Abstract
Phenolic acids and derivatives of quercetin in Indian (amla) and European gooseberry were determined by high-performance liquid chromatography with diode array detector. The calibration curves were constructed using phenolic compounds standards (the coefficient of determination (R 2) was 0.9990–0.9997 for phenolic acids and 0.9989–0.9994 for flavonols, respectively). The lowest detection limit was 0.28 mg L−1 and 0.35 mg L−1 for hyperoside and gallic acid, respectively, whereas the highest was 1.80 mg L−1 and 7.98 mg L−1 for quercetin and chlorogenic acid, respectively. The quantification limits calculated were 0.85–24.04 mg L−1 for hyperoside and chlorogenic acid, respectively. The predominant phenolic acid in amla and gooseberry is gallic acid: (5.37 ± 0.04) mg per 100 g of dry mass (d.m.) and (3.21 ± 0.03) mg per 100 g of d.m., respectively. The next one was caffeic acid, 0.65–1.22 mg per 100 g of d.m., followed by p-coumaric acid, 0.84–1.17 mg per 100 g of d.m. Out of the flavonols, rutin is predominant: (3.11 ± 0.13) mg per 100 g of d.m. and (2.12 ± 0.03) mg per 100 g of d.m., respectively. Anti-oxidant activity was also determined.
[1] Benzie, I. F. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239, 70–76. DOI: 10.1006/abio.1996.0292. http://dx.doi.org/10.1006/abio.1996.029210.1006/abio.1996.0292Search in Google Scholar
[2] Bhattacharya, A., Chatterjee, A., Ghosal, S., & Bhattacharya, S. K. (1999). Antioxidant activity of active tannoid principles of Emblica officinalis (amla). Indian Journal of Experimental Biology, 37, 676–680. Search in Google Scholar
[3] Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT — Food Science and Technology, 28, 25–30. DOI: 10.1016/s0023-6438(95)80008-5. http://dx.doi.org/10.1016/S0023-6438(95)80008-510.1016/S0023-6438(95)80008-5Search in Google Scholar
[4] British Pharmacopoeia Commission (2005). British Pharmacopoeia. London, UK: The Stationery Office. Search in Google Scholar
[5] de Ancos, B., González, E. M., & Pilar Cano, M. (2000). Ellagic acid, vitamin C, and total phenolic contents and radical scavenging capacity affected by freezing and frozen storage in raspberry fruit. Journal of Agricultural and Food Chemistry, 48, 4565–4570. DOI: 10.1021/jf0001684. http://dx.doi.org/10.1021/jf000168410.1021/jf0001684Search in Google Scholar
[6] Deighton, N., Brennan, R., Finn, C., & Davies, H. V. (2000). Antioxidant properties of domesticated and wild Rubus species. Journal of the Science of Food and Agriculture, 80, 1307–1313. DOI: 10.1002/1097-0010(200007)80:9〈1307:: AID-JSFA638〉3.0.CO;2-P. http://dx.doi.org/10.1002/1097-0010(200007)80:9<1307::AID-JSFA638>3.0.CO;2-P10.1002/1097-0010(200007)80:9<1307::AID-JSFA638>3.0.CO;2-PSearch in Google Scholar
[7] Farmakopea Polska. (2002). Farmakopea Polska (Vol VI). Warszawa, Poland: Polskie Towarzystwo Farmaceutyczne. Search in Google Scholar
[8] Floegel, A., Kim, D. O., Chung, S. J., Koo, S. I., & Chun, O. K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of Food Composition and Analysis, 24, 1043–1048. DOI: 10.1016/j.jfca.2011.01.008. http://dx.doi.org/10.1016/j.jfca.2011.01.00810.1016/j.jfca.2011.01.008Search in Google Scholar
[9] Jain, S. K., & Khurdiya, D. S. (2004). Vitamin C enrichment of fruit juice based ready-to-serve beverages through blending of Indian Gooseberry (Emblica officinalis Gaertn.) juice. Plant Foods for Human Nutrition, 59, 63–66. DOI: 10.1007/s11130-004-0019-0. http://dx.doi.org/10.1007/s11130-004-0019-010.1007/s11130-004-0019-0Search in Google Scholar
[10] Jose, J. K., Kuttan, G., & Kuttan, R. (2001). Antitumour activity of Emblica officinalis. Journal of Ethnopharmacology, 75, 65–69. DOI: 10.1016/s0378-8741(00)00378-0. http://dx.doi.org/10.1016/S0378-8741(00)00378-010.1016/S0378-8741(00)00378-0Search in Google Scholar
[11] Khan, M. T. H., Lampronti, I., Martello, D., Bianchi, N., Jabbar, S., & Choudhuri, M. S. K. (2002). Identification of pyrogallol as an antiproliferative compound present in extracts from the medicinal plant Emblica officinalis: Effect on in vitro cell growth of human tumor cell lines. International Journal of Oncology, 20, 187–192. 10.3892/ijo.21.1.187Search in Google Scholar
[12] Kim, D. O., Lee, K.W., Lee, H. J., & Lee, C. Y. (2002). Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. Journal of Agricultural and Food Chemistry, 50, 3713–3717. DOI: 10.1021/jf020071c. http://dx.doi.org/10.1021/jf020071c10.1021/jf020071cSearch in Google Scholar
[13] Kohlmünzer, S. (2007). Farmakognozja. Warszawa, Poland: Wydawnictwo Lekarskie PZWL. Search in Google Scholar
[14] Kumar, G. S., Nayaka, H., Dharmesh, S. M., & Salimath, P. V. (2006). Free and bound phenolic antioxidant in amla (Emblica officinalis) and turmeric (Curcuma longa). Journal of Food Composition and Analysis, 19, 446–452. DOI: 10.1016/j.jfca.2005.12.015. http://dx.doi.org/10.1016/j.jfca.2005.12.01510.1016/j.jfca.2005.12.015Search in Google Scholar
[15] Pantelidis, G. E., Vasilakakis, M., Manganaris, G. A., & Diamantidis, G. (2007). Antioxidant capacity, phenol, anthocyanin and ascorbic acid contents in raspberries, blackberries, red currants, gooseberries and Cornelian cherries. Food Chemistry, 102, 777–783. DOI: 10.1016/j.foodchem.2006.06.021. http://dx.doi.org/10.1016/j.foodchem.2006.06.02110.1016/j.foodchem.2006.06.021Search in Google Scholar
[16] Perianayagam, J. B., Sharma, S. K., Joseph, A., & Christina, A. J. M. (2004). Evaluation of anti-pyretic and analgesic activity of Emblica officinalis Gaertn. Journal of Ethnopharmacology, 95, 83–85. DOI: 10.1016/j.jep.2004.06.020. http://dx.doi.org/10.1016/j.jep.2004.06.02010.1016/j.jep.2004.06.020Search in Google Scholar
[17] Polasa, K., Sesikaran, B., Krishna, T. P., & Krishnaswamy, K. (1991). Turmeric (Curcuma longa)-induced reduction in urinary mutagens. Food and Chemical Toxicology, 29, 699–706. DOI: 10.1016/0278-6915(91)90128-t. http://dx.doi.org/10.1016/0278-6915(91)90128-T10.1016/0278-6915(91)90128-TSearch in Google Scholar
[18] Pornpimon, M., & Sakamon, D. (2008). Antimicrobial and antioxidant activities of Indian gooseberry and galangal extracts. LWT — Food Science and Technology, 41, 1153–1159. DOI: 10.1016/j.lwt.2007.07.019. http://dx.doi.org/10.1016/j.lwt.2007.07.01910.1016/j.lwt.2007.07.019Search in Google Scholar
[19] Prior, R. L., Cao, G., Martin, A., Sofic, E., McEwan, J., O’Brien, C., Lischner, N., Ehlenfeldt, M., Kalt, W., Krewer, G., & Mainland, M. (1998). Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity and variety of Vaccinium species. Journal of Agricultural and Food Chemistry, 46, 2686–2693. DOI: 10.1021/jf980145d. http://dx.doi.org/10.1021/jf980145d10.1021/jf980145dSearch in Google Scholar
[20] Raghu, V., Platel, K., & Srinivasan, K. (2007). Comparison of ascorbic acid content of Emblica officinalis fruits determined by different analytical methods. Journal of Food Composition and Analysis, 20, 529–533. DOI: 10.1016/j.jfca.2007.02.006. http://dx.doi.org/10.1016/j.jfca.2007.02.00610.1016/j.jfca.2007.02.006Search in Google Scholar
[21] Sharma, N., Trikhaa, P., Athar, M., & Raisuddin, S. (2000). In vitro inhibition of carcinogen-induced mutagenecity by Cassia occidentalis and Emblica officinalis. Drug and Chemical Toxicology, 23, 477–484. DOI: 10.1081/dct-100100129. http://dx.doi.org/10.1081/DCT-10010012910.1081/DCT-100100129Search in Google Scholar
[22] Singleton, V. L., & Rossi, J. A., Jr. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144–158. Search in Google Scholar
[23] Thakur, C. P., Thakur, B., Singh, S., Sinha, P. K., & Sinha, S. K. (1988). The Ayurvedic medicines, Haritaki, Amla and Bahira reduce cholesterol-induced atherosclerosis in rabbits. Indian Journal of Cardiology, 21, 167–172. DOI: 10.1016/0167-5273(88)90219-7. http://dx.doi.org/10.1016/0167-5273(88)90219-710.1016/0167-5273(88)90219-7Search in Google Scholar
[24] Wang, S. Y., & Lin, H. S. (2000). Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. Journal of Agricultural and Food Chemistry, 48, 140–146. DOI: 10.1021/jf9908345. http://dx.doi.org/10.1021/jf990834510.1021/jf9908345Search in Google Scholar PubMed
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