Olekshiy P. V., Lapovets L. E., Ushtan S. V., Horitsky V. M.

EFFECT OF DENTAL IMPLANATION AND MAGNETOTHERAPY ON THE CITOKINAL STATUS OF PATIENTS


About the author:

Olekshiy P. V., Lapovets L. E., Ushtan S. V., Horitsky V. M.

Heading:

DENTISTRY

Type of article:

Scentific article

Annotation:

Dental implantology in modern conditions is a promising direction of dental science and can provide virtually complete restoration of chewing function at the admission, as well as contributes to restoring the aesthetic appearance of the patient. Difficulties that have arisen in the process of using dental implantation (DI) are primarily due to postoperative complications. In order to avoid the development of the inflammatory process and the emergence of other periodontal diseases, it is important to study the immune status of the subjects undergoing implantation. During the last decades a lot of data has been accumulated that indicates the existence of bioregulatory effects of the electromagnetic field, which testifies the possibility of applying a weak magnetic field to a living organism to enhance the therapeutic effect. The purpose of our work was to study the cytokine profile (IL1β, IL2, IL6 and TNF-α) of the oral fluid and blood serum of individuals who received magnetic therapy (MT) after DI. We conducted a clinical and laboratory examination of 50 persons who had impressions before dental implantation. 25 patients were treated with magnetotherapy: during 6 days for 12 minutes each day, the effect of the low-frequency sinusoidal magnetic field on the dental implantation zone was performed. The average age of patients was from 20 to 55 years. The obtained laboratory parameters were compared with the control group, which included 20 practically healthy persons with a salivated oral cavity. The determination of interleukins was carried out using a set of reagents from Vector Best Company, Ukraine, on the STAT FAX 303 plus analyzer. The parametric data is given as M ± m, since the data distribution in the groups was normal; pairwise a posteriori comparison of the groups was performed using the Newman-Calex criterion using the STATISTICA 6.0 software package (StatSoft, USA). As a result of our researches, we found that in the mouth liquid individuals who, after dental implantation, used magnetic therapy, kept elevated levels of IL-1β, IL-2, IL-6 and TNF-α (57.6 times; 27 times; in 3,45 times, 4 times, respectively) in comparison with the level of healthy subjects, but after the influence of the magnetic field, the content of these cytokines in the oral liquid decreased (1.2 times, in 1.3 times, in 1.1 times, in 1.4 times, respectively) compared to the group where no magnetotherapy was used. In the serum of individuals who, after dental implantation, used magnetotherapy, the content of IL-1β, IL-2 and TNF-α was higher (5.3 times, 25.7 times, 2.3 times) than the content in the control group, but the level of IL-6 remained below the control by 1.3 times. After magnetotherapy, levels of IL-1β, IL-2 and TNF-α in serum decreased (1.18 times, 1.6 times, 1.1 times, respectively) relative to the levels in the group where only dental implantation was performed, only the content of IL-6 increased by 1.5 times to the level in the group without magnetotherapy. Thus, as a result of the study of the dynamic changes in the levels of interleukins in the oral fluid, we discovered that prior to surgery for DI, there was a significant increase in all studied cytokines, after the operation there was a statistically significant decrease in their content, confirming the pathogenetic significance of the studied cytokines in the processes of inflammation and remodeling of bone tissue. The use of magnetotherapy facilitated the approximation of interleukin levels to normal values. According to literature data, the effect of low-frequency magnetic field improves microcirculation in the field of dental implantation, and magnetotherapy is an effective method for the prevention of inflammatory postoperative complications, accelerates the processes of osteointegration and shortens the treatment time of patients.

Tags:

cytokines, oral fluid, serum, implantation, magnetotherapy

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Publication of the article:

«Bulletin of problems biology and medicine» Issue 4 Part 3 (141), 2017 year, 360-363 pages, index UDK 616.314-089.843-085.847.8-083

DOI: