Simulation of prehepatic portal hypertension and impact of hepatic deportalization on its tissue

Keywords: prehepatic portal hypertension, portal vein, rats, partial ligature of vena porta, hepatocyte

Abstract

Objective. On the prehepatic portal hypertension pattern, created by partial ligature of venae portae in rats, to estimate the impact of partial hepatic deportalization on its tissue.

Materials and methods. The investigation was conducted on 35 male rats of a Wistar line, aged 4–6 weeks. In 15 laboratory animals the partial ligature of vena porta with formation of stenosis was conducted, and in 15 –false ligature, while the control group consisted of 5 intact laboratory animals.

Results. There were revealed certain connections between conduction of partial ligature of vena porta and critical (lethal) lowering of portohepatic perfusion.

The model of prehepatic portal hypertension, using partial ligature of vena porta, leading to partial lowering of portohepatic perfusion with development of cavernous transformation of vena porta and dystrophic changes in hepatocytes with further fibrous changes in hepatic parenchyma, was applied.

Conclusion. The portal vein narrowing grade in range 52 – 59% constitutes the optimal one for the prehepatic portal hypertension formation with development of zonal adipose parenchymatous hepatic dystrophy and the balloon degeneration of hepatocytes with further development of fibroses in rats of a Wistar line aged 4 – 6 weeks.

Author Biography

О. S. Godik, Bogomolets National Medical University, Kyiv, National Pediatric Specialized Hospital «OKHMATDYT», Kyiv

Godik Oleh Sviatoslavovych, MD, PhD(Med), assistant professor, pediatric surgeon, transplantologist
Department of Pediatric Surgery
Bogomolets National Medical University, Kyiv, Ukraine
National Children’s Specialized Hospital “Okhmatdyt”, Kyiv, Ukraine
28/1 Chornovola str., 01135, Kyiv, Ukraine
+38(050)5433781
ogodik@gmail.com
https://orcid.org/0000-0002-1084-9484

References

Neuhof H. Experimental ligation of the portal vein; its application to the treatment of suppurative pylephlebitis. Surg Gynecol Obstet. [Internet]. 1913;16:481-3. [cited Milliken NT, Stryker HB. Suppurative Pylethrombophlebitis and Multiple Liver Abscesses Following Acute Appendicitis. New England Journal of Medicine. 1951;244(2):52–4. doi:10.1056/nejm195101112440203].

Reynell PC. Portal hypertension in the rat. Br J Exp Pathol [Internet] 1952 Feb; 33:19-24. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2073224/.

Myking AO, Halvorsen JF. A method for graded portal vein stenosis in rats: survival related to degree of stenosis. Eur Surg Res. 1973;5(6):454-7. doi: 10.1159/000127689. PMID: 4792412.

Myking AO, Halvorsen JF. Two-stage occulsion of the protal vein in the rat:survival related to weight variation and the interval between partial and total occulusion. Eur Surg Res. 1975;7(6):366-74. doi: 10.1159/000127821. PMID: 1237406.

Laufman H, Bernhard V, Roach HD, Champlain G. Experimental production of esophageal varices in the Macaca rhesus. Surg Gynecol Obstet. 1960 Apr;110:451-6. PMID: 14414515.

Jensen LS, Krarup N, Larsen JA, Juhl C, Nielsen TH, Dybdahl H. Chronic portal venous hypertension. The effect on liver blood flow and liver function and the development of esophageal varices. Scand J Gastroenterol. 1987 May;22(4):463-70. doi: 10.3109/00365528708991492. PMID: 3299679.

Jensen LS, Dybdahl H, Juhl C. Experimental prehepatic portal hypertension and esophageal varices in the rabbit. Scand J Gastroenterol. 1986 Mar;21(2):225-30. doi: 10.3109/00365528609034651. PMID: 3715389.

Cheever AW, Warren KS. Portal vein ligation in mice: portal hypertension, collateral circulation, and blood flow. J Appl Physiol. 1963 Mar;18:405-7. doi: 10.1152/jappl.1963.18.2.405. PMID: 14020470.

Wen Z, Zhang JZ, Xia HM, Yang CX, Chen YJ. Stability of a rat model of prehepatic portal hypertension caused by partial ligation of the portal vein. World J Gastroenterol. 2009 Aug 28;15(32):4049-54. doi: 10.3748/wjg.15.4049. PMID: 19705502; PMCID: PMC2731957.

Rodrigues DA, da Silva AR, Serigiolle LC, Fidalgo Rde S, Favero SS, Leme PL. Constriction rate variation produced by partial ligation of the portal vein at pre-hepatic portal hypertension induced in rats. Arq Bras Cir Dig. 2014 Nov-Dec;27(4):280-4. doi: 10.1590/S0102-67202014000400012. PMID: 25626939; PMCID: PMC4743222.

Law of Ukraine «On protection of animals from cruelty». [Internet]. 2012; 5456-VI (5456-17). Available from:

https://www.globalanimallaw.org/downloads/database/national/ukraine/library64.pdf.

European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasbourg. 1986; 48. Available from:

https://rm.coe.int/168007a67b.

World Medical Association. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA. 2013 Nov 27;310(20):2191-4. doi: 10.1001/jama.2013.281053. PMID: 24141714.

Petrie A, Sabin C. Medical Statistics at a Glance. 4th ed. New York: Wiley; 2019. 208 p. ISBN: 978-1-119-16783-9.

Rozga J, Jeppsson B, Hägerstrand I, Bengmark S. Acute portal vein stenosis. An experimental study on portal circulation and hepatosplenic function. Acta Chir Scand. 1985;151(2):125-31. PMID: 4002975.

Halvorsen JF, Myking AO. Prehepatic portal hypertension in the rat. Immediate and long-term effects on portal vein and aortic pressure of a graded portal vein stenosis, followed by occlusion of the portal vein and spleno-renal collaterals. Eur Surg Res. 1979;11(2):89-98. doi: 10.1159/000128055. PMID: 488151.

Abraldes JG, Pasarín M, García-Pagán JC. Animal models of portal hypertension. World J Gastroenterol. 2006 Nov 7;12(41):6577-84. doi: 10.3748/wjg.v12.i41.6577. PMID: 17075968; PMCID: PMC4125660.

Wang CT, Kuang YL, Chen ZP. Hyperdynamic status in a partial portal vein ligated (PVL) rat’s portal hypertension model. Zhonghua Wai Ke Za Zhi. 1994 Sep;32(9):573-5. Chinese. PMID: 7720436.

Silva AR, Kriguer-Júnior RJ, Serigiolle LC, Gomes HM, Rodrigues DA, Leme PL. Increase in spleen volume of rats on experimental model of pre-hepatic portal hypertension. Arq Bras Cir Dig. 2013 Jul-Sep;26(3):206-12. English, Portuguese. doi: 10.1590/s0102-67202013000300010. PMID: 24190379.

Lutsyk OD, Chaikovskyi YuB. Histology Cytology Embryology: Textbook. 1st ed. Vinnytsia: New Book; 2018. 592 p. Ukrainian. ISBN: 978-966-382-698-1.

Iancu TC, Manov I. Electron microscopy of liver biopsies. In: Takahashi H. Liver Biopsy. Intech Open Access Publisher; 2011. 109–136. ISBN: 978-953-307-644-7.

Ahishali E, Demir K, Ahishali B, Akyuz F, Pinarbasi B, Poturoglu S, et al. Electron microscopic findings in non-alcoholic fatty liver disease: is there a difference between hepatosteatosis and steatohepatitis? J Gastroenterol Hepatol. 2010 Mar;25(3):619-26. doi:

Lo RC, Kim H. Histopathological evaluation of liver fibrosis and cirrhosis regression. Clin Mol Hepatol. 2017 Dec;23(4):302-7. doi: 10.3350/cmh.2017.0078. Epub 2017 Dec 20. PMID: 29281870; PMCID: PMC5760001.

Published
2022-12-23
Section
Experimental Investigations