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Extraction of flavonoids from garden (Salvia officinalis L.) and glutinous (Salvia glutinosa L.) sage by ultrasonic and classical maceration

Veličković Dragan T. (Zdravlje-Actavis Company, Leskovac)
Nikolova Milena T. (Botanički institut, Bugarska Akademija nauka, G. Bončev, Sofija)
Ivancheva Stephanie V. (Botanički institut, Bugarska Akademija nauka, G. Bončev, Sofija)
Stojanović Jelena B. (Zdravlje-Actavis Company, Leskovac)
Veljković Vlada B. (Tehnološki fakultet, Leskovac)

Flavonoids were analyzed in the extracts of garden (Salvia officinalis L.) and glutinous (Salvia glutinosa L.) sage. Ultrasonic extraction (20 minutes at 40°C) and classical maceration (6 h at room temperature) of the extractable substances from dried herbs and dried residual plant materials from which the essential oil had previously been removed by hydrodistillation were performed with petroleum ether, 70 % aqueous solution of ethanol and water. It was found that the extracts from both plants contained flavonoids, but their compositions were dependent of the plant species, the polarity of the extracting solvent and the extraction technique applied. Apigenin and its derivatives (e.g., apigenin 4'-methyl ether), scutellarein 6-methyl ether, isoscutellarein 8-methyl ether, luteolin and 6-OH-luteolin-6-methyl ether where distinctive for S. officinalis. Apigenin, luteolin, 6-OH-luteolin-6-methyl ether, kaempherol 3-methyl ether, kaempherol 3,7-dimethyl ether, quercetin 3,7,3'-trimethyl ether and quercetin 3,7,3',4'-tetramethyl ether were distinctive for S. glutinosa. The flavonoids were also detected in considerable quantities in the plant material from which the essential oils had been already removed. Hence, this industrial waste plant material might be further used as a source of the flavonoids. .

Keywords: garden sage, glutinos sage, Salvia officinalis L., Salvia glutinosa L., flavonoids, ultrasonic extraction, maceration