Possible activation of murine T lymphocyte through CD98 is independent of interleukin 2/interleukin 2 receptor system

Hiroshi KOMADA; Akiyo IMAI; Emi HATTORI; Morihiro ITO; Hideki TSUMURA; Toshiko ONODA; Mariko KURAMOCHI; Machiko TANI; Kanako YAMAMOTO; Mizuho YAMANE; Mitsuo KAWANO; Machiko NISHIO; Kimitaka YUASA; Myles O'BRIEN; Hidetaka YAMAMOTO; Jun UEMATSU; Masato TSURUDOME; Yasuhiko ITO

doi:10.2220/biomedres.27.61

Full Papers

Possible activation of murine T lymphocyte through CD98 is independent of interleukin 2/interleukin 2 receptor system

Hiroshi KOMADA, Akiyo IMAI, Emi HATTORI, Morihiro ITO, Hideki TSUMURA, Toshiko ONODA, Mariko KURAMOCHI, Machiko TANI, Kanako YAMAMOTO, Mizuho YAMANE, Mitsuo KAWANO, Machiko NISHIO, Kimitaka YUASA, Myles O'BRIEN, Hidetaka YAMAMOTO, Jun UEMATSU, Masato TSURUDOME, Yasuhiko ITO

Author information

Hiroshi KOMADA
Department of Microbiology, Mie University School of Medicine
Department of Microbiology, Suzuka University of Medical Science
Akiyo IMAI
Department of Microbiology, Suzuka University of Medical Science
Emi HATTORI
Department of Microbiology, Suzuka University of Medical Science
Morihiro ITO
Department of Microbiology, Mie University School of Medicine
Hideki TSUMURA
Division of Laboratory Animal Resources, National Research Institute for Child Health and Development
Toshiko ONODA
Department of Microbiology, Suzuka University of Medical Science
Mariko KURAMOCHI
Department of Microbiology, Suzuka University of Medical Science
Machiko TANI
Department of Microbiology, Suzuka University of Medical Science
Kanako YAMAMOTO
Department of Microbiology, Suzuka University of Medical Science
Mizuho YAMANE
Department of Microbiology, Suzuka University of Medical Science
Mitsuo KAWANO
Department of Microbiology, Mie University School of Medicine
Machiko NISHIO
Department of Microbiology, Mie University School of Medicine
Kimitaka YUASA
Department of Microbiology, Mie University School of Medicine
Myles O'BRIEN
Mie Prefectural College of Nursing
Hidetaka YAMAMOTO
Department of Microbiology, Suzuka University of Medical Science
Jun UEMATSU
Department of Microbiology, Suzuka University of Medical Science
Masato TSURUDOME
Department of Microbiology, Mie University School of Medicine
Yasuhiko ITO
Department of Microbiology, Mie University School of Medicine

JOURNAL FREE ACCESS

2006 Volume 27 Issue 2 Pages 61-67

DOI https://doi.org/10.2220/biomedres.27.61

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Abstract

CD98 is a widely expressed cell surface heterodimetric protein of 125 kDa. Its expression is upregulated during lymphocyte activation induced by mitogen, superantigen, conventional antigen, and a combination of phorbol myristate acetate (PMA) and ionomycin. However, the role of CD98 in the immune system is not so well understood. The role of CD98 in murine T lymphocyte proliferation was investigated, especially in correlation with the interleukin 2 (IL-2)/interleukin 2 receptor (IL-2R) system. Monoclonal antibody (mAb) directed against murine CD98 heavy chain (mCD98HC) suppressed the proliferation of lymphocytes stimulated with concanavalin A (Con A). Anti-mCD98HC mAb did not suppress the expression of IL-2Rα. Anti-IL-2Rα mAb, which suppressed DNA synthesis, did not inhibit the expression of CD98HC. Murine IL-2 (recombinant), which induced considerable DNA synthesis by lymphocytes stimulated with a sub-optimal dose of Con A, did not induce CD98HC expression in lymphocytes. In addition, anti-mCD98HC mAb did not inhibit the production of IL-2 by lymphocyte stimulated with Con A. Taken together with these findings, it was speculated that the CD98 system is independent of the IL-2/IL-2R system in murine T lymphocyte activation.

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