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Redescription and First Nucleotide Sequences of Rhinobatonchocotyle pacifica Oliva & Luque, 1995 (Monogenea: Hexabothriidae), a Parasite of Pseudobatos planiceps (Garman, 1880) (Rhinopristiformes: Rhinobatidae) from Peru

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Abstract

Introduction

Specimens of Rhinobatonchocotyle pacifica Oliva & Luque, 1995 (Hexabothriidae) were collected from the gill filaments of Pacific guitarfish, Pseudobatos planiceps (Garman, 1880) (Rhinopristiformes: Rhinobatidae), from off the coast of Chorrillos, Lima, Peru. Since the original description of R. pacifica was incomplete, we herein redescribe R. pacifica based on re-examination of the holotype, paratypes, and newly collected voucher specimens. Nucleotide sequences (18S, 28S) for R. pacifica are presented for the first time, and a phylogenetic analysis conducted.

Materials and Methods

Monogeneans were examined with light microscopy and partial sequences of 18S and 28S rDNA genes were obtained.

Results

The following taxonomically important morphological features are described: presence of seminal receptacle, spiniform papillae within oral cavity, haptoral sucker sclerites and anchors, hook surface striations, muscular pad associated with each haptoral sucker sclerite (except sclerite 1), muscular bulbs in appendix suckers, and oötype lacking longitudinal rows of large cells (oötype not côtelé). The genital complex is detailed. The diagnosis of Rhinobatonchocotyle is emended to include species with or without seminal receptacle. Representative nucleotide sequences of partial 18S and 28S rDNA genes of R. pacifica were obtained for the first time and used for reconstructing the phylogeny of Hexabothriidae.

Conclusion

The phylogenetic reconstruction confirmed the species within the Hexabothriidae.

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Acknowledgements

To Anna Phillips (USNM) for allowing us access to specimens under her care. We also thank Griselda Pulido-Flores for providing us with valuable literature on hexabothriids. The authors are grateful to the following people who helped to the collection of fishes in Peru: Ivette Cuellar, Eva G. Huancachoque and Cynthia E. Rodríguez, all from the National University Federico Villarreal (UNFV). José L. Luque and Cláudia P. Santos were supported by a Researcher fellowship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (CNPq). Jhon D. Chero and Celso L. Cruces were supported by a student fellowship from the Coordenação de Aperfeiçoamento de Pessoal do Ensino Superior, Brazil (CAPES)—Finance Code 001.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Biologia Animal da Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro, CEP 23890-000, Brazil

    Jhon D. Chero

  2. Programa de Pós-Graduação em Ciências Veterinárias da Universidade Federal Rural do Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro, CEP 23890-000, Brazil

    Celso L. Cruces

  3. Laboratorio de Parasitología General y Especializada, Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal (UNFV), El Agustino, Código postal 15007, Lima, Peru

    Jhon D. Chero, Celso L. Cruces & Gloria Sáez

  4. Laboratório de Avaliação e Promoção da Saúde Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, RJ, CEP 21040-360, Brazil

    Ana C. A. Camargo & Cláudia Portes Santos

  5. Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Caixa postal 74.540, Seropédica, Rio de Janeiro, CEP 23851-970, Brazil

    José L. Luque

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  1. Jhon D. Chero
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  2. Celso L. Cruces
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  3. Gloria Sáez
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Chero, J.D., Cruces, C.L., Sáez, G. et al. Redescription and First Nucleotide Sequences of Rhinobatonchocotyle pacifica Oliva & Luque, 1995 (Monogenea: Hexabothriidae), a Parasite of Pseudobatos planiceps (Garman, 1880) (Rhinopristiformes: Rhinobatidae) from Peru. Acta Parasit. 64, 797–806 (2019). https://doi.org/10.2478/s11686-019-00101-4

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