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Journal of the Serbian Chemical Society 2011 Volume 76, Issue 11, Pages: 1485-1496
https://doi.org/10.2298/JSC101111130S
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Phenolic content and antioxidant activity of Phymatopteris hastata

Su Wei (College of Chemistry and Life Science, Guangxi Teachers Education University, Nanning, China)
Li Peiyuan (College of Pharmacy, Guangxi Traditional Chinese Medical University, Nanning, China)
Huo Lini (College of Pharmacy, Guangxi Traditional Chinese Medical University, Nanning, China)
Wu Caiying (College of Chemistry and Life Science, Guangxi Teachers Education University, Nanning, China)
Guo Nana (College of Chemistry and Life Science, Guangxi Teachers Education University, Nanning, China)
Liu Liangquan (College of Chemistry and Life Science, Guangxi Teachers Education University, Nanning, China)

Various solvent extracts of Phymatopteris hastata, a traditional Chinese medicinal material, were screened for their antioxidant activities. Four systems of in vitro testing were employed to investigate the antiradical and antioxidant effect, i.e., the 2,2-diphenyl-1-picryhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) systems, the hydroxyl radical scavenging assay and the reducing power. In addition, butylated hydroxytoluene (BHT), a widely used synthetic antioxidant, was also studied for comparison. The results revealed that the ethyl acetate extract exhibited outstanding antioxidant activities, which was close or even superior to BHT. Furthermore, the total phenolic (TP) and total flavonoid (TF) contents of different extracts were measured, expressed as gallic acid and rutin equivalent, respectively. The antioxidant activities and the TP/TF content of different extracts followed the same order: ethyl acetate extract > butyl alcohol extract > petroleum ether extract, showing a good correlation between the antioxidant activities and the TP/TF content. The results showed that these extracts, especially the ethyl acetate extract, were rich in phenolics and flavonoids and could be considered as natural antioxidants.

Keywords: total phenolic content, total flavonoid content, DPPH, ABTS, hydroxyl radical, reducing power, Phymatopteris hastata