Document Type : Original Article
Authors
- Hooman Shafaee 1
- Fahimeh Farzanegan 1
- Mohammad Sadegh Nazari 2
- Erfan Bardideh 3
- Jalil Tavakkol Afshari 4
- Fatemeh Azani 5
1 Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2 Post Graduated student, Department of Orthodontics, School of Dentistry, Mashhad University of Medical Science, Mashhad. Iran.
3 Department of Orthodontics, School of Dentistry, Mashhad University of Medical Science, Mashhad. Iran
4 Immunology Research Group, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5 Dentist, Private Practice, Mashhad, Iran
Abstract
Background: The aim of study was the biological assessment of cultured RAW264 macrophage exposed to nano amorphous calcium phosphate particles by analyzing of cytotoxicity and genotoxicity tests.
Methods: Nanoamorphus Calcium Phosphate particles were produced by sol-gel method, then particle size and hemogenicity was analyzed by XRD (X ray Diffraction). Cytotoxicity of nanoparticles was determinated with mouse RAW264 macrophage. The cells cultured in 37°c in DMEM medium 96 parts plates with concentration of 10000 cells in each part for 72 hours, then the medium removed and the second medium added to cells containing different concentrations of Nano particle (0, 200 and 400µg/ml). After 24 hours of incubation, MTT assay and Annexin V were used for assessing cell viability and apoptosis.
Results: The apoptosis insignificantly increased in macrophages with 200 and 400µg/ml NACP and for cytotoxicity, cell viability for control, 200µg and 400µg groups were 100,107,103 percent.
Discussion: NACP has no cytotoxicity and genotoxicity, so it can be used as non-toxic and beneficial material for clinical use.
Keywords
Main Subjects
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