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Development and performance of a multiplex PCR assay for the detection of bacteria in sterile body fluids

    Coreen Johnson

    *Author for correspondence:

    E-mail Address: cljohnso@texaschildrens.org

    Department of Pathology, Texas Children’s Hospital, Houston, TX 77030, USA

    Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030, USA

    ,
    Christopher Marquez

    Department of Laboratory Medicine & Pathology, Mayo Clinic, Jacksonville, FL 32224, USA

    ,
    Damon Olson

    Department of Pathology, Children’s Minnesota, Minneapolis, MN 55404, USA

    ,
    Tabitha Ward

    Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030, USA

    ,
    Stephen Cheney

    Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030, USA

    ,
    Tina Hulten

    Department of Pediatrics, Section of Infectious Disease, Baylor College of Medicine, TX 77030, USA

    ,
    Trang Ton

    Department of Pathology, Texas Children’s Hospital, Houston, TX 77030, USA

    ,
    CR Webb

    Department of Pathology, Texas Children’s Hospital, Houston, TX 77030, USA

    &
    James Dunn

    Department of Pathology, Texas Children’s Hospital, Houston, TX 77030, USA

    Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030, USA

    Published Online:https://doi.org/10.2217/fmb-2022-0226

    Aim: To assess the performance characteristics of a lab-developed multiplex PCR assay for the detection of common bacterial pathogens associated with infections in pediatric patients from normally sterile sites, such as cerebrospinal fluid, synovial and pleural fluids. Materials & methods: A total of 272 specimens were tested by PCR and traditional culture methods to assess the presence of Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus pyogenes, methicillin-sensitive and methicillin-resistant Staphylococcus aureus and Kingella kingae. Results: Compared with culture, the overall positive and negative percentage agreement of the PCR were 95.9% and 74.1%, respectively. Conclusion: This sterile body fluid PCR affords a rapid and sensitive alternative for bacterial detection, allowing for more timely pathogen-directed antimicrobial therapy.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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