Abstract
Intracellular distribution of assimilated 2,4,6-trinitrotoluene (TNT) in callus cells, flower buds, and leaves of intact Yucca gloriosa L. plants with the use of electron microscopy radioautography was carried out. The radiotracer was detected in vacuoles, plastids, mitochondria, endoplasmic reticulum, and cytoplasm. It was found that TNT incorporation in the vacuoles of dedifferentiated callus cells was higher compared to cells from intact plant. Therefore, the ultrastructural continuity of differentiated cells is less damaged.
Similar content being viewed by others
References
Mueller, W.F., Bedell, G.W., Shojaee, S., and Jackson, P.J., Bioremediation of TNT Wastes by Higher Plants, Proc. 10th Ann. Conf. on Hazardous Waste Res., 1999, pp. 222–230.
Khatisashvili, G., Kvesitadze, G., Adamia, G., Gagelidze, N., Sulamanidze, L., Ugrekhelidze, D., Zaalishvily, G., Ghoghoberidze, M., and Ramishvili, M., Bioremediation of Contaminated Soil on the Former Military Locations and Proving Grounds in Georgia, J. Biol. Physics and Chem., 2004, vol. 4, pp. 162–168.
Gong, P., Wilke, B.M., and Fleischmann, S., Soil-Based Phytotoxicity of 2,4,6-Trinitrotoluene (TNT) to Terrestrial Higher Plant, Arch. Environ. Cont. Toxicol., 1999, vol. 36, pp. 152–157.
Adamia, G., Ghoghoberidze, M., Graves, D., Khatisashvili, G., Kvesitadze, G., and Ugrechelidze, D., Absorption, Distribution and Transformation of TNT in Higher Plants, Ecotoxicol. Environm. Safety, 2006, vol. 64, pp. 136–145.
Ramishvili, M., Gogava, M., Chelidze, N., and Ghoghoberidze, M., Study of 2,4,6-Trinitroluene (TNT) Assimilation Ability of Cells of Yucca Gloriosa L. In vitro Culture, Proc. Georgian Acad. Sci. B., 2005, vol. 3, no. 2, pp. 21–25.
Gogava, M., Ramishvili, M., Ghoghoberidze, M., Kurashvili, M., Pruidze, M., and Chelidze, N., TNT Effect on the Nitroreduktase, Peroksidase and Phenoloxidase Activities In Vitro Cultures of Yucca Gloriosa and Glycine Max, Proc. Georgian Acad. Sci. B, 2006, vol. 4, no. 4, pp. 1–5.
Meskhi, A.B., Ghoghoberidze, M., and Katsidadze, K.P., Cell Culture of Yucca Gloriosa L. Izvestiya AN GSSR. Ser. Biol. (Bull. Acad. Sci. of Georgian SSR. Ser. Biol.), 1978, vol. 4, no. 1, pp. 182–186.
Gayer, G., Electronnaya gistokhimiya (Electron Histochemistry), Moscow: Mir, 1974, p. 488.
Murashidze, T. and Skoog, F.A., Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures, Physiol. Plant, 1962, vol. 15, pp. 473–497.
Buadze, O.A., Durmishidze, S.V., and Apakidze, A.V., Ultrastructural Aspects of Herbicide 2,4-D Movement and Localization in Plant Cells, Bull. Georg. Acad. Sci., 1985, vol. 119, pp. 4109–4411.
Kvesitadze, G., Khatisashvili, G., Sadunishvili, T., and Ramsden, J.J., Biochemicals Mechanisms of Detoxification in Higher Plants: Basics of Phytoremediation, Berlin: Springer, 2006, pp. 262–267.
Zaalishvili, G.V., Varasashvili, T.G., Ananiashvili, T.I., Khatisashvili, G.A., and Kvesitadze, E.G., Ultrastructure Reorganization of Plant Cell in the Process of Metabolism of Xenobiotics, Izv. Agrar. Nauki, 2005, vol. 3, no. 3, pp.117–122.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © M. Ghoghoberidze, G. Zaalishvili, M. Ramishvili, M. Gogava, N. Chelidze, 2009, published in Tsitologiya i Genetika, 2009, Vol. 43, No. 1, pp. 25–29.
About this article
Cite this article
Ghoghoberidze, M., Zaalishvili, G., Ramishvili, M. et al. Ultrastructural study of the effect of TNT on callus cells and cells of intact plants of Yucca gloriosa L.. Cytol. Genet. 43, 18–21 (2009). https://doi.org/10.3103/S0095452709010034
Received:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S0095452709010034