مقایسه کاربرد برگی و خاکی نانوکلات‌های آهن بر ویژگی‌های رشدی آفتابگردان در یک خاک شور

نوع مقاله : مقاله کامل علمی پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی‌ارشد ، گروه علوم خاک، دانشگاه زابل

2 دانشیار ، گروه علوم خاک، دانشگاه زابل،

3 دانشیار ، گروه شیمی، دانشگاه زابل

چکیده

سابقه و هدف: فناوری‌های نوین مانند بیوتکنولوژی و نانوتکنولوژی نقش مهمی در افزایش تولیدات و بهبود کیفیت مواد‌ غذایی تولید شده توسط کشاورزان ایفا می‌کنند. شوری خاک نیز مستقیماً بر جذب عناصر غذایی اثرگذار می‌باشد. هدف از انجام این تحقیق، بررسی کاربرد نانو کلات با بنیان‌های اتیلن‌دی‌آمین‌تترا استیک‌اسید (EDTA) و اسید هیومیک (HA) به عنوان عامل کودی می‌باشد.
مواد و روش‌ها: این تحقیق به صورت آزمایش گلدانی و به صورت فاکتوریل در قالب طرح کاملاً تصادفی شامل دو سطح کودی نانو کلات‌های آهن (اتیلن‌دی‌آمین‌تترا استیک‌اسید و اسید هیومیک) و دو روش کاربرد کود (محلول‌پاشی با سطح یک گرم در لیتر و خاکی با سطح 1 گرم در کیلوگرم همراه با شاهد) در سه تکرار در گلخانه تحقیقاتی دانشگاه زابل انجام شد. جهت کشت از 5 بذر آفتابگردان استفاده گردید. همچنین اعمال تیمارها به دو صورت مصرف خاکی و محلول‌پاشی از زمان 6-4 برگی، در 4 مرحله و در فواصل زمانی 15 روزه اجرا گردید. در طول دوره آزمایش نیز گلدان‏ها توسط آب مقطر و به روش وزنی آبیاری شدند. 8 هفته بعد از کشت گیاه، برخی پارامترهای رشد، میزان کلروفیل و همچنین عناصر غذایی (آهن، روی، فسفر و پتاسیم) اندام هوایی گیاه آفتابگردان (Helianthus annuus) در شرایط شوری دایمی خاک بررسی گردید. تجزیه آماری بر اساس نرم افزار آماری SAS و آنالیز واریانس داده‏ها بر اساس برنامه تجزیه واریانس آنووا انجام شد. میانگین شاخص‏های اندازه‌گیری شده با استفاده از آزمون دانکن در سطح 05/0 گروه‏بندی شدند.
یافته‌ها: نتایج بدست آمده نشان داد اثرات متقابل نوع کود و روش مصرف آن تأثیر معنی‌داری بر شاخص‌های اندام‌هوایی (وزن خشک، تعداد برگ و ارتفاع بوته)، محتوای کلروفیل‌های برگ (a، b، کل و کارتنوئیدها) و غلظت عناصر غذایی (آهن، روی، فسفر و پتاسیم) گیاه آفتابگردان داشت (p≤0.01). مقایسه میانگین‌ها نشان داد محلول‌پاشی نانو کلات آهن با بنیان اتیلن‌دی‌آمین‌تترا استیک‌اسید وزن خشک اندام هوایی، تعداد برگ و ارتفاع بوته (به ترتیب 15/1، 21/1 و 05/1 برابر)، میزان کارتنوئید برگ (76/1 برابر) و همچنین غلظت آهن در اندام هوایی (37/2 برابر) را در مقایسه با شاهد افزایش داد. همچنین در مصرف خاکی نانو کلات آهن با بنیان اتیلن-دی‌آمین‌تترا استیک‌اسید، میزان غلظت عناصر فسفر و پتاسیم نیز به ترتیب 59/1 و 94/1 برابر نسبت به شاهد افزایش یافت.
نتیجه‌گیری: با توجه به نتایج، کاربرد محلول‌پاشی نانوکلات آهن با بنیان اتیلن‌دی‌آمین‌تترا استیک‌اسید اکثر پارامترهای رشد گیاه آفتابگردان را بهبود داد که برتری این نانو کود را نسبت به نانوکلات آهن با بنیان اسید هیومیک در خاک‌های شور نشان می‌دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Comparison of Foliar and Soil Application of Iron Nano-Chelate on Sunflower Growth Characteristics in a Saline Soil

نویسندگان [English]

  • Marzyeh Shahraki Zad 1
  • Ahmad Gholamalizadeh 2
  • Noshin Mir 3
1 Soil Science Departement Faculty of Soil and Water University of Zabol
2
3 Departement of Chemistry Faculty of Sciences University of Zabol
چکیده [English]

Abstract
Background and objectives: Modern technologies, such as biotechnology and nanotechnology, play an important role in increasing production and improving the quality of food produced by farmers. Salinity also directly affects the absorption of nutrients elements. This study aims to investigate the use of nano-chelate with ethylene diamine tetra-acetic acid (EDTA) and Humic Acid (HA) base as fertilizer agents on Sunflower.
Materials and methods: This study was done as a pot experiment as a factorial in a completely randomized design including two levels of iron nano-chelate fertilizer (ethylene diamine tetra-acetic acid and Humic Acid) and two methods of application of fertilizers (foliar application with the level of one gram per Liter and soil application with the level of one gram per kilogram with the control) in three replications in the research greenhouse of Zabol University. Five sunflower seeds were used for cultivation. The treatments were applied in two ways that are soil use and spraying form, when plants are 4 to 6 leaves the tratments have been used in 4 steps and at intervals of 15 days. The pots were irrigated by distilled water and weighting method during the experiment period. Some growth parameters, chlorophyll content as well as nutrients (Fe, Zn, P, K) of sunflower shoot (Helianthus annuus) under permanent salinity conditions were investigated about two months after planting. Statistical analysis was performed on the basis of SAS software and Analysis of data variance was done based on ANOVA software. The mean of measured indices was categorized using Duncan test at the level of 0.05.
Results: Results showed that the interactions of fertilizer type and its method of use have a significant effect on the shoot indexes (dry weight, leaf number and plant height), leaf chlorophyll content (a, b, total and carotenoids) and nutrient concentration (Fe, Zn, P, K) of sunflower plant (p≤0.01). Comparison of the means showed that spraying EDTA-based iron nano-chelate increases dry weight of shoot, the number of leaves and plant height (1.15, 1.21 and 1.05 times, respectively), the level of leaf carotenoid (1.76 times) and also Fe concentration in the shoot (2.37 times) compared to the control. Moreover, in EDTA-based iron nano-chelate soil application, the concentration of phosphorus and potassium elements is respectively increased 1.59 and 1.94 than control.
Conclusion: According to the results, spraying EDTA-based iron nano-chelate improved most of the sunflower growth parameters, which shows the superiority of this Nano-fertilizer to HA-based iron Nano-chelate in saline soils.

کلیدواژه‌ها [English]

  • Chelate
  • Micronutrients
  • Nano-Fertilizer
  • Nanotechnology
  • Oil Plants
 1.Abdolmaleki, A.S., Ahangar, A.G.,and Soltani, J. 2013. Artificial Neural Network (ANN) approach for predicting Cu concentration in drinking water of Chahnimeh1 Reservoir in Sistan-Balochistan, Iran. Health Scope.2: 1. 31-8.
2.Alidoust, D., and Isoda, A. 2013. Effect of gamma Fe2O3 nanoparticles on photosynthetic characteristic of soybean (Glycine max (L.) Merr.): foliar spray versus soil amendment. Acta Physiologiae Plantarum. 35: 12. 3365-75.
3.Arnon, D.I. 1949. Copper enzymes in isolated chloroplast, polyphenol oxidase in Beta vulgaris. Journal of Plant Physiology. 24: 1-75.
4.Askary, M., Amirjani, M.R., and Saberi, T. 2017a. Comparision of the effects of nano-iron fertilizer with iron-chelate on growth parameters and some biochemical properties of Catharanthus roseus. Journal of Plant Nutrition. 40: 7. 974-982.
5.Askary, M., Talebi, S.M., Amini, F.,and Balout Bangan, A.D. 2017b.Efects of iron nanoparticles on Mentha piperita L. under salinity stress. Biologija. 63: 1. 65-75.
6.Azizpour, K., Shakiba, M.R., Khosh Kholgh Sima, N., Alyari, H., Moghaddam, M., Esfandiari, E., and Pessarakli, M. 2010. Physiological response of spring durum wheat genotypes to salinity. Journal of Plant Nutrition. 33: 859-873.
7.Bouyoucos, C.J. 1997. Hydrometer method improved for making particle size analysis of soil. Agronomy Journal. 54: 464-465.
8.Bower, C.A., Reitemeier, R.F., and Fireman, M. 1952. Exchangeable cation analysis of saline and alkali soils. Soil Science. 73: 251-261.
9.Chapman, H.D., and Pratt, P.F. 1962. Methods of Analysis for Soil, Plant and Water. Soil Science. 93: 68-72.
10.Desingh, R., and Kanagaraj, G.2007. Influence of salinity stress on photosynthesis and antioxidative systems in two cotton varieties.General and Applied Plant Physiology. 33: 221-234.
11.Di Caterina, R., Giuliani, M.M., Rotunno, T., De Caro, A., and Flagella, Z. 2007. Influence of salt stress on seed yield and oil quality of two sunflower hybrids. Annals and Applied Biology.  151: 145-154.
12.El-Ashry, M.T., Shilfaarde, J.V., and Schiffman, S. 1985. Salinity Pollution from irrigated agriculture. Journal of Soil Water Conservation. 40: 48-52.
13.Fiorentino, G., Spaccini, R., and Piccolo, A. 2006. Separation of molecular constituents from a humic acid by solid-phase extraction following a transesterification reaction. Talanta. 68: 1135-1142.
14.Godsey, R.J., and Johnson, B. 2001. Seed treatment, seeding rate, and cultivar effects on iron deficiency chlorosis of soybean. Journal of Plant Nutrition. 24: 1255-1268.
15.Golshahi, S., Ahangar, A.Gh., Mir, N., and Ghorbani, M. 2017. The effect of foliar application of iron sources on growth parameters, iron concentration and activity of some enzymes of sorghum. Journal of Water and Soil.31: 5. 1467-1480. (In Persian)
16.Golshahi, S., Ahangar, A.Gh., Mir, N., and Ghorbani, M. 2018. A comparison of the use of different sources of nanoscale iron particles on the concentration of micronutrients and plasma membrane stability in sorghum. Journal of Soil Science and Plant Nutrition. 18: 1. 236-252.
17.Helmke, P.A., and Sparks, D.L. 1996. Lithium, sodium, potassium, cesium, and rubidium. P. 551-574. In: D.L. Sparks, (ed.), Methods of soil analysis. Part 3. Chemical methods and processes. Madison: Soil Science Society of America, Madison, WI.
18.Hu, J., Guo, H., Li, J., Gan, Q., Wang, Y., and Xing, B. 2017. Comparative impacts of iron oxide nanoparticles and ferric ions on the growth of Citrus maxima. Environmental Pollution. 221: 199-208.
19.Hue, N.V., Craddock, G.R., and Adams, F. 1986. Effect of organic acids on aluminium toxicity in subsoils. Soil Science Society of American Journal. 50: 28-34.
20.Hwang, Y.S., Liu, J., Lenhart, J.J., and Hadad, C.M. 2007. Surface complexes of phthalic acid at the hematite/water interface. Journal of Collide and Interface Science. 307: 124-134.
21.Ills, E., and Tombacz, E. 2003. The role of variable surface charge and surface complexation in the absorption of
humic acid on magnetite. Colloids and Surfaces. 230: 99-109.
22.Jakson, M.L. 1967. Soil Chemical Analysis. Prentice-Hall of India Private Limited, New Dehli, 498p.
23.Javan Siah Bigdello, S., Pirzad, A., and Hasani, A. 2012. The effects of foliar application of iron nanoparticles in  comparison with the conventional three-iron fertilizer on morphological performance of marigold. p. 1. 2nd National Conference on biological diversity and its impact on agriculture and the environment, 11 Jul. 2012. Agriculture and . (In Persian)
24.Jokar, L., and Ronaghi, A. 2015. Effect of foliar application of different Fe levels and sources on growth and concentration of some nutrients in sorghum. Journal of Science and Technology of Greenhouse Culture.6: 2. 163-174. (In Persian)  
25.Katerij, N., Van Hoorn, J.W., Hamdy, A., Karam, F., and Mastrorilli, M. 1996. Effect of salinity on emergence and on water stress and early seedling growth of sunflower and maize. Agricultural Water Management. 26: 81-91.
26.Lee, C.W., Mahendra. S., Zodrow, K., Li, D., Tsai, Y.C., and Braam, J., et al., 2010. Developmental phytotoxicity of metal oxide nanoparticles to Arabidopsis thaliana. Environmental Toxicology and Chemistry. 29: 669-75.
27.Lindsay, W.L., and Norvell, W.A. 1978. Development of DTPA Soil test for Zinc, Iron, Manganese and Copper. Soil Science Society of American Journal. 42: 421-428.
28.Liu, J.F., Zhao, Z.S., and Jiang,G.B. 2008. Coating Fe3O4 magnetic nanoparticles with humic acid for high efficient removal of heavy metals in water. Environmental Science and Technology. 42: 6949-6954.
29.Martínez-Fernández, D., Barroso, D., and Komárek, M. 2016. Root water transport of Helianthus annuus L. under iron oxide nanoparticle exposure. Environmental Science and Pollution Research. 23: 1732-1741.
30.Masonic, A., Evacoli, A., and Mavoti, M. 1996. Spectral of leaves deficient in iron sulphur, magnesium and magnese. Agronomy Journal. 88: 6. 937-943.
31.Matlabi Talaee, A., Tajbakhsh, M., Hasanzadeh Ghort Tappe, A., and Roshdi, M. 2011. The effect of time and different concentrations of sprayed with nano-oxide fertilizers and chelate iron on quantitative and qualitative characteristics of sorghum in the second crop. National Conference on Advances in agriculture, 15-16 Nov. 2011. Islamic Azad University of Shahre Ghods, Shahre Ghods, Tehran. (In Persian)
32.Mosuavi, S.R., and Rezaei, M. 2011. Nanotechnology in agriculture and food production. Journal of Applied Environmental and Biological Science. 1: 414-419.
33.Naderi, M., and Danesh Shahraki,A. 2012. The role of nanotechnology in improves the efficiency of chemical fertilizer nutrient. Journal of Nanotechnology. 22:4-5.
34.Namduri, H., and Nasrazadani, S. 2008. Quantitative analyses of iron oxides using Fourier transform infrared spectrophotometry. Corrosion Science. 50: 2493-2497.
35.Olsen, S.R., Cole, C.V., Watanabe, F.S., and Dean, L.A. 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. USDA Circular 939, US Gov. Printing Office, Washington, DC, 19p.
36.Patton, C., and Crouch, S. 1977. Determination of serum urea enzymatically. Analytical Chemistry. 49: 464-469.
37.Perkin, E. 1982. Analytical Methods for Atomic Absorption Spectrophotometry. Perkin Elmer Corporation. Connecticut, USA, 310p.
38.Piccolo, A. 2002. The supramolecular structure of humic substances. A novel understanding of humus chemistry and implications in soil science. Advances in Agronomy. 75: 57-134.
39.Rahimian Boger, A., Salehi, H., and Jahanandish, S. 2011. The effect of Nanofertilizer iron chelated on quantitative and qualitative characteristics of cut flower varieties ''fysta'', ''shyraz'' and ''sourbyt Avalanch'' in a hydroponic system. 1nd National Conference
on Modern Agricultural Sciences& Technologies, 10-12 Sep. 2011. University of Zanjan, Zanjan. Available at http://www.civilica.com/ Paper-MAST01-MAST01_952.html. (In Persian)  
40.Ravi, S., Channel, H.T., Hebsur, N.S., Patil, B.N., and Dharmtti, P.R. 2008. Efect of sulphur, zinc and iron nutrition on growth, yield, nutrient uptake and quality of safower (Carthamus tinctorius L.). Karnataka Journal of Agricultural  Scicence. 21: 382-5.
41.Redondo-Gomez, S., Andres-Moreno, L., Mateos-Naranjo, E., Parra, P., Valera-Burgos, J., and Aroca, R. 2011. Synergic effect of salinity and zinc stress on growth and photosynthetic responses of the cordgrass Spartina densiflora. Journal of Experimental Botany. 62: 5521-30.
42.Rhoades, J.D. 1982. Soluble salts.P. 167-179. In: A.L. Page (ed.), Methods of soil analysis. Part 2: Chemical and microbiological properties. Monograph Number 9 (Second Edition). ASA, Madison, WI.
43.Rizwan, M., Ali, S., Qayyum, M.F., Ok, Y.S., Adrees, M., Ibrahim, M., Zia-ur-Rehman, M., Farid, M., and Abbas, F. 2017. Effect of metal and metal oxide nanoparticles on growth and physiology of globally important food crops: A critical review. Journal of Hazardous Materials. 322: 2-16.
44.Rui, M., Ma, C., Hao, Y., Guo, J., Rui, Y., Tang, X, Zhao, Q., Fan, X., Zhang, Z., Hou, T., and Zhu, S. 2016. Iron oxide nanoparticles as a potential iron fertilizer for peanut (Arachis hypogaea). Frontiers in Plant Science. 7: 815.
Doi: 10.3389/fpls.2016.00815.
45.Shailesh, K.D., Pramod, M., Rajashri, K., and Anand, K. 2013. Effect of nanoparticles suspension on the growth of Mung (Vigna radiata) seedlings by foliar spray method. Nanotechnology Development. 3: 1-5.
46.Shannon, M.C. 1997. Adaptation of plants to salinity. Advances in Agronomy. 60: 75-120.
47.Shaviv, A. 2000. Advances in controlled release of fertilizers. Advances in Agronomy. 71: 1-49.
48.Shaviv, A. 2005. Controlled Release of Fertilizers. IFA International Workshop on Enhanced-Efficiency Fertilizers,
28-30 Jun. 2005. Frankfurt, Germany.
49.Stevenson, F.J. 1994. Humus Chemistry: Genesis, Composition, Reactions. John Wiley & Sons, New York, 350p.
50.U.S. Salinity Laboratory Staff. 1954. Alkaline-earth carbonates by gravimetric loss of carbon dioxide. P. 105. In: L.A. Richards, (ed.), Diagnosis and improvement of saline and alkali soils. USDA Agric. Handbook. 60. U.S. Government Printing Office, Washington, D.C.
51.U.S. Salinity Laboratory Staff. 1954. pH reading of saturated soil paste. P. 102. In: L.A. Richards, (ed.), Diagnosis and improvement of saline and alkali soils. USDA Agricultural Handbook 60. U.S. Government Printing Office, Washington, D.C.
52.Walin, I., Van, W., Vark, V.J.G., and Vander, L. 1989. Soil and Plant Analysis, a Series of Sylladi. Part 7. Plant Analysis Procedures. Wageningen Agriculture University.
53.Walkley, A., and Black, I.A. 1934. Chromic acid titration for determination of soil organic matter. Soil Science.
63: 251.