Archives of Biological Sciences 2012 Volume 64, Issue 3, Pages: 927-934
https://doi.org/10.2298/ABS1203927K
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The antimicrobial activity of honey, bee pollen loads and beeswax from Slovakia
Kacániová Miroslava (Department of Microbiology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Vuković N. (Department of Chemistry, Faculty of Science, Kragujevac)
Chlebo R. (Department of Poultry Science and Small Animals Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Haščík P. (Department of Green’s Biotechnics, Horticulture and Lendscape Engineering Faculty, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Rovná Katarína (Department of Green’s Biotechnics, Horticulture and Lendscape Engineering Faculty, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Cubon J. (Department of Green’s Biotechnics, Horticulture and Lendscape Engineering Faculty, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Džugan Malgorzata (Department of Animal Products Evaluation and Processing, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
Pasternakiewicz Anna (Department of Food Chemistry and Toxicology, University of Rzeszow, Rzeszow, Poland)
The aim of this study was to test the antimicrobial activity of propolis, bee
pollen loads and beeswax samples collected in the year 2009 from two
locations in Slovakia to pathogenic bacteria, microscopic fungi and yeasts.
The antimicrobial effect of the bee product samples were tested using the
agar well diffusion method. For extraction, 99.9% and 70% methanol (aqueous,
v/v) and 96% and 70% ethanol (aqueous, v/v) were used. Five different strains
of bacteria, i.e. Listeria monocytogenes ccM 4699, Pseudomonas aeruginosa ccM
1960; Staphylococcus aureus ccM 3953; Salmonella enterica ccM 4420,
Escherichia coli ccM 3988, three different strains of microscopic fungi,
Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, and seven
different strains of yeasts Candida krusei, Candida albicans, Candida
glabrata, Candida parapsilosis, Candida tropicalis, Geotrichum candidum,
Rhodotorula mucilaginosa, were tested. After 48 hours S. aureus was the
bacterium most sensitive to the 70% ethanol extract of pollen, A. fumigatus
was the most sensitive microscopic fungus (70% ethanol) and C. glabrata the
most sensitive yeast (70% methanol). Microorganisms most sensitive to
propolis extracts were L. monocytogenes, A. fumigatus (70% ethanol) and G.
candidum (70% methanol). Most sensitive to beeswax extracts were E. coli, A.
niger and C. tropicalis.
Keywords: antimicrobial activity, bee products, propolis, bee pollen, beeswax, pathogenic bacteria, microscopic fungi, yeasts