اثر سولفات منگنز بر تحمل تنش کم آبی دو رقم انگور در شرایط درون‌شیشه‌ای

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران

2 استاد، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران

3 دانشیار، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

4 دانشیار، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران

5 استادیار، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران

چکیده

خشکی از مهمترین تنش‌های غیرزنده است که روی رشد و جنبه‌های فیزیولوژیکی انگور اثر می‌گذارد. در این پژوهش اثر سولفات منگنز بر صفات مورفو-فیزیولوژیک و فعالیت آنزیم سوپراکسیددیسموتاز دو رقم انگور تامسون‌سیدلس و رطبی تحت تنش کم‌آبی در شرایط درون‌شیشه‌ای ارزیابی شد. آزمایش به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با 8 تکرار انجام شد. از محیط موراشیگ و اسکوگ (MS) با سه تیمار بدون منگنز (صفر میلی‌گرم در لیتر)، 9/16 میلی‌گرم در لیتر (غلظت استاندارد) و 8/33 میلی‌گرم در لیتر (دو برابر غلظت استاندارد) و تیمار تنش کم‌آبی در 4 سطح توسط مقادیر صقر، 3، 9 و 12 درصد وزنی به حجمی پلی‌اتیلن‌گلایکول 6000 برای هر دو رقم انگور استفاده شد. مریستم‌های نوک شاخه به‌عنوان ریز‌نمونه‌های گیاهی استفاده شدند. صقات رشدی شامل ارتفاع گیاه، وزن‌خشک ساقه، سطح و تعداد برگ و محتوای نسبی آب‌برگ تحت تأثیر تنش کم‌آبی کاهش یافت، اما تیمار سولفات منگنز باعث افزایش قابل توجهی در این صفات شد. بیشترین فعالیت آنزیم‌ سوپراکسیددیسموتاز برگ در تیمار 8/33 میلی‌گرم در لیتر سولفات منگنز در سطح تنش 12 درصد پلی‌اتیلن‌گلایکول در رقم رطبی (Umin-1g-1FW 4/65) دیده شد. نتایج این پژوهش پیشنهاد ‌می‌کند که سولفات منگنز می‌تواند موجب افزایش تحمل به تنش کم‌آبی در دو رقم انگور رطبی و تامسون سیدلس در شرایط درون‌شیشه‌ای شود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of MnSO4 on water stress tolerance in two cultivars of grapevine ‎‎(Vitis vinifera cv. L.) under in vitro condition

نویسندگان [English]

  • Parastoo Ghorbani 1
  • Saied Eshghi 2
  • Ahmad Ershadi 3
  • Akhtar Shekafandeh 4
  • Fatemeh Razaghi 5
1 Ph.D. Candidate, Faculty of Agriculture, Shiraz University, Shiraz, Iran
2 Professor, Faculty of Agriculture, Shiraz University, Shiraz, Iran
3 Asociated Professor, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
4 Associate Professor, Faculty of Agriculture, Shiraz University, Shiraz, Iran
5 Assistant Professor, Faculty of Agriculture, Shiraz University, Shiraz, Iran
چکیده [English]

Drought is one of the most important abiotic stresses that have effect on growth and physiological aspect of grape. In this research effect of manganese sulfate on the morpho-physiological traits and superoxide dismutase enzyme activity in Thompson seedless and Rotabi grape cultivars under invitro at drought stress was evaluated. The manganese sulfate treatment was carried out across three levels, including MS without manganese (0 mg/L), MS with a standard manganese concentration (16.9 mg/L) and MS with twice the standard manganese concentration (33.8 mg/L), and the drought stress treatment was performed across four levels using 0, 3, 9 and 12% (w/v) solutions of polyethylene glycol (PEG) 6000 in two grape cultivars, namely seedless Thompson and Rotabi. The meristems of shoottip were used as explants. All growth parameters, including plant height, stem dry weight, leaf area, leaf number and relative water content (RWC) were reduced under the impact of drought. However, manganese sulfate treatment caused a significant increase in all these parameters at all concentrations. The highest amounts of superoxide dismutase enzyme activity was observed in 33.8 mg/L MnSO4 under drought stress with 12% PEG in the Rotabi cultivar (65.4 Umin-1g-1FW). The results of this research suggest that manganese sulfate treatment under invitro condition can improve water stress tolerance in both grapevines seedless Thompson and Rotabi cultivars.

کلیدواژه‌ها [English]

  • Meristems
  • polyethylene glycol
  • plant height
  • SOD‎

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علوم باغبانی ایران
دوره 51، شماره 3
آذر 1399
صفحه 579-588

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سابقه مقاله

  • تاریخ دریافت: 24 اردیبهشت 1398
  • تاریخ بازنگری: 26 مرداد 1398
  • تاریخ پذیرش: 15 شهریور 1398

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