Proceedings of the 2015 International Conference on Materials Chemistry and Environmental Protection (meep-15)

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Construction and Functional Analysis of Luciferase Reporter Plasmid Containing GSNOR Gene Promoter

Authors
D.L. Zheng, X.H. Liao, Z.Q. Wei, F. Du, H.P. He, T.C. Zhang
Corresponding Author
D.L. Zheng
Available Online March 2016.
DOI
10.2991/meep-15.2016.51How to use a DOI?
Keywords
GSNO; GSNOR; S-nitrosylation; luciferase activity assay
Abstract

S-nitrosoglutathione (GSNO) is a nitrosylating agent which mediates the posttranslational process of Snitrosylation on proteins resulting in modulation of their activity. GSNOR (S-Nitrosoglutathione reductase) is an enzyme related to GSNO degration, which has been used extensively in controlling S-nitrosylation levels in vivo. Myocardin is thought to have a key role in Vascular Smooth Muscle Cell (VSMC) development by acting on CArG-dependent genes. In this study, a human GSNOR promoter luciferase reporter construct was generated by PCR amplification of GSNOR promoter. The PCR fragment was digested and cloned into pGL3 vector. The promoter sequence was verified by sequencing. The results showed that luciferase reporter plasmids of GSNOR promoter were successfully constructed. Then the effects of a key transcription factor, which plays important roles in S-nitrosylation levels control, were investigated by luciferase reporter assays in VSMCs. The results showed that myocardin can enhance transcriptional activity of GSNOR. Our research will help us to screen some novel factors in regulating S-nitrosylation levels in vivo.

Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2015 International Conference on Materials Chemistry and Environmental Protection (meep-15)
Series
Advances in Physics Research
Publication Date
March 2016
ISBN
10.2991/meep-15.2016.51
ISSN
2352-541X
DOI
10.2991/meep-15.2016.51How to use a DOI?
Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - D.L. Zheng
AU  - X.H. Liao
AU  - Z.Q. Wei
AU  - F. Du
AU  - H.P. He
AU  - T.C. Zhang
PY  - 2016/03
DA  - 2016/03
TI  - Construction and Functional Analysis of Luciferase Reporter Plasmid Containing GSNOR Gene Promoter
BT  - Proceedings of the 2015 International Conference on Materials Chemistry and Environmental Protection (meep-15)
PB  - Atlantis Press
SP  - 194
EP  - 197
SN  - 2352-541X
UR  - https://doi.org/10.2991/meep-15.2016.51
DO  - 10.2991/meep-15.2016.51
ID  - Zheng2016/03
ER  -