Abstract
The present work describes the development of a nanocomposite system and its application in construction of a new amperometric biosensor applied in the determination of total polyphenolic content from propolis extracts. The nanocomposite system was based on covalent immobilization of laccase on functionalized indium tin oxide nanoparticles and it was morphologically and structural characterized. The casting of the developed nanocomposite system on the surface of a screen-printed electrode was used for biosensor fabrication. The analytical performance characteristics of the settled biosensor were determined for rosmarinic acid, caffeic acid and catechol (as laccase specific substrate). The linearity was obtained in the range of 1.06×10−6 − 1.50×10−5 mol L−1 for rosmarinic acid, 1.90×10−7 − 2.80×10−6 mol L−1 for caffeic acid and 1.66×10−6 − 7.00×10−6 mol L−1 for catechol. A good sensitivity of amperometric biosensor 141.15 nA µmol−1 L−1 and fair detection limit 7.08×10−8 mol L−1 were obtained for caffeic acid. The results obtained for polyphenolic content of propolis extracts were compared with the chromatographic data obtained by liquid-chromatography with diode array detection. 
[1] X. Feng, R. Li, Y. Ma, Q. Fan, W. Huang, Synthetic Met. 161, 1940 (2011) http://dx.doi.org/10.1016/j.synthmet.2011.06.03910.1016/j.synthmet.2011.06.039Search in Google Scholar
[2] Z. Gao, Y.Z. Gao, Y.H. Li, L. Yi, Nanostruct. Mater. 11, 611 (1999) 10.1016/S0965-9773(99)00347-5Search in Google Scholar
[3] C. Goebbert, R. Nonninger, M.A. Aegerter, H. Scmidt, Thin Solid Films 351, 79 (1999) 10.1016/S0040-6090(99)00209-6Search in Google Scholar
[4] A. Radoi, A. Iordanescu, A. Cismaru, M. Dragoman, D. Dragoman, Nanotechnology 21, 455202 (2010) http://dx.doi.org/10.1088/0957-4484/21/45/45520210.1088/0957-4484/21/45/455202Search in Google Scholar PubMed
[5] V. Senthilkumar, K. Senthil, P. Vickraman, Mater. Res. Bull. 47, 1051 (2012) http://dx.doi.org/10.1016/j.materresbull.2011.12.04010.1016/j.materresbull.2011.12.040Search in Google Scholar
[6] A.I. Yaropolov, O.V. Skorobogat’ko, S.S. Vartanov, S.D. Varfolomeyer, Appl. Biochem. Biotech. 49, 257 (1994) 10.1007/BF02783061Search in Google Scholar
[7] C.F. Thurston, Microbiology 140, 19 (1994) http://dx.doi.org/10.1099/13500872-140-1-1910.1099/13500872-140-1-19Search in Google Scholar
[8] P. V. Bernhard, Aust. J. Chem. 59, 233 (2006) http://dx.doi.org/10.1071/CH0534010.1071/CH05340Search in Google Scholar
[9] P. J. Collins, M. Kotterman, J. A. Field, A. Dobson, Appl. Environ. Microbiol. 62, 4563 (1996) 10.1128/aem.62.12.4563-4567.1996Search in Google Scholar PubMed PubMed Central
[10] M. Y. Ahn, J. Dec, J. E. Kim, J. M. Bollag, J. Environ. Qual. 31, 1509 (2002) http://dx.doi.org/10.2134/jeq2002.150910.2134/jeq2002.1509Search in Google Scholar PubMed
[11] F. Vianello, S. Ragusa, M.T. Cambria, A. Rigo, Biosens. Bioelectron. 21, 2155 (2006) http://dx.doi.org/10.1016/j.bios.2005.10.00610.1016/j.bios.2005.10.006Search in Google Scholar
[12] S.A.S.S Gomes, M.J.F. Rebelo, Sensors 3, 166 (2003) http://dx.doi.org/10.3390/s3060016610.3390/s30600166Search in Google Scholar
[13] A. E. Gardiol, R. J. Hernandez, B. Reinhammar, B. R. Harte, Enz. Microb. Technol. 18, 347 (1996) 10.1016/0141-0229(95)00110-7Search in Google Scholar
[14] D. Leech, F. Daigle, Analyst 123, 19714 (1998) 10.1039/a803313gSearch in Google Scholar
[15] C.A. Rice-Evans, N.J. Miller, G. Paganga, Trends Plant Sci. 2, 152 (1997) 10.1016/S1360-1385(97)01018-2Search in Google Scholar
[16] A. Salvi, C. Brihlmann, E. Migliavacca, P.A. Carrupt, K. Hostettmann, B. Testa, Helv. Chim. Acta 85 (2002) 10.1002/1522-2675(200203)85:3<867::AID-HLCA867>3.0.CO;2-ZSearch in Google Scholar
[17] M. Antolovich, P.D. Prenzler, E. Patsalides, S. McDonald, K. Robards, Analyst, 127, 183 (2002) http://dx.doi.org/10.1039/b009171p10.1039/b009171pSearch in Google Scholar
[18] V.S. Bankova, S.L. Castro, M.C. Marcucci, Apidologie 31, 3 (2000) http://dx.doi.org/10.1051/apido:200010210.1051/apido:2000102Search in Google Scholar
[19] J.B. Daleprane, V. da Silva Freitas, A. Pacheco, M. Rudnicki, L.A. Faine, F.A. Dörr, M. Ikegaki, L.A. Salazar, T.P. Ong, D.S. Abdalla, J. Nutr. Biochem. 23, 557 (2012) http://dx.doi.org/10.1016/j.jnutbio.2011.02.01210.1016/j.jnutbio.2011.02.012Search in Google Scholar
[20] E.W. Teixeira, G. Negri, R.M.S.A. Meira, D. Message, A. Salatino, Evidence Based Complementary Alternative Medicine 2, 85 (2005) http://dx.doi.org/10.1093/ecam/neh05510.1093/ecam/neh055Search in Google Scholar
[21] C. Castaldo, F. Capasso, Fitoterapia 73, 1 (2002) http://dx.doi.org/10.1016/S0367-326X(02)00185-510.1016/S0367-326X(02)00185-5Search in Google Scholar
[22] M. González, B. Guzmán, R. Rudyk, E. Romano, M.A.A. Molina, Lat. Am. J. Pharm. 22, 243 (2003) Search in Google Scholar
[23] A.C.H.F. Sawaya, I.B. da Silva Cunha, M.C Marcucci, Chem. Cent. J. 5, 27 (2011) http://dx.doi.org/10.1186/1752-153X-5-2710.1186/1752-153X-5-27Search in Google Scholar
[24] S.A.S.S. Gomes, J.M.F. Nogueira, M.J.F. Rebelo, Biosens. Bioelectron. 20, 1211 (2004) http://dx.doi.org/10.1016/j.bios.2004.05.01310.1016/j.bios.2004.05.013Search in Google Scholar
[25] D.R. Theâvenot, K. Toth, R.A. Durst, G.S. Wilson, Pure Appl. Chem. 71, 2333 (1999) 10.1351/pac199971122333Search in Google Scholar
[26] Y. Pang, Z. Guang-Ming, T. Lin, Z. Yi, Li Zhen, C. Li-Juan, J. Cent. South Univ. Technol. 18, 1849 (2011) http://dx.doi.org/10.1007/s11771-011-0913-110.1007/s11771-011-0913-1Search in Google Scholar
[27] R.S. Freire, N. Durán, L.T. Kubota, Talanta 54, 681 (2001) http://dx.doi.org/10.1016/S0039-9140(01)00318-610.1016/S0039-9140(01)00318-6Search in Google Scholar
[28] J. Kulys, R. Vidziunaite, Biosens Bioelectron. 18, 319 (2003) http://dx.doi.org/10.1016/S0956-5663(02)00172-010.1016/S0956-5663(02)00172-0Search in Google Scholar
[29] G. Gupta, V. Rajendran, P. Atanassov, Electroanalysis 15, 1577 (2003) http://dx.doi.org/10.1002/elan.20030272410.1002/elan.200302724Search in Google Scholar
[30] D. Quan, Y. Kim, W. Shin, J. Electroanal. Chem. 561, 181 (2004) http://dx.doi.org/10.1016/j.jelechem.2003.08.00310.1016/j.jelechem.2003.08.003Search in Google Scholar
[31] J. Zou, S.M. Kauzlarich, J. Clust. Sci., 19, 341 (2008) http://dx.doi.org/10.1007/s10876-008-0182-910.1007/s10876-008-0182-9Search in Google Scholar
[32] J.W. Schoonen, M.G. Sales, Anal. Bioanal. Chem. 372, 822 (2002) http://dx.doi.org/10.1007/s00216-002-1267-110.1007/s00216-002-1267-1Search in Google Scholar
[33] C.L. Nistor, D. Donescu, R. Ianchis, C. Spataru, V. Raditoiu, C. Petcu, M. Ghiurea, C. Deleanu, J. Sol-Gel Sci.Techn 59, 48 (2011) http://dx.doi.org/10.1007/s10971-011-2461-410.1007/s10971-011-2461-4Search in Google Scholar
[34] E. Teodor, S.C. Liţescu, C. Petcu, M. Mihalache, R. Somoghi, Nanoscale Res. Lett. 4, 544 (2009) http://dx.doi.org/10.1007/s11671-009-9278-x10.1007/s11671-009-9278-xSearch in Google Scholar
[35] M. Diaconu, A. Tache, S.A.V. Eremia, F. Gatea, S. Litescu, G.L. Radu, U.P.B. Sci. Bull. 72, 115 (2010) Search in Google Scholar
[36] H. Yi, K. Wu, S. Hu, D. Cui, Talanta 55, 1205 (2001) http://dx.doi.org/10.1016/S0039-9140(01)00531-810.1016/S0039-9140(01)00531-8Search in Google Scholar
[37] A. Jarosz-Wilkołazka, T. Ruzgas, L. Gorton, Talanta 66, 1219 (2005) http://dx.doi.org/10.1016/j.talanta.2005.01.02610.1016/j.talanta.2005.01.026Search in Google Scholar PubMed
[38] M. ElKaoutit, I. Naranjo-Rodriguez, K.R. Temsamani, M.P. Hernández-Artiga, D. Bellido-Milla, J.L.H.H.D. Cisneros, Food Chem. 110, 1019 (2008) http://dx.doi.org/10.1016/j.foodchem.2008.03.00610.1016/j.foodchem.2008.03.006Search in Google Scholar PubMed
[39] G. Socrates, Infrared and Raman Characteristic Group Frequencies, 3rd edition (John Wiley & Sons Ltd, Cichester, England, 2001) 328 Search in Google Scholar
[40] S.K. Arya, A.K. Prusty, S.P. Singh, P.R. Solanki, M.K. Pandey, M. Datta, B.D. Malhotra, Anal. Biochem. 363, 210 (2007) http://dx.doi.org/10.1016/j.ab.2007.01.02910.1016/j.ab.2007.01.029Search in Google Scholar PubMed
[41] M. Lorenzo, D. Moldes, S. Rodríguez Couto, M.A. Sanromán, Chemosphere, 60, 1124 (2005) http://dx.doi.org/10.1016/j.chemosphere.2004.12.05110.1016/j.chemosphere.2004.12.051Search in Google Scholar PubMed
[42] Y.Z. Xiao, X.M. Tu, J. Wang, M. Zhang, Q. Cheng, W.Y. Zeng, Y.Y. Shi, Appl. Microbiol. Biotechnol. 60, 700 (2003) 10.1007/s00253-002-1169-3Search in Google Scholar PubMed
[43] V.C. Sanz, M.L. Mena, A. Gonzalez-Cortes, P. Yanez-Sedeno, J.M. Pingarron, Anal.Chim. Acta 528, 1 (2005) http://dx.doi.org/10.1016/j.aca.2004.10.00710.1016/j.aca.2004.10.007Search in Google Scholar
[44] M. Diaconu, S.C. Litescu, G.L. Radu, Sensors and Actuators B, 145, 800 (2010) http://dx.doi.org/10.1016/j.snb.2010.01.06410.1016/j.snb.2010.01.064Search in Google Scholar
[45] P. Ibarra-Escutia, J.J. Gomez, C. Calas-Blanchard, J.L. Marty, M.T. Ramirez-Silva, Talanta 81, 1636 (2010) http://dx.doi.org/10.1016/j.talanta.2010.03.01710.1016/j.talanta.2010.03.017Search in Google Scholar
[46] M. Diaconu, S.C. Litescu, G.L. Radu, Microchim. Acta 172, 177 (2011) http://dx.doi.org/10.1007/s00604-010-0486-y10.1007/s00604-010-0486-ySearch in Google Scholar
[47] S.C. Litescu, S.A.V. Eremia, A. Bertoli, L. Pistelli, G.L. Radu, Anal. Lett. 43, 1089 (2010) http://dx.doi.org/10.1080/0003271090351851810.1080/00032710903518518Search in Google Scholar
[48] A.C.H.F. Sawaya, I.B.S. Cunha, M.C Marcucci, Chem. Cent. J. 5, 27 (2011) http://dx.doi.org/10.1186/1752-153X-5-2710.1186/1752-153X-5-27Search in Google Scholar
[49] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, Free Rad. Biol. Med. 26, 1231 (1999) http://dx.doi.org/10.1016/S0891-5849(98)00315-310.1016/S0891-5849(98)00315-3Search in Google Scholar
[50] G. Litwinienko, K.U. Ingold, J. Org. Chem. 69, 5888 (2004) http://dx.doi.org/10.1021/jo049254j10.1021/jo049254jSearch in Google Scholar PubMed
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