Abstract
The presence of viable hydrocarbon-oxidizing microorganisms has been shown in the under-ground waters exposed by the Vorotilovskaya deep well (the Puchezh-Katunki astrobleme, 75 km north of Nizhny Novgorod, 1900- and 3200-m deep) using the method of chromatography-mass spectrometry of specific biomarkers of the microbial cell wall and the classical methods of bacteriology. Several microbial species have been isolated in pure culture and identified. Two bacillary species, Bacillus pumilus KTB-2 and Bacillus subtilis KTB-4, were maintained in pure cultures at reinoculations. The effects of mineralization and aeration of the medium on the growth characteristics of Bacillus pumilus KTB-2 in batch culture have been studied.
References
Nazina, T.N., Sokolova, D.Sh., Grigor’yan, A.A., Syue, Ya.F., Belyaev, S.S., and Ivanov, M.V., Production of oil-releasing compounds by microorganisms from the Daqing oil field, China, Microbiology, 2003, vol. 72, no. 2, pp. 173–178.
Nazina, T.N., Ivanova, A.E., Ivoilov, V.S., Kandaurova, G.F., Ibatullin, R.R., Belyaev, S.S., and Ivanov, M.V., Microbiological investigation of the stratal waters of the Romashkinskoe oil field in the course of a trial of a biotechnology for the enhancement of oil recovery, Microbiology, 1999, vol. 68, no. 2, pp. 214–221.
Vorontsov, A.K. and Rabinovich, Yu.I., Chemical composition and gases of underground waters of the central uplift of the Puchezh-Katun astrobleme according to the results of drilling the Vorotilovskaya well, Razved. Okhr. Nedr, 1996, no. 7, pp. 19–23.
Kondakova, G.V., Verkhovtseva, N.V., and Osipov, G.A., Bacterial processes of carbon and nitrogen cycles in deep waters of the lithosphere, Vestn. Mosk. Univ., Ser. 16: Biol., 2002, no. 4, pp. 26–33.
Kondakova, G.V., Verkhovtseva, N.V., and Osipov, G.A., Study of microbial diversity of underground waters during monitoring of deep horizons of Earth’s crust, Vestnik Mosk. Univ., Ser. 16: Biol., 2007, no. 2, pp. 23–29.
Osipov, G.A., RF Patent No. 2086642, 1993.
Verkhovtseva, N.V. and Osipov, G.A., Method of gas chromatography-mass spectrometry in investigation of soil microbial communities of agrocenoses, Probl. Agrokhim. Ekol., 2008, no. 1, pp. 51–54.
Verkhovtseva, N.V. and Kondakova, G.V., Microbiolkogical sstudies of rocks and underground waters, in Sverkhglubokoe burenie Puchezh-Katunkskoi impaktnoi struktury (Ultradeep Drilling of the Puchezh-Katun Impact Structure), St. Petersburg: VSEGEI, 1999, pp. 176–186.
Kuznetsov, S.I. and Dubinina, G.A., Metody izucheniya vodnykh mikroorganizmov (Methods of Investigation of Aquatic Microorganisms), Moscow: Nauka, 1989.
Metody pochvennoi mikrobiologii i biokhimii (Methods of Soil Microbiology and Biochemistry), Zvyagintsev, D.G., Ed., Moscow: Izd. Mosk. Gos. Univ., 1991.
Panikov, N.S., Kinetika rosta mikroorganizmov (Micro-organism Growth Kinetics), Moscow: Nauka, 1991.
Ivshina, I.B., Alkanotrophic rhodococci: biodiversity, detection, and species differentiation, in Mikrobnoe raznoobrazie: sostoyanie, strategiya sokhraneniya, ekologicheskie problemy: Mater. Mezhdunar. Konf., Perm, 8–11 okt. 1996 (Microbial Diversity: State, Strategy, Conservation Strategy, and Ecological Problems: Proc. Int. Conf., Perm, October 8–11, 1996), Perm: Inst. Ekol. Genet. Mikroorgan. Ural. Otd. Ros. Acad. Nauk, 1996, p. 37.
Balkwill, D.L. and Boone, D.R., Identify and diversity of microorganisms cultured from subsurface environments, in The Microbiology of the Terrestrial Deep Subsurface, New York: Lewis Publishers, 1997, pp. 105–117.
Oborin, A.A., Rubinshtein, L.M., Khmurchik, V.T., and Churilova, N.S., Kontseptsiya organizovannosti podzemnoi biosfery (Concept of Subsurface Biosphere Organization), Yekaterinburg: Ural. Otd. Ross. Acad. Nauk, 2004.
Phels, T.J., Murphy, E.M., Pfiffner, S.M., and White, D.C., Comparison between geochemical and biological estimates of subsurface microbial activities, Microb. Ecol., 1994, vol. 28, pp. 335–349.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © G.V. Kondakova, N.V. Verkhovtseva, S.A. Ostroumov, 2013, published in Vestnik Moskovskogo Universiteta. Biologiya, 2013, No. 3, pp. 31–35.
About this article
Cite this article
Kondakova, G.V., Verkhovtseva, N.V. & Ostroumov, S.A. Study of hydrocarbon oxidizing microorganisms from deep groundwater of the Puchezh-Katunki impact structure. Moscow Univ. Biol.Sci. Bull. 68, 119–123 (2013). https://doi.org/10.3103/S0096392513030061
Received:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S0096392513030061