تأثیر روش ساخت بر ریزساختار و خواص مکانیکی داربست های کامپوزیتی کلسیم فسفات/ ژلاتین- نشاسته

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مهندسی مواد و متالورژی، دانشکده مهندسی، دانشگاه فردوسی مشهد، مشهد، ایران

2 استادیار/ دانشکده مهندسی دانشگاه فردوسی مشهد گروه مهندسی متالورژی و مواد

3 استادیار، گروه مهندسی مواد و متالورژی، دانشکده مهندسی، دانشگاه فردوسی مشهد، مشهد، ایران

4 گروه فارماکولوژی و سم شناسی، دانشکده داروسازی، دانشگاه علوم پزشکی مشهد، ایران

چکیده

وقوع آسیب های استخوانی مانند شکستگی های بزرگ استخوان در اثر عوامل متعدّد غیرقابل انکار است. در این موارد به داربستی نیاز است تا به کمک آن، منطقه آسیب دیده ترمیم شود. هدف از این پژوهش، ساخت و بررسی خواص مکانیکی داربست های کلسیم فسفات/ ژلاتین- نشاسته است. بدین منظور پس از سنتز ذرّات کلسیم فسفات به روش سل ژل، از سه روش مختلف برای ساخت داربست کامپوزیتی کلسیم فسفات-ژلاتین استفاده شد. درنهایت روشی که دارای ریزساختار کاملاً متخلخل و مطلوب بود، به عنوان روش بهینه جهت ساخت داربست های کلسیم فسفات/ ژلاتین- نشاسته انتخاب گردید. در ساخت کامپوزیت ها از 4 درصد وزنی مختلف نشاسته در محلول ژلاتین استفاده و تأثیر پوشش ژلاتینی دارای اتّصالات عرضی با گلوتارآلدهید نیز بررسی گردید. طبق نتایج حاصل از آزمون مکانیکی، نمونه حاوی 60درصد ژلاتین و 40درصد نشاسته، دارای استحکام خمشی بهینه بوده که مقدار آن، 2.3 مگاپاسکال برای نمونه بدون پوشش و 4.5 مگاپاسکال مربوط به نمونه دارای پوشش است. همچنین نتایج حاصل از تصویربرداری با میکروسکوپ الکترونی روبشی(SEM)، وجود شبکه کاملاً متخلخل با حفرات به هم پیوسته را در سطح نمونه های پوشش داده شده و حضور تخلخل های موضعی در ساختار متراکم را در قسمت های داخلی آن نشان می دهد.نتایج کشت سلولهای بنیادی پالپ دندان عقل نیز زیست سازگاری و وجود قابلیت چسبندگی و تکثیر سلولهای بنیادی برروی داربست را اثبات میکند.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Manufacturing Route on Microstructure and Mechanical Properties of Calcium Phosphate/Gelatin-Starch Composite Scaffold

نویسندگان [English]

  • Faezeh Darvishian Haghighi 1
  • Sahar Mollazadeh Beidokhti 2
  • Samaneh Sahebian Saghi 3
  • Zahra Tayarani Najaran 4
1 Department of Materials and Metallurgy engineering, Faculty of engineering, Ferdowsi University of Mashhad, Mashhad, Iran
2 Assistant Professor, Department of Materials and Metallurgy engineering, Faculty of Engineering, Ferdowsi University of Mashhad, Mashhad, Iran
3 Assistant Professor, Department of Materials and Metallurgy engineering, Faculty of Engineering, Ferdowsi University of Mashhad, Mashhad, Iran
4 Department of Pharmacodynamics and Toxicology, Mashhad University of Medical Sciences, Iran
چکیده [English]

There are plenty of bone damages due to different reasons these days. In order to heal of damaged area, utilizing of the scaffold is necessary. The main aim of this research is fabrication and investigation of Calcium Phosphate/Gelatin-Starch composite scaffolds. After synthesizing the Calcium Phosphate particles via sol gel route, three different methods were used to manufacture Calcium Phosphate/ Gelatin scaffolds. The method which has the porous structure was selected as the main method for fabricating Calcium Phosphate/ Gelatin-Starch scaffolds. Weight percentage ofstarch and applying the Gelatin-Glutaraldehyde coating were chosen as two variations. According to mechanical properties results, the sample with 60 weight percentage of Gelatin and 40 weight percentage of Starch has the highest final flexural strength which are 4.5 and 2.3 MPa for samples with and without Gelatin-Glutaraldehyde coating respectively. The SEM results show the completely porous structure with interconnected pores on the surface of coated samples and some local pores in internal parts of the scaffolds.According to the result of cell culture, these scaffolds provide surfaces that facilitate the response of stem cells related to attachment, survival, and proliferation.

کلیدواژه‌ها [English]

  • Scaffold
  • Gelatin
  • Starch
  • Calcium Phosphate Particles
  • flexural Strength

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  • تاریخ دریافت: 27 بهمن 1397
  • تاریخ بازنگری: 25 آبان 1398
  • تاریخ پذیرش: 03 آذر 1398

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