ارزیابی نوع ریزنمونه و فصل نمونه‌گیری تحت تأثیر تیمارهای مختلف ضدعفونی به‌منظور کشت بافت شمشاد خزری (Buxus hyrcana Pojark.)‌

نوع مقاله : علمی - پژوهشی

نویسندگان

1 چنگلشناسی و اکولوژی چنگل، علوم جنگل، گرگان، دانشگاه علوم منابع طبیعی گرگان

2 دانشگاه علوم کشاورزی و منابع طبیعی گرگان . دانشکده علوم جنگل

3 گروه جنگلشناسی و اکولوژی‌جنگل، دانشگاه علوم کشاورزی و منابع طبیعی گرگان

چکیده

این پژوهش با هدف یافتن بهترین ریزنمونه و فصل نمونه­برداری تحت تأثیر تیمارهای مختلف سترون­سازی برای دست­یابی به دستورالعمل مناسب و بهینه برای پژوهش­های کشت بافت شمشاد خزری انجام شد. ریزنمونه­های برگ و ساقه از بخش­های مختلف گیاه تهیه شدند. ابتدا پیش­­تیمارهای ضدعفونی ریزنمونه­ها و شستشوی اولیه با آب معمولی دارای چند قطره مایع ظرف­شویی به­مدت چهار تا پنج دقیقه به­منظور حذف آلودگی­های سطحی انجام شد. سپس در زیر هود استریل سترون­سازی با استفاده از 13 تیمار مختلف انجام شده و بعد از اعمال هر تیمار، پنج بار با آب مقطر استریل آبشویی و در پایان ریزنمونه­های مناسب از مواد گیاهی جدا و روی محیط کشت PDA در داخل ­پتری­های استریل در کنار شعله قرار داده شدند. برای اندازه­گیری آلودگی، کشت­ها در اتاق رشد با دمایی معادل 1±26 درجه سانتی­گراد با شدت نور 1000 تا 1500 لوکس و تاریکی مورد بررسی قرار گرفتند. نتایج نشان داد ریزنمونه­های فصل بهار نسبت به ریزنمونه­های فصل پاییز دارای آلودگی میکروبی کمتری هستند. از طرفی ریزنمونه­های برگی نسبت به ریزنمونه­های ساقه درصد آلودگی کمتری از خود نشان دادند.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation type of explants and season of sampling under different disinfection treatments for the tissu culture of Buxus hyrcana Pojark

نویسندگان [English]

  • Allahverdi Mohammadzadeh 1
  • vahide payamnoor 2
  • Mohammadreza kavosi 3
1 PhD. Student., Silviculture and Forest Ecology, Dept. of Forest Sciences, Gorgan University of Agricultural Sciences and Natural Recourses. Golestan, Iran
2 Gorgan Unversity of Agricultural Sciences and Natural Resource. forest sciences faculty
3 Dept. of Forest Sciences, Gorgan University of Agricultural Sciences and Natural Recourses. Golestan, Iran
چکیده [English]

The aim of this study was to find the best explants and sampling period under different sterilization treatments in order to achieve a suitable and optimal protocol for tissue culture studies in Buxus sp. Explants were prepared from different parts of the plant, including leaves and stems. At first, detergent disinfection treatments and initial washing with ordinary water containing a few drops of liquid dish for four to five minutes were performed to remove surface contamination. The Explants were flushed with distilled water five times. Then, under laminar Floe sterilization, use 13 different treatments and disinfect five times each treatment with distilled autoclaved leachate and at the end of the appropriate samples (leaf samples of 0.5 to 1 cm Squares and stem samples of 5 to 10 mm) were isolated from plant material and placed on a PDA medium inside autoclaved petri dishes alongside a flame. In order to measure the contamination, cultures in a growth room with a temperature of 26 ± 1 ° C with a light intensity of 1000 to 1500 lux and dark (incubator) were investigated. The results showed that samples of spring season have less microbial contamination than those in the autumn season. On the other hand, leaf samples showed less contamination percentage compared to stem explants.

کلیدواژه‌ها [English]

  • Boxblight
  • Treatments of sterilization
  • Buxus hyrcana Pojark
  • Tissu culture

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پژوهش و توسعه جنگل
دوره 5، شماره 4
دی 1398
صفحه 527-540

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