Assessment of Organophosphate Pesticides Exposure in Men with Idiopathic Abnormal Semen Analysis: A Cross-Sectional Pilot Study

Document Type : Original Article

Authors

1 Department of Biochemis try, Jawaharlal Ins titute of Pos tgraduate Medical Education and Research, Pondicherry, India

2 Department of Obs tetrics and Gynecology, Jawaharlal Ins titute of Pos tgraduate Medical Education and Research, Pondicherry, India

3 Department of Pathology, Jawaharlal Ins titute of Pos tgraduate Medical Education and Research, Pondicherry, India

Abstract

Background:
Because of the widespread use of organophosphate (OP) pesticides in agriculture, they are major environmental contaminants in developing countries. OP pesticides decrease sperm concentration and affect its quality, viability, and motility. Studies have demonstrated the association between abnormal semen analysis and OP pesticides exposure among the high-risk population. Asthere is limited data on the percentage of OP pesticides exposure, the study aimed to determine the OP pesticides exposure in Southern Indian men with idiopathic abnormal semen analysis and find the possible source of their OP pesticides exposure.
 
Materials and Methods:
 In this cross-sectional pilot study, fifty men with idiopathic abnormal semen analysis as cases and fifty men with normal semen analysis as controls were recruited. Detailed history wastaken and general and systemic examinations were carried out. OP pesticides exposure was determined by assessment of pseudocholinesterase and acetylcholinesterase levels and urinary OP pesticides metabolites dialkyl phosphate (DAP) consisting of dimethyl phosphate (DMP), diethyl thiophosphate (DETP), and diethyl dithiophosphate (DEDTP).
 
Results:
 Cases had statistically significantly lower levels of pseudocholinesterase (5792.07 ± 1969.89 vs. 10267.01 ± 3258.58 IU/L) (P=0.006) and acetylcholinesterase [102.90 (45.88-262.74) vs. 570.31 (200.24-975.30) IU/L] (P=0.001) as compared to controls. Cases had a statistically significantly higher percentage of urinary DAP positivity as compared to controls (80 vs. 38%) (p <0.0001). Hence, cases had a significantly higher percentage of OP pesticides exposure as compared to controls (20 vs. 4 %) (P=0.015). OP-exposed cases had significantly higher urinary DETP and DEDTP levels as compared to OP non-exposed cases. Also, urinary DETP and DEDTP levels were significantly negatively associated with sperm concentration, motility, and normal morphology among OP-exposed cases.
Conclusion: Southern Indian men with idiopathic abnormal semen analysis had a significantly higher percentage of OP pesticides exposure as compared to men with a normal semen analysis.

Keywords

References

1. Zegers-Hochschild F, Adamson GD, de Mouzon J, Ishihara O, Mansour R, Nygren K, et al. International Committee for Monitoring Assis ted Reproductive Technology (ICMART) and the World Health Organization (WHO) revised glossary of ART terminology, 2009. Fertil S teril. 2009; 92: 1520-1524.
2. Elhussein OG, Ahmed MA, Suliman SO, Yahya LI, Adam I. Epidemiology of infertility and characteris tics of infertile couples reques ting assis ted reproduction in a low-source setting in Africa, Sudan. Fertil Res Pract. 2019; 5: 7.
3. Adamson PC, Krupp K, Freeman AH, Klausner JD, Reingold AL, Madhivanan P. Prevalence & correlates of primary infertility among young women in Mysore, India. Indian J Med Res. 2011; 134(4): 440-446.
4. Kumar N, Singh AK. Trends of male factor infertility, an important cause of infertility: A review of literature. J Hum Reprod Sci. 2015; 8(4): 191-196.
5. Levine H, Jørgensen N, Martino-Andrade A, Mendiola J, Weksler- Derri D, Milndlis I, et al. Temporal trends in sperm count: a sys temic review and meta-regression analysis. Hum Reprod Update. 2017: 23(6): 646-659.
6. Sengupta P, Dutta S, Krajewska-Kulak E. The disappearing sperms: analysis of reports published between 1980 and 2015. Am J Mens Health. 2017; 11(4): 1279-1304.
7. Mishra P, Negi MPS, Srivas tava M, Singh K, Rajender S. Decline in seminal quality in Indian men over the las t 37 years. Reprod BiolEndocrinol. 2018; 16(1): 103.
8. Bonovoisin T, Utyasheva L, Knipe D, Gunnell D, Eddles ton M. Suicide by pes ticide poisoning in India: a review of pes ticide regulation and their impact on suicide trends. BMC Public Health. 2020; 20(1): 251.
9. Kumar S, Kaushik G, Villarreal-Chiu JF. Scenario of organophosphate pollution and toxicity in India: a review. Environ Sci Pollut Res Int. 2016; 23(10): 9480-9491.
10. Perry MJ, Venners SA, Chen X, Liu X, Tang G, Xing H, et al. Organophosphorous pes ticide exposures and sperm quality. Reprod Toxicol. 2011; 31(1): 75-79.
11. Rodriguez H, Bus tos-Obregon E. An in vitro model to evaluate the effect of an organophosphoric agropes ticide on cell proliferation in mouse seminiferous tubules. Andrologia. 2000; 32(1): 1-5.
12. Contreras HR, Bus tos-Obregon E. Morphological alterations in mouse tes tis by a single dose of malathion. J Exp Zool. 1999; 284(3): 355-359.
13. Ghafouri-Khosrowshahi A, Ranjbar A, Mousavi L, Nili-Ahmadabadi H, Ghaffari F, Zeinvand-Lores tani H, et al. Chronic exposure to organophosphate pes ticides as an important challenge in promoting reproductive health: a comparative s tudy. J Educ Health Promot. 2019; 8: 149.
14. Miranda-Contreras L, Cruz I, Osuna JA, Gómez-Pérez R, Berrueta L, Salmen S, et al. Effects of occupational exposure to pes ticides on semen quality of workers in an agricultural community of Merida s tate, Venezuela. Inves t Clin. 2015; 56(2): 123-136.
15. Cremonese C, Piccoli C, Pasqualotto F, Clapauch R, Koifman RJ, Koifman S, et al. Occupational exposure to pes ticides, reproductive hormones levels and sperm quality in young Brazilian men. Reprod Toxicol. 2017; 67: 174-185.
16. Yucra S, Gasco M, Rubio J, Gonzales GF. Semen quality in Peruvian pes ticide applicators: association between urinary organophosphate metabolites and semen parameters. Environ Health. 2008; 7: 59.
17. Jamal F, Haque QS, Singh S, Ras togi SK. The influence of organophosphate and carbamate on sperm chromatin and reproductive hormones among pes ticide sprayers. Toxicol Ind Health. 2016; 32(8): 1527-1536.
18. Daoud S, Sellami A, Bouassida M, Kebaili S, Ammar Keskes L, Rebei T, et al. Routine assessment of occupational exposure and its relation to semen quality in infertile men: a cross-sectional s tudy. Turk J Med Sci. 2017; 47(3): 902-907.
19. Lotti F, Corona G, Vitale P, Maseroli E, Fino MG, Maggi M. Current smoking is associated with lower seminal vesicles and ejaculate volume, despite higher tes tos terone levels, in male subjects of infertile couples. Hum Reprod. 2015; 30(3): 590-602.
20. Matsumoto S, Beeson WL, Shavlik DJ, Siapco G, Jaceido-Sieg l, Fraser G, et al. Association between vegetarian diet and cardiovascular risk factors in non-Hispanic white participants of the Adventis t Health S tudy-2. J Nutr Sci. 2019; 8: e6.
21. Dalvi TM, Khairnar MR, Kalghatgi SR. An Update of B.G. Prasad and Kuppuswamy socioeconomic s tatus classification scale for Indian population. Indian J Pediatr. 2020; 87(7): 567-568.
22. World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. Cambridge: Cambridge University Press; 1999.
23. Ellman GL, Courtney KD, Andres V Jr, Feather-S tone RM. A new and rapid colorimetric determination of acetylcholines terase activity. Biochem Pharmacol. 1961; 7: 88-95.
24. Hemakanthi De Alwis GK, Needham LL, Barr DB. Determination of dialkyl phosphate metabolites of organophosphorous pes ticides in human urine by automated solid-phase extraction, derivatization and gas chromatography mass spectrometry. J Anal Toxicol. 2008; 32(9): 721-727.
25. Hornung RW, Reed LD. Es timation of average concentration in the presence of nondetectable values. Appl Occup Environ Hyg. 1990; 5(1): 46-51.
26. Nicolopoulou-S tamati P, Maipas S, Kotampasi C, S tamatis P, Hens L. Chemical pes ticides and human health: The urgent need for a new concept in agriculture. Front Public Health. 2016; 4: 148.
27. Nishant N, Upadhyay R. Presence of pes ticide residue in vegetable crops: a review. Agricultural Review. 2016; 37(3): 173-185.
28. Mehrpour O, Karrari P, Zamani N, Tsatsakis AM, Abdollahi M. Occupational exposure to pes ticides and consequences on male semen and fertility: a review. Toxicol Lett. 2014; 230(2): 146-156.
29. Kaur K, Kaur R. Occupational pes ticide exposure, impaired DNA repair, and diseases. Indian J Occup Environ Med. 2018; 22(2): 74-81.
30. Rehman S, Usman Z, Rehman S, AlDraihem M, Rehman N,Rehman I, et al. Endocrine disrupting chemicals and impact on male reproductive health. Transl Androl Urol. 2018; 7(3): 490-503.
31. Gracia-Lor E, Cas tiglioni S, Bade R, Cas trignanò E, González-Mariño I, Hapeshi E, et al. Measuring biomarkers in was tewater as a new source of epidemiological information: current s tate and future perspectives. Environ Int. 2017; 99: 131-150.
32. Glorennec P, Serrano T, Fravallo M, Warembourg C, Monfort C, Cordier S, et al. Determinants of children’s exposure to pyrethroid insecticides in wes tern France. Environ Int. 2017; 104: 76-82.
33. Katole A, Saoji AV. Prevalence of primary infertility and its associated risk factors in urban population of central India: a community-based cross-sectional s tudy. Indian J Community Med. 2019; 44(4): 337-341.
34. Dutta S, Bahadur M. Effect of pes ticide exposure on the cholines terase activity of the occupationally exposed tea garden workers of northern part of Wes t Bengal, India. Biomarkers. 2019; 24(4): 317-324.
35. Li AJ, Kannan K. Urinary concentrations and profiles of organophosphate and pyrethroid pes ticide metabolites and phenoxyacid herbicides in populations in eight countries. Environ Int. 2018; 121(Pt 2): 1148-1154.
36. Muñoz-Quezada MT, Iglesias V, Lucero B, S teenland K, Barr DB, Levy K, et al. Predictors of exposure to organophosphate pes ticides in schoolchildren in the Province of Talca, Chile. Environ Int. 2012; 47: 28-36.
International Journal of Fertility and Sterility
Volume 15, Issue 3
July 2021
Pages 219-225

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