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Bacterial sucrose isomerases: properties and structural studies

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Abstract

Due to their significant role in food industry, sucrose isomerases are good candidates for rational protein engineering. Hence, specific modifications in order to modify substrate affinity and selectivity, product specificity but also to adapt their catalytic properties to particular industrial process conditions, is interesting. Our work on the structural studies of the sucrose isomerase, MutB, which presents the first structural data available on a trehalulose synthase and the first experimental data on complexed forms of sucrose isomerases represents a significant advance in the understanding of these enzymes. In this review we give an overview of what is known on biochemical properties and structural aspects of different sucrose isomerases in particular those reported from bacteria.

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Authors and Affiliations

  1. Laboratoire de BioCristallographie, Institut de Biologie et Chimie des Protéines, UMR 5086-CNRS/Université de Lyon, IFR128 “BioSciences Gerland — Lyon Sud”, 7 Passage du Vercors, F-69367, Lyon cedex 07, France

    Moez Rhimi, Richard Haser & Nushin Aghajari

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  1. Moez Rhimi
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  2. Richard Haser
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  3. Nushin Aghajari
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Correspondence to Nushin Aghajari.

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Rhimi, M., Haser, R. & Aghajari, N. Bacterial sucrose isomerases: properties and structural studies. Biologia 63, 1020–1027 (2008). https://doi.org/10.2478/s11756-008-0166-0

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