In this study, we explained the phytohormone profiles associated with various stages of melon fruit growth. This is achieved using melon fruits were obtained 14 to 56 days after flowering (DAF) and grouped into the rind, pulp, and placenta part (including seeds). The peduncle part (including the petiole of about 10 cm in length) was also obtained. Furthermore, using liquid chromatography-mass spectrometry (LC-MS), we quantified simultaneously indole-3-acetic acid (IAA), abscisic acid (ABA), trans zeatin (tZ), isopentenyladenine (iP), jasmonic acid (JA), methyl jasmonate (MeJA), gibberellin₁ (GA₁) and gibberellin₄ (GA₄) obtained from various tissues. tZ and iP concentrations increased at 42 DAF in the placenta part but rapidly declined at 56 DAF. The GA₄ concentration was similar in the placenta part, the rind, and flesh within the fruit, but changed differently in the peduncle part outside the fruit, rapidly increasing to 28 DAF and continuing to decrease thereafter. ABA concentration in the placenta part continued to increase with the progress of fruit growth. In the rind, the concentrations of JA and MJA were generally high. In conclusion, our results revealed an endogenous plant hormone profile in grape fruit growth.