Abstract
Analysis of the expression of genes encoding myelin basic protein (MBP) and glial fibrillary acidic protein (GFAP) in human glial tumors was carried out for determination of the expression specificity of these genes according to tumor types and their malignancy. Low levels of MBP mRNA in astrocytoma specimens of malignancy grades II-IV and significantly higher levels in perifocal zones adjacent to them have been determined by Northern hybridization. Diffuse astrocytomas and anaplastic astrocytomas are characterized mostly by a low level of MBP gene expression and high level of GFAP gene expression, but distinct subtypes of diffuse and anaplastic astrocytomas with a high level of GFAP gene expression can also be detected that may be the reflection of different oncogenic pathways. Very low levels or even absence of MBP mRNA were revealed in oligodendroglioma and all oligoastrocytomas. Thus, Northern hybridization data are correlated with serial analysis of gene expression (SAGE). Obtained results show that MBP is a nonspecific marker for tumors of oligodendroglial origin, but determination of relative levels of MBP and GFAP mRNAs may be useful for glial tumor recognition. In such a way, these two genes together with YKL-40 and TSC-22, which we found previously, can be included into the gene panel for determination of so-called “gene signatures” of brain tumors. However, strict requirements in relation to a clinical value of these “gene signatures” cannot be formulated without verifying them on a large number of clinical samples of tumors and valid control.
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Louis, D.N., Ohgaki, H., Wiestler, O.D., Cavenee, W.K., Burger, P.C., Jouvet, A., Scheithauer, B.V., and Kleihues, P., The 2007 WHO Classification of Tumours of the Central Nervous System, Acta Neuropathol., 2007, vol. 114, no. 2, pp. 97–109.
McDonald, J.M., See, S.J., Tremont, I.W., Colman, H., Gilbert, M.R., Groves, M., Burger, P.C., Louis, D.N., Giannini, C., Fuller, G., Passe, S., Blair, H., Jenkins, R.B., Yang, H., Ledoux, A., Aaron, J., Tipnis, U., Zhang, W., Hess, K., and Aldape, K., The Prognostic Impact of Histology and 1p/19q Status in Anaplastic Oligodendroglial Tumors, Cancer, 2005, vol. 104, no. 7, pp. 1468–1477.
Schmidt, M.C., Antweiler, S., Urban, N., Mueller, W., Kuklik, A., Meyer-Puttlitz, B., Weistler, O.D., Louis, D.N., Fimmers, R., and von Deimling, A., Impact of Genotype and Morphology on the Prognosis of Glioblastoma, J. Neuropathol. Exp. Neurol., 2002, vol. 61, no. 4, pp. 321–328.
Baumann, N., and Pham-Dinh, D., Biology of Oligodendrocyte and Myelin in the Mammalian Central Nervous System, Physiol. Rev., 2001, vol. 81, no. 2, pp. 871–927.
Dmitrenko, V.V., Boyko, O.I., Shostak, K.O., Vitak, N.Ya., Bukreyeva, T.V., Rozumenko, V.D., Malysheva, T.A., Shamayev, M.I., Zozulya, Y.P., and Kavsan, V.M., Characterization of Genes with Decreased Expression in Human Gliomas-Potential Tumor Suppressor Genes, Biopolymers and Genes, 2007, vol. 23, no. 4, pp. 347–362.
Landry, C.F., Verity, M.A., Cherman, L., Kashima, T., Black, K., Yates, A., and Campagnoni, A.T., Expression of Oligodendrocytic mRNAs in Glial Tumors: Changes Associated with Tumor Grade and Extent of Neoplastic Infiltration, Cancer Res., 1997, vol. 57, pp. 4098–4104.
Golfinos, J.G., Norman, S.A., Coons, S.W., Norman, R.A., Ballecer, C., and Scheck, A.C., Expression of the Genes Encoding Myelin Basic Protein and Proteolipid Protein in Human Malignant Gliomas, Clin. Cancer Res., 1997, vol. 3, no. 5, pp. 799–804.
Popko, B., Pearl, D.K., Walker, D.M., Comas, T.C., Baerwald, K.D., Burger, P.C., Scheithauer, B.W., and Yates, A.J., Molecular Markers that Identify Human Astrocytomas and Oligodendrogliomas, J. Neuropathol. Exp. Neurol., 2002, vol. 61, no. 4, pp. 329–338.
Figols, J., Iglesias-Rozas, J.R., and Kazner, E., Myelin Basic Protein (MBP) in Human Gliomas: a Study of Twenty-Five Cases, Clin. Neuropathol., 1985, vol. 4, no. 3, pp. 116–120.
Nakagawa, Y., Perentes, E., and Rubinstein, L.J., Immunohistochemical Characterization of Oligodendrogliomas: an Analysis of Multiple Markers, Acta Neuropathol., 1986, vol. 72, pp. 15–20.
Tanaka, J., Hokama, Y., and Nakamura, H., Myelin Basic Protein as a Possible Marker for Oligodendroglioma, Acta Pathol. Japan., 1988, vol. 38, no. 10, pp. 1297–1303.
Eng, L.F., Vanderhaeghen, J.J., Bignami, A., and Gerstl, B., An Acidic Protein Isolated from Fibrous Astrocytes, Brain Res., 1971, vol. 28, pp. 351–354.
Eng, L.F. and Lee, Y.L., Intermediate Filaments in Astrocytes Neuroglia, New York: Oxford Univ. Press, 1995, pp. 650–667.
Mapstone, T.B. and Galloway, P.G., Expression of Glial Fibrillary Acidic Protein, Vimentin, Fibronectin, and Nmyc Oncoprotein in Primary Human Brain Tumor Cell Explants, Pediatr. Neurosurg., 1991/1992, vol. 17, no. 4, pp. 169–174.
Studer, A., de Tribolet, N., Diserens, A.C., Gaide, A.C., Matthieu, J.M., Carrel, S., and Stavrou, D., Characterization of Four Human Glioma Cell Lines, Acta Neuropathol., 1985, vol. 66, no. 3, pp. 208–217.
Velasko, M.E., Dahl, D., Roessmann, U., and Gambetti, P., Immunohistochemical localization of Glial Fibrillary Acidic Protein in Human Glial Neoplasms, Cancer, 1980, vol. 45, pp. 484–494.
Herpers, M.J. and Budka, H., Glial Fibrillary Acidic Protein (GFAP) in Oligodendroglial Tumors: Gliofibrillary Oligodendroglioma and Transitional Oligoastrocytoma as Subtypes of Oligodendroglioma, Acta Neuropathol. (Berlin), 1984, vol. 64, pp. 265–272.
Taskos, N.A., Parr, J., and Gonatas, N.K., Immunocytochemical Study of the Glial Fibrillary Acidic Protein in Human Neoplasms of the Central Nervous System, Hum. Pathol., 1982, vol. 13, pp. 454–458.
Van der Meulen, J.D.M., Houthoff, H.J., and Ebels, E.J., Glial Fibrillary Acidic Protein in Human Gliomas, Neuropathol. Appl. Neurobiol., 1978, vol. 4, pp. 177–190.
Chomchinsky, P. and Sacchi, N., Single Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol Chlorophorm Extraction, Anal. Biochem., 1987, vol. 162, no. 1, pp. 156–159.
Sambrook, J., Fritch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 1989.
Kavsan, V.M., Shostak, K.O., Dmitrenko, V.V., Zozulya, Y., Rozumenko, V., and Demotes-Mainard, J., Characterization of Genes with Increased Expression in Human Glioblastomas, Tsitol. Genet., 2005, vol. 39, no. 6, pp. 37–49.
Kavsan, V.M., Dmitrenko, V.V., Shostak, K.O., Bukreyeva, T.V., Vitak, N.Y., Simirenko, O.E., Malisheva, T.A., Shamayev, M.I., Rozumenko, V.D., and Zozulya, Y.A., Comparison of Microarray and SAGE Techniques in Gene Expression Analysis of Human Glioblastoma, Tsitol. Genet., 2007, vol. 41, no. 1, pp. 36–55.
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Original Russian Text © V.V. Dmitrenko, O.I. Boyko, K.O. Shostak, A.V. Beletskii, T.A. Malisheva, M.I. Shamayev, V.M. Klyuchka, V.D. Rozumenko, Y.P. Zozulya, V.M. Kavsan, 2009, published in Tsitologiya i Genetika, 2009, Vol. 43, No. 1, pp. 30–37.
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Dmitrenko, V.V., Boyko, O.I., Shostak, K.O. et al. Expression of myelin basic protein and glial fibrillary acidic protein genes in human glial tumors. Cytol. Genet. 43, 22–27 (2009). https://doi.org/10.3103/S0095452709010046
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DOI: https://doi.org/10.3103/S0095452709010046