Allergenicity of Bet v 1 in wheat pollen after exposure to drought stress as a model for climate change

Document Type : Research Paper

Authors

1 Ardabil Agricultural and Natural Resources Research and Education Center, Agriculture Research, Education and Extension Organization (AREEO), Ardabil, Iran

2 Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

3 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

Abstract

In the 21st century, climate changes and global warming have become critical concerns, in that they can affect many of the natural phenomena. It is reported that aeroallergen sensitization is influenced by changes in climate. Bet v 1 is an important birch pollen respiratory allergen and a prototype for the PR-10 protein family, which has been reported in wheat pollen. Two wheat genotypes were exposed to water deficit at the meiosis stage of anthers to investigate the effect of drought stress changes on wheat pollen Bet v 1 expression. Then, mature anthers of wheat underwent molecular experiments in the anthesis stage. Expression analysis was carried out, using Real-time PCR, on the Bet v 1 gene that encodes allergens. The results indicated the induction of Bet v 1 in both genotypes in the water deficit condition. The genotype which had been improved by breeders for tolerating drought more than the other genotype revealed more increase in Bet v 1 expression. Given the great spread of wheat, especially improved genotypes worldwide as a pivotal and crop plant and regarding wheat pollen effect on provoking allergy in humans, Bet v 1 can cause new distress in human society. Therefore, this finding is considered as a new verification of concerns of climate change on human health, which emphasizes the importance of efforts to alleviate climate changes to avoid the risk of public health.

Keywords

Article Title [Persian]

آلرژی زایی Bet v 1 در دانه گرده گندم در مواجهه با تنش خشکی، مدلی برای تغییرات اقلیمی

Authors [Persian]

  • نسترن مهری 1
  • رضا فتوت 2
  • احسان محسنی فرد 3
1 مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان اردبیل، سازمان تحقیقات، آموزش و ترویج کشاورزی، اردبیل
2 گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی،دانشگاه زنجان، ایران
3 گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه زنجان، زنجان
Abstract [Persian]

تغییرات اقلیمی و گرمایش جهانی از مشکلات اساسی قرن 21 ام محسوب می‌شود، به نحوی که می‌تواند بسیاری از پدیده‌های طبیعی را تحت تأثیر قرار دهد. گزارش‌هایی وجود دارند مبنی بر این که حساسیت‌زایی آلرژن‌ها تحت تأثیر تغییرات اقلیمی قرار دارند. آلرژن Bet v 1 آلرژن تنفسی مهم دانه گرده درخت غان و پروتوتایپی از خانواده پروتئینی RP-10 است که در دانه گرده گندم گزارش شده است. جهت بررسی اثر تغییرات تنش خشکی روی بیان ژن Bet v 1 در دانه گرده گندم، دو ژنوتیپ گندم در مرحله میوز بساک تحت تأیر تنش خشکی قرار گرفتند. سپس بساک‌های بالغ گندم در مرحله گرده‌افشانی مورد آزمایش‌های مولکولی قرار گرفتند. تجزیه بیان ژن Bet v 1 که کدکننده آلرژن است، با استفاده از دستگاه Real-time PCR انجام گرفت. نتایج نشان‌دهنده القای Real-time PCR در هر دو ژنوتیپ بود. ژنوتیپی که توسط اصلاح‌کنندگان برای تحمل گرما اصلاح شده بود، دارای افزایش بیان بیشتر ژن Bet v 1 بود. به دلیل گسترش گندم، به ویژه گندم‌های اصلاح شده، به عنوان گیاه اساسی و با در نظر گرفتن اثر دانه گرده گندم روی افزایش آلرژی در انسان، ژن Bet v 1 می‌تواند مشکل جدیدی در جامعه بشریت باشد. بنابراین، این یافته تأیید جدیدی است از نگرانی در مورد اثر تغییرات اقلیمی روی سلامت انسان که بر اهمیت تلاش برای کاهش تغییرات اقلیمی جهت پرهیز از به مخاطره انداختن سلامت عمومی تأکید دارد.

Keywords [Persian]

  • آلرژن تنفسی
  • آلرژی دانه گرده درخت غان
  • بیان ژن
  • گرمایش جهانی

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Journal of Plant Physiology and Breeding
Volume 11, Issue 1
June 2021
Pages 87-96

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