka, Tomomi Kotani, Fumitaka Kikkawa, Aya Naiki-Ito, Satoru Takahashi, Kimitoshi Nishiwaki, Shinya Toyokuni

Author information

Yoko Tsuzuki
Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine
Department of Anesthesiology and Resuscitation, Nagoya University Graduate School of Medicine
Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences
Yoriko Yamashita
Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine
Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences
Yuka Hattori
Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine
Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine
Guang Hua Li
Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine
Shinya Akatsuka
Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine
Tomomi Kotani
Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine
Fumitaka Kikkawa
Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine
Aya Naiki-Ito
Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences
Satoru Takahashi
Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences
Kimitoshi Nishiwaki
Department of Anesthesiology and Resuscitation, Nagoya University Graduate School of Medicine
Shinya Toyokuni
Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine

Keywords: oxidative stress, placenta, anesthesia, 8-hydroxy-2'-deoxyguanosine, 4-hydroxy-2-nonenal-modified proteins

JOURNAL FREE ACCESS

2016 Volume 58 Issue 2 Pages 156-160

DOI https://doi.org/10.3164/jcbn.15-138

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Abstract

Anesthesia is sometimes used for the reduction of maternal pain in normal human term labor, but whether the drugs affect oxidative stress remains unclear. The placenta serves as an interface between the maternal and fetal vasculature. In this study, we immunohistochemically analyzed two markers for oxidative stress, namely 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 4-hydroxy-2-nonenal–modified proteins (HNE), using placentas from 21 cases of normal tansvaginal delivery (V group), 20 Caesarean sections (C group), and 17 normal transvaginal deliveries with epidural anesthesia (E group). 8-OHdG staining in the nuclei of trophoblasts lining the chorionic villi was significantly stronger in the V group either compared with the C or E group (p<0.001), without significant differences in the C and E groups (p = 0.792). Moderate to intense staining by HNE of the intravascular serum of chorionic villi vasculature was frequently observed in the placentas from the V group, but less frequently of those in either C or E groups (p<0.001), nor the p value comparing the C and E groups was significant (p = 0.128) for HNE staining. Our results suggest that although the role of oxidative stress and its influences on fetal state in the placenta in labor remains unclear, it seems to be lessened by epidural anesthesia.

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