Author(s)

Zebin Chen, Xi Gao, Li Lin, Yuan Su, Jiani Liu, Lei Yu, Dingkang Wang, Shengguang Xu

Corresponding Author

Shengguang Xu

ABSTRACT

The aim of this paper is to study the microbial diversity of rhizosphere soil of tobacco. The research objects are rhizosphere soil samples of four tobacco varieties, Yunyan 85, Yunyan 87, Yunyan 202 and K326. High throughput sequencing technique is used to sequence DNA genome in 16S rDNA-V4 and ITS1 regions of rhizosphere soil of four tobacco varieties; the species abundance, Alpha diversity and Beta diversity are analyzed. The results show that the sequence of bacterial diversity from high to low was Yunyan 202, Yunyan 87, K326 and Yunyan 85; the order of fungal diversity from high to low was Yunyan 202, K326, Yunyan 87 and Yunyan 85. Bacteria were mainly distributed in Proteobacteria and Actinobacteria. The dominant bacteria were Kaistobacter and Rhodanobacter. Fungi were mainly distributed in 5 phyla, include Ascomycota and Zygomycota. The dominant fungi were Pseudogymnoascus. The rhizosphere soil of Yunyan 202 had Pseudomonas, which was not found in the soil of other 3 species. Marinobacter and Alcanivorax were abundant in Yunyan 202 and Yunyan 85, but extremely rare in other 2 species. Pseudonocardia, Janthinobacterium, Sphingomonas, Burkholderia and Rhodanobacter could be found in the soil samples of all species, of which the abundances of Janthinobacterium, Sphingomonas and Burkholderia varied greatly, while the abundances of Pseudonocardia and Rhodanobacter differed slightly. For the fungi diversity, Retroconis and Tremellomycetes sp could only be found in Yunyan 202. The abundances of Penicillium and Sordariomycetes SP were similar in Yunyan 202 and Yunyan 85; in the other two varieties almost no Penicillium or Sordariomycetes SP were detected. Fungi of Myrothecium, Chaetomium, Fungi SP and Pseudogymnoascus could be found in the soil of all species, but their abundances differed greatly. For instance, the abundances of Myrothecium were 1.73% in Yunyan 202, 2.06% in Yunyan 87, 1.44% in Yunyan 85 and 3.76% in K326.

KEYWORDS

High throughput sequencing, varieties, tobacco, rhizosphere soil, microorganism