It is important to estimate environmentalloads by road traffic in composition of urban transportation planning as well as land use planning. Because there is close relationship between traffic and land use in urban area. This study aims to propose an estimation system which can estimate environmental loads by road traffic. The system is based on traffic analysis taking account of urban land use using GIS. In this study, environmental loads by road traffic are “air pollution”, “vibration” and “noise”. The system is applied to Tokushima urban area, and estimated values are compared to measured data in order to inspect the accuracy of the system. As a result of evaluation for this system, it has adequate level for estimation of the environmental loads by interaction road traffic andurban landuse.