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Study of hydrocarbon oxidizing microorganisms from deep groundwater of the Puchezh-Katunki impact structure

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Abstract

The presence of viable hydrocarbon-oxidizing microorganisms has been shown in the under-ground waters exposed by the Vorotilovskaya deep well (the Puchezh-Katunki astrobleme, 75 km north of Nizhny Novgorod, 1900- and 3200-m deep) using the method of chromatography-mass spectrometry of specific biomarkers of the microbial cell wall and the classical methods of bacteriology. Several microbial species have been isolated in pure culture and identified. Two bacillary species, Bacillus pumilus KTB-2 and Bacillus subtilis KTB-4, were maintained in pure cultures at reinoculations. The effects of mineralization and aeration of the medium on the growth characteristics of Bacillus pumilus KTB-2 in batch culture have been studied.

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Correspondence to G. V. Kondakova.

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Original Russian Text © G.V. Kondakova, N.V. Verkhovtseva, S.A. Ostroumov, 2013, published in Vestnik Moskovskogo Universiteta. Biologiya, 2013, No. 3, pp. 31–35.

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Kondakova, G.V., Verkhovtseva, N.V. & Ostroumov, S.A. Study of hydrocarbon oxidizing microorganisms from deep groundwater of the Puchezh-Katunki impact structure. Moscow Univ. Biol.Sci. Bull. 68, 119–123 (2013). https://doi.org/10.3103/S0096392513030061

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  • DOI: https://doi.org/10.3103/S0096392513030061

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