Germination and morphophysiological responses of flax (Linum usitatissimum L.) ecotypes to salinity stress

Document Type : Research Paper

Authors

Department of Genetics and Crop Production, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran.

Abstract

Salinity is one of the most important factors that limit plant growth in many regions of the world. In order to evaluate the effects of salinity stress on germination, growth and some physiological characteristics of flax, two separate experiments were carried out in the laboratory and greenhouse. The experimental factors included three ecotypes of flax (Kurdistan Native, L18, E37) and four salinity levels (0, 50, 100 and 150 mM NaCl). The results showed that with increasing salinity, germination percentage, germination rate, seedling vigor index, length and dry weight of hypocotyl and radicle decreased as compared to the control. At 150 mM salinity, Kurdistan Native and E37 ecotypes had the highest and lowest germination and seedling growth, respectively. Also, salinity decreased shoot and root length, shoot and root dry weight and relative water content, whereas, increased electrolytes leakage and soluble sugars. Kurdistan Native had the highest shoot and root dry weight, relative water content and total soluble sugar at 150 mM salinity followed by L18 and E37 ecotypes. At salinity of 150 mM, Kurdistan Native had the lowest electrolyte leakage and L18 and E37 ecotypes had the highest electrolyte leakage. In general, the most sensitive and most tolerant ecotypes were E37 and Kurdistan Native, respectively. Therefore, Kurdistan Native has potential for cultivation in areas with saline water.
 

Keywords

Article Title [Persian]

واکنش جوانه زنی و مورفوفیزیولوژیک اکوتیپ‌های کتان روغنی به تنش شوری

Authors [Persian]

  • بتول مهدوی
  • فرناز علاسوندیاری
Abstract [Persian]

در بسیاری از نواحی جهان شوری از مهمترین فاکتورهای محدود کننده رشد گیاه است. برای ارزیابی اثرات تنش شوری روی جوانه زنی، رشد و برخی ویژگی‌های کتان روغنی دو آزمایش جداگانه در آزمایشگاه و گلخانه انجام شد. فاکتورهای آزمایشی شامل سه اکوتیپ کتان روغنی (بومی کردستان، L18 ، E37) و چهار سطح شوری ( 0، 50، 100 و 150 میلی مولار) بودند. نتایج نشان داد که با افزایش شوری درصد جوانه‌زنی، سرعت جوانه‌زنی، بنیه گیاهچه، طول و وزن خشک ساقه‌چه و ریشه‌چه نسبت به شاهد در هر سه اکوتیپ کاهش یافت. در شوری 150 میلی مولار، اکوتیپ بومی کردستان و E37 به ترتیب بالاترین و پایین­ترین جوانه زنی و رشد گیاهچه را داشتند. همچنین شوری طول ساقه و ریشه، وزن خشک ساقه و ریشه و محتوی نسبی آب را کاهش داد درحالی که نشت الکترولیت‌ها و قندهای محلول را در هر سه اکوتیپ افزایش داد. اکوتیپ بومی کردستان بیشترین وزن خشک ریشه و ساقه، محتوی نسبی آب و قندهای محلول را داشت و بعد از آن L18 و E37 قرار گرفتند. در شوری 150 میلی مولار اکوتیپ بومی کردستان پایین­ترین و اکوتیپ­های L18 وE3 بالاترین نشت الکترولیت­ها را دارا بودند. به طور کلی، حساس‌ترین و متحمل‌ترین اکوتیپ‌ها به ترتیب E37 و بومی کردستان بودند. بنابراین، اکوتیپ بومی کردستان دارای پتانسیل برای کاشت در مناطق با آب شور است.
 
 

Keywords [Persian]

  • پرولین
  • جوانه زنی
  • قندهای محلول کل
  • نشت الکترولیت
  • NaCl

References

Abdul-Baki AA and Anderson JD, 1970. Viability and leaching of sugars from germinating barley. Crop Science 10: 31-33.
Agarie S, Hanaoka N, Kubota F, Agata W and Kaufman B, 1995. Measurement of cell membrane stability evaluated by electrolyte leakage as a drought and heat tolerance test in rice (Oryza sativa L.). Journal of the Faculty of Agriculture, Kyushu University 40(1): 233-240.
Agrawal RL, 2004. Seed Technology. Oxford and IBH Publishing Co., LTD. New Delhi. India, pp. 350.
Ajithkumar PV, Gangadhara KP, Manilal P and Kunhi AAM, 1998. Soil inoculation with Pseudomonas aeruginosa 3 mT eliminates the inhibitory effect of 3-chloroand 4-chlorobenzoate on tomato seed germination. Soil Biology and Biochemistry 30: 1053-1059.
Alasvandyari F, Mahdavi B and Madah Hosseini Sh, 2017. Glycine betaine affects the antioxidant system and ion accumulation and reduces salinity-induced damage in safflower seedlings. Archives of Biological Sciences 69 (1): 139-147.
Ashraf M and Foolad MR, 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany  59(2): 206-216.
Ashraf M and Saghir A, 2000.  "Influence of sodium chloride on ion accumulation, yield components and fibre characteristics in salt-tolerant and salt-sensitive lines of cotton (Gossypium hirsutum L.). Field Crops Research 66(2): 115-127.
Bates LS, Waldren RP and Teare ID, 1973. Rapid determination of free proline for water-studies. Plant and Soil 39: 205-207.
Berti MS, Fischer R, Wilckens F and Hevia Johnson B, 2010. Adaptation and genotype × environment interaction of flaxseed (Linum usitatissimum L.) genotypes in South Central Chile. Chilean Journal of Agricultural Research 70: 345-356.
Bolarian M, Fernandez F, Cruz V and Cuartero J, 1991. Salinity tolerance in four wild tomato species using vegetative yield-salinity response curves. The Journal of American Society of Horticultural Sciences 116: 286–290.
Bordi A, 2010. The influence of salt stress on seed germination, growth and yield of canola cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 38: 128-133.
Dubois M, Gilles KA, Hamilton JK, Reber PA and Smith F, 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28(3): 350-356.
El Goumi Y, Fakiri M,  Lamsaouri O and Benchekroun M, 2014. Salt stress effect on seed germination and some physiological traits in three Moroccan barley (Hordeum vulgare L.) cultivars. Journal of Materials and Environmental Science 5 (2): 625-632.
El-Nagdy GA, Nassar DMA, El-Kady EA and ElYamanee GSA, 2010. Response of flax plant (Linum usitatissimum L.) to treatments with mineral and bio-fertilizers from nitrogen and phosphorus. Journal of American Science 6: 207-217.
Ghoulam C, Foursy A and Fares K, 2002. Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Environmental and Experimental Botany 47: 39-50.
Habtamu A, Ibrahim AH, Eden F and Fikadu T, 2013. Effect of salinity stress on germination and seedling vigour of chickpea (Cicer arietinum L.) cultivars. Academia Journal of Agricultural Research 1(9): 161-166.
Hoagland DR and Arnon DI, 1950. The water culture method for growing plants without soil. California Agricultural Experiment Station, Circular 347. College of Agriculture, University of California, Berkeley, CA, USA.
Housley L and Pollock CJ, 1993. The metabolism of fructan in higher plants. In:Suzuki M and Chatterton NJ (eds). Science and Technology of Fructans. Pp. 191–225. CRC Press, London, UK.
Jabeen N and Ahmad R, 2013. Variations in accession of sunflower and safflower under stress condition. Pakistan Journal of Botany 45(2): 383-389.
Jamil M and Rha ES, 2004. The effect of salinity (NaCl) on the germination and seedling of sugar beet (Beta vulgaris L.) and cabbage (Brassica oleracea L.). Korean Journal of Plant Research 7(3): 226-232.
Katerji N, Van Hoorn JW, Hamdy A, Mastrorilli M and Moukarzel E, 1997. Osmotic adjustment of sugar beets in response to soil salinity and its influence on stomatal conductance, growth and yield. Agricultural Water Management 34: 57-69.
Kaya, C, Higgs D and Kirnak H, 2001. Effects of supplementary phosphorus and potassium on physiological development and mineral nutrition of cucumber and pepper cultivars grown at high salinity (NaCl). Journal of Plant Nutrition 24: 1457-1471.
Lutts S, Kinet JM and Bouharmont J, 1996. NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany 78: 389-398.
Mandhania S, Madan S and Sawhney V, 2006. Antioxidant defense mechanism under salt stress in wheat seedlings. Biologia Plantarum 50: 227-231.
McKersie BD and Leshem YY. 1994. Stress and stress coping in cultivated plants. Kluwer Academic Publishers, London, UK.
Pessarakli M and Szabolcs I, 1999. Soil salinity and sodicity as particular plant/crop stress factors. In: Pessarakli M (ed). Handbook of Plant and Crop Stress, pp. 3–21. CRC Taylor and Francis Group, NY, USA.
Puvanitha S and Mahendran S, 2017. Effect of salinity on plant height, shoot and root dry weight of selected rice cultivars. Scholars Journal of Agriculture and Veterinary Sciences 4(4): 126-131.
Qin J, Dong WY, He KN, Yu Y, Tan GD, Han L, Dong M, Zhang YY, Zhang D, Li AZ and Wang ZL, 2010. NaCl salinity-induced changes in water status, ion contents and photosynthetic properties of Shepherdia argentea (Pursh) Nutt. Seedlings. Plant, Soil and Environment 56: 325–332.
Ritchie SW, Nguyen HT and Haloday AS, 1990. Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Science 30: 105-111.

Saeidi G and Rowland GG, 1999. The effect of temperature, seed colour and linolenic acid concentration on germination and seed vigour in flax. Canadian Journal of Plant Science 79: 315-319.

Sairam RK, Rao KV and Srivastava GC, 2002. Differential response of wheat genotype to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science 163: 1037–1046.

Sharma N, Gupta NK, Gupta S and Hasegawa H, 2005. Effect of NaCl salinity on photosynthetic rate, transpiration rate and oxidative stress tolerance in contrasting wheat genotypes. Photosynthetica 43: 609-613.

Shiri ARM, Safarnejad A and Hamidi H, 2009. Morphological and biochemical characterization of Ferula assafoetida in response to salt stress. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research 17: 38-49 (In Persian with English Abstract).
Taffouo VD, Wamba OF, Youmbi E, Nono GV and Akoa A, 2010.  Growth, yield, water status and ionic distribution response of three bambara groundnut (Vigna subterranea (L.) Verdc.) landraces grown under saline conditions. International Journal of Botany 6(1): 53-58.
Turhan H and Ayaz C, 2004. Effect of salinity on seedling emergence and growth of sunflower (Helianthus annuus L.) cultivars. International Journal of Agriculture and Biology 6(1): 149-152.
Watanabe S, Kojima K, Ide Y and Sasaki S, 1999. Establishment of a tissue culture system of Populus euphratica Oliv. Bulletin of the Tokyo University Forests 102: 87–92.
Zapata PJ, Serrano M, Pretel MS, Amoros A and Botella MA, 2003. Changes in ethylene evolution and polyamine profiles of seedlings of nine cultivars of Lactuca sativa L. in response to salt stress during germination. Plant Science 164: 557-563.
Zhao GQ, Ma BL and Ren CZ, 2007. Growth, gas exchange, chlorophyll fluorescence and ion content of naked oat in response to salinity. Crop Science 47: 123-131.
Journal of Plant Physiology and Breeding
Volume 8, Issue 2
December 2018
Pages 77-87

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