Archives of Biological Sciences 2011 Volume 63, Issue 3, Pages: 907-914
https://doi.org/10.2298/ABS1103907D
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Optimization of cyclosporin A production by Beauveria nivea in continuous fed-batch fermentation
Dong Huijun (Lunan Pharmaceutical Group Corporation, Linyi, PR China)
Jiang Junyun (Lunan Pharmaceutical Group Corporation, Linyi, PR China)
Yan Tongshun (Lunan Pharmaceutical Group Corporation, Linyi, PR China)
Zhao Junjie (Lunan Pharmaceutical Group Corporation, Linyi, PR China)
To develop the effective control method for fed-batch culture of cyclosporin
A production, we chose fructose, L-valine and (NH4)2HPO4 as feeding nutrients
and compared their productivities in relation to different concentrations.
The feeding rate of three kinds of feeding materials was controlled to
maintain the suitable residual concentration. The fed-batch fermentation
results indicated that the optimal concentrations of fructose, L-valine and
(NH4)2HPO4 were about 20 g/L, 0.5 g/L and 0.6 g/L for cyclosporin A
production, respectively. The cultivation of Beauveria nivea could produce
cyclosporin A up to 6.2 g/L for 240 hrs through a continuous
feeding-rate-controlled-batch process under the optimal feeding conditions.
Keywords: cyclosporin A, fed-batch fermentation, Beauveria nivea