Characterization of Commercial Polysorbates Using Different Chromatographic Techniques

S. Abrar; B. Trathnigg

doi:10.3139/113.110032

Published by De Gruyter April 2, 2013

Characterization of Commercial Polysorbates Using Different Chromatographic Techniques

Charakterisierung handelsüblicher Polysorbate mittels verschiedener Chromatographietechniken

S. Abrar and B. Trathnigg

From the journal Tenside Surfactants Detergents

https://doi.org/10.3139/113.110032

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Abstract

Polysorbates – which are commercially available under the name Tween – are complex mixtures of different functionalities. Molar mass distribution and chemical composition of various Tween samples (20, 40, 60 and 65) were determined using size exclusion chromatography (SEC) with dual detection. A separation of different functionalities was achieved using liquid chromatography under critical conditions (LCCC) on typical reversed phase columns with methanol – water and acetone – water as mobile phase. The size of the hydrophilic part was determined after microwave-assisted methanolysis, which allowed also the identification of the fatty acids bound to the hydrophilic core.

Kurzfassung

Polysorbate (im Handel unter dem Namen Tween) sind komplexe Mischungen von Polymeren unterschiedlicher Funktionalität. Molmassenverteilung und chemische Zusammensetzung verschiedener Proben (Tween 20, 40, 60 und 65) wurden mittels Größenausschlusschromatographie (SEC) mit Doppeldetektion bestimmt. Eine Trennung nach Funktionalität erfolgte durch Flüssigkeitschromatographie unter kritischen Bedingungen (LCCC) an typischen Umkehrphasen in Methanol – Wasser und Aceton – Wasser als mobile Phase. Die Größe des hydrophilen Teils wurde nach Mikrowellen-unterstützter Methanolyse bestimmt. Auf diese Weise konnten auch die an den hydrophilen Kern gebundenen Fettsäuren identifiziert werden.

Key words:: Polysorbate; Tween; size exclusion chromatography; dual detection; liquid chromatography under critical conditions; microwave

Stichwörter:: Polysorbat; Tween; Größenausschlusschromatographie; Doppeldetektion; Flüssigkeitschromatographie unter kritischen Bedingungen; Mikrowelle

^∗ Bernd Trathnigg, Institute of Chemistry, Karl-Franzens-University, Heinrichstrasse 28, Central Polymer Laboratory/Molecular Characteristics (CePol/MC), A-8010 Graz, Austria, Fax: +433163809840, E-Mail: bernd.trathnigg@uni-graz.at

Shazia Abrar born in April 24, 1984. She completed her M.Sc. Chemistry in 2003 and M.Phill. Chemistry in 2005 from University of Agriculture Faisalabad, Pakistan. Currently, she is working as PhD student with Prof. Dr. Bernd Trathnigg in University of Graz Austria.

Prof. Dr. phil. Bernd Trathnigg studied chemistry at Karl-Franzens-University in Graz from 1965 to 1973, specializing in polymer chemistry. After receiving his PhD “sub auspiciis praesidentis rei publicae” he spent one year at the Austrian Research Center for Atomic Energy in Seibersdorf working as coordinator of a project on electron beam curing of paints. 1974 he went back to the Institute of Chemistry, Karl-Franzens University Graz, where he received the title of an associate professor in 1990. He has developed of a density detector for liquid chromatography. Now he works mainly on characterization of polymers by liquid chromatography with special emphasis on quantitation, hyphenated techniques in analysis of polymers and surfactants, including 2D-HPLC.

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Received: 2009-03-05

Revised: 2009-06-10

Published Online: 2013-04-02

Published in Print: 2009-09-01

Characterization of Commercial Polysorbates Using Different Chromatographic Techniques

Abstract

Kurzfassung

References

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