نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد، دانشکده علوم و فنون نوین، دانشگاه تهران، تهران، تهران، ایران
2 دانشیار، دانشکده علوم و فنون نوین، دانشگاه تهران، تهران، تهران، ایران
3 دانشجوی کارشناسی ارشد، دانشکده فنی و مهندسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، تهران، ایران
چکیده
هدف از این پژوهش، ساخت و بررسی خواص داربست نانولیفی از پلیکاپرولاکتون- فیبروئین ابریشم سولفونه برای کاربردهای مهندسی بافت استخوان است. بدینمنظور، فیبروئین ابریشم، بعد از تخلیص از پیله ابریشم، با استفاده از پیریدین و کلروسولفونیک اسید، سولفونه شد. سپس، مقدار مشخصی از فیبروئین ابریشم سولفونه، با پلیکاپرولاکتون مخلوط و با ولتاژ ۱۱ کیلوولت و سرعت ۴/۰ میلیلیتر بر ساعت الکتروریسی شد. آزمون های مختلفی مانند بررسی ترکیب شیمیایی، ساختار، زیستفعالی، سمّیت و چسبندگی سلولی انجام شد. با بررسی طیفسنجی تبدیل فوریه فروسرخ، مشخص شد که گروههای سولفاتی و سولفوناتی، با موفقیت، در ساختار فیبروئین ابریشم ایجاد شدهاند. تصاویر میکروسکوپ الکترونی روبشی، حاکی از تشکیل ساختاری لیفیشکل با الیاف پیوسته و بدون گره در ترکیبات الکتروریسی شده بود. افزودن فیبروئین ابریشم سولفونهشده به پلیکاپرولاکتون، سبب کاهش قطر الیاف از ۲۴۴ نانومتر به ۱۳۸ نانومتر شد. همچنین، نتایج نشان داد که میزان جذب آب در داربست پلیکاپرولاکتون، براثر افزودن فیبروئین ابریشم سولفونه، به ترتیب از ۴/۱۲ درصد به ۱۶۷ درصد افزایش یافته است. نتایج بهدستآمده از آزمونهای زیستفعالی و کشت سلولی حاکی از آن بود که فیبروئین ابریشم سولفونه، سبب رسوب لایه کلسیم فسفات آپاتیتی و همچنین، بهبود تکثیر و چسبندگی سلولی در الیاف پلیکاپرولاکتون شده است.
کلیدواژهها
موضوعات
عنوان مقاله [English]
Preparation and Characterization of Composite Bone Scaffold Based on Polycaprolactone and Sulfonated Silk Fibroin Nanofibers
نویسندگان [English]
- Negin Vahedi 1
- Jhamak Nourmohammadi 2
- Niloofar Pahlevani 3
- Sahar Nakhostin Hosseini 1
1 M. Sc. Student, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Tehran, Iran
2 Associate Professor, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Tehran, Iran
3 M. Sc. Student, Faculty of Technology and Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Tehran, Iran
چکیده [English]
The objective of this study was to fabricate and characterize the characteristics of polycaprolactone-sulfonated silk fibroin nanofibrous scaffold for bone tissue engineering applications. Thus, after extraction of silk fibroin (SF) from from Bombyx mori cocoons, silk fibroin (SF) was sulfonated using chlorosulfonic acid and pyridine. Afterwards, a certain amount of sulfonated SF was mixed with polycaprolactone (PCL) solution, and then electrospinning was done using 11 kV high voltage and feeding rate 0.4 mL/h. Various characterization tests were applied to analyze such items such as the structure, chemical composition, bioactivity, cellular attachment, and viability. Fourier transform infrared spectroscopy analyses proved the successful incorporation of sulfate and sulfonate groups in SF structure. The scanning electron microscope shows the formation of continuous and beadless fibers. The average fiber diameter in polycaprolactone nanofibers reduced from 244 nm to 138 nm with the addition of sulfonated SF. Moreover, the water uptake of PCL nanofibers improved from 12.4 % to 167 % after the addition of sulfonated silk fibroin to polycaprolactone. The results of bioactivity and cell culture experiments indicated that sulfonated SF promotes apatitic calcium phosphate deposition and also enhances cellular attachment and viability of PCL nanofibers.
کلیدواژهها [English]
- Polycaprolactone
- Sulfonated Silk Fibroin
- Calcium Phosphate Deposition
- Bone Scaffold
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