Abstract
Fluorescent nanoparticles are currently considered as promising enhancers of photodynamic activity of traditional photosensitizers in procedures for photodynamic therapy of cancer and inactivation of pathogens. On the one hand, such nanoparticles act as a nanoplatform for target delivery of dye molecules to cells; on the other hand, they act as light-harvesting antennas that increase the effective absorption cross-section of the dye. This paper investigates the effect of semiconductor CdSe/ZnS quantum dots coated with a polymer shell on the photostability of zinc or aluminum polycationic phthalocyanines. It was shown that the rate of photobleaching of phthalocyanines increases significantly in the presence of quantum dots both under direct illumination of phthalocyanine by red light and under the quantum dot-mediated illumination by blue light. This effect can be explained by the fact that phthalocyanine inside the polymer shell of the quantum dot is the primary target for the attack by reactive oxygen species. The rate of bleaching of the chemical trap of reactive oxygen species, 4-nitroso-N,N-dimethylaniline, in a phthalocyanine solution increases in the presence of the quantum dot under red light illumination. We believe that the chemical trap is concentrated in the polymer shell of the quantum dot, which increases the probability of its damage by active oxygen species generated by phthalocyanine. Since the diffusion of reactive oxygen species from the polymer shell of the nanoparticle into the surrounding solution is slow, the use of quantum dots as an enhancer of the photodynamic action of phthalocyanines can be effective only in the absence of significant steric obstacles to the diffusion of reactive oxygen species to the target molecules of photodynamic inactivation.
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REFERENCES
De Annunzio, S.R., Costa, N.C.S., Mezzina, R.D., Graminha, M.A.S., and Fontana, C.R., Chlorin, phthalocyanine, and porphyrin types derivatives in phototreatment of cutaneous manifestations: A review, Int. J. Mol. Sci., 2019, vol. 20, no. 16, p. 3861.
Ghorbani, J., Rahban, D., Aghamiri, S., Teymouri, A., and Bahador, A., Photosensitizers in antibacterial photodynamic therapy: An overview, Laser Ther., 2018, vol. 27, no. 4, pp. 293–302.
Martinez De Pinillos Bayona, A., Mroz, P., Thunshelle, C., and Hamblin, M.R., Design features for optimization of tetrapyrrole macrocycles as antimicrobial and anticancer photosensitizers, Chem. Biol. Drug Des., 2017, vol. 89, no. 2, pp. 192–206.
Bechet, D., Couleaud, P., Frochot, C., Viriot, M.L., Guillemin, F., and Barberi-Heyob, M., Nanoparticles as vehicles for delivery of photodynamic therapy agents, Trends Biotechnol., 2008, vol. 26, no. 11, pp. 612–621.
Samia, A.C.S., Dayal, S., and Burda, C., Quantum dot-based energy transfer: Perspectives and potential for applications in photodynamic therapy, Photochem. Photobiol., 2006, vol. 82, no. 3, pp. 617–625.
Kraljic, I. and Moshni, S.E., A new method for the detection of singlet oxygen in aqueous solutions, Photochem. Photobiol., 1978, vol. 28, pp. 577–581.
Gvozdev, D.A., Maksimov, E.G., Strakhovskaya, M.G., Ivanov, M.V., Paschenko, V.Z., and Rubin, A.B., The effect of ionic strength on spectral properties of quantum dots and aluminum phthalocyanine complexes, Nanotechnol. Russ., 2017, vol. 12, nos. 1–2, pp. 73–85.
Kuznetsova, N.A., Makarov, D.A., Yuzhakova, O.A., Solovieva, L.I., and Kaliya, O.L., Study on the photostability of water-soluble Zn (II) and Al (III) phthalocyanines in aqueous solution, J. Porphyr. Phthalocyanines, 2010, vol. 14, no. 11, pp. 968–974.
Krasnovsky, A.A., Jr., Singlet molecular oxygen in photobiochemical systems: IR phosphorescnence studies, Membr. Cell Biol., 1998, vol. 12, no. 5, pp. 665–690.
Zaitseva, S.V., Zdanovich, S.A., and Koifman, O.I., Coordination properties of (chloro)aluminum-5,15-diphenyloctaalkylporphyrin in the reactions with small organic molecules, Russ. J. Coord. Chem., 2010, vol. 36, no. 5, pp. 323–329.
Gvozdev, D.A., Maksimov, E.G., Strakhovskaya, M.G., Moysenovich, A.M., Ramonova, A.A., Moisenovich, M.M., Goryachev, S.N., Paschenko, V.Z., and Rubin, A.B., A CdSe/ZnS quantum dot-based platform for the delivery of aluminum phthalocyanines to bacterial cells, J. Photochem. Photobiol. B, Biol., 2018, vol. 187, pp. 170–179.
Martynenko, I.V., Orlova, A.O., Maslov, V.G., Fedorov, A.V., Berwick, K., and Baranov, A.V., The influence of phthalocyanine aggregation in complexes with CdSe/ZnS quantum dots on the photophysical properties of the complexes, Beilstein J. Nanotechnol., 2016, vol. 7, no. 1, pp. 1018–1027.
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Translated by K. Lazarev
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Gvozdev, D.A., Maksimov, E.G. & Paschenko, V.Z. Photobleaching of Phthalocyanine Molecules within a Complex with Colloidal Quantum Dots. Moscow Univ. Biol.Sci. Bull. 75, 7–12 (2020). https://doi.org/10.3103/S0096392520010022
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DOI: https://doi.org/10.3103/S0096392520010022