تأثیر عصاره پوست گریپ فروت (Citrus paradisi) بر عملکرد رشد و فعالیت آنتی‌اکسیدانی بچه ماهی سفید (Rutilus kutum)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

3 گروه بهداشت و بیماری‌های آبزیان، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران

چکیده

در این مطالعه تأثیر عصاره آبی-الکلی پوست گریپ فروت (Citrus paradisi) بر عملکرد رشد و برخی پارامترهای آنتی ­اکسیدانی پلاسمای خون بچه­ ماهی سفید دریای خزر (Rutilus kutum) مورد مطالعه قرار گرفت. بر این اساس تعداد 180 عدد ماهی با میانگین وزن اولیه 0/3±4/0 گرم به ­مدت 56 روز با رژیم‌های غذایی حاوی میزان 0، 6/25، 12/5 و 25 میلی ‌گرم عصاره آبی-الکلی پوست گریپ فروت در کیلوگرم خوراک تغذیه شدند. بیش ­ترین مقدار افزایش وزن بدن (10/18±50/6 درصد)، نرخ رشد ویژه (0/79±3/05 درصد در روز) و هم­ چنین کم­ ترین مقدار ضریب تبدیل غذایی (32/11±1/0) در ماهیان تغذیه شده با 25 میلی‌ گرم در کیلوگرم خوراک عصاره گریپ فروت مشاهده شد (0/05>p ). در پارامترهای ضریب تبدیل غذایی و درصد بقاء تفاوت معنی‌ داری بین تیمارها دیده نشد (0/05<p ).علاوه بر این، بالاترین میزان فعالیت آنزیم‌ های آنتی ‌اکسیدانی گلوتاتیون پراکسیداز و کاتالاز در تیمار 25 میلی ‌گرم در کیلوگرم GPE مشاهده شد. هم ­چنین سوپراکسید دسموتاز در ماهیان تغذیه شده با عصاره آبی-الکلی پوست گریپ فروت نسبت به گروه شاهد افزایش معنی ‌داری یافت. به ­طورکلی، مکمل­  سازی خوراک بچه ­ماهی سفید با عصاره آبی-الکلی پوست گریپ فروت به ­میزان 25 میلی‌ گرم بر کیلوگرم خوراک، می ‌تواند عملکرد رشد و پاسخ­  های آنتی ­اکسیدانی را بهبود دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Grapefruit (Citrus paradisi) peel extract on growth performance and antioxidant activities of kutum (Rutilus kutum) fry

نویسندگان [English]

  • Zahra Samavat 1
  • Mehdi Shamsaie Mehrgan 1
  • Shahla Jamili 2
  • Mehdi Soltani 3
  • Seyed Pezhman Hosseini Shekarabi 1
1 Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Iranian Fisheries Research Organization, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
3 Department of Aquatic Animal Health, Veterinary Faculty, University of Tehran, Tehran, Iran
چکیده [English]

In this study, the effects of the hydro-alcoholic extract of grapefruit peel (Citrus paradisi) on growth performance and some antioxidant parameters of Caspian white fish (Rutilus kutum) blood plasma were investigated. Accordingly, 180 fry fish with an initial mean weight of 4.0 ± 0.3 g were fed with diets containing 0, 6.25, 12.5 and 25 mg hydro-alcoholic extract of grapefruit peel per kg feed. The fry fish were cultured for 56 days. The highest amounts of body weight gain (50.10±6.18 %), specific growth rate (3.05±0.79 % per day) as well as the lowest feed conversion ratio (1.32±0.11) were observed in the fish fed with Grapefruit juice extract 25 mg/kg hydro-alcoholic extract of grapefruit peel (p < 0.05). There were no significant differences between treatments in condition factor and survival percentage (P>0.05). In addition, the highest activities of antioxidant enzymes including glutathione peroxidase enzyme and catalase enzyme were observed in 25 mg/kg hydro-alcoholic extract of grapefruit peel. Also, superoxide dismutase enzyme was significantly increased in fish fed with hydro-alcoholic extract of grapefruit peel compared to the control group. In general, supplementation of the Caspian white fish feed with hydro alcoholic extract of grapefruit peel at 25 mg/kg of feed can improve growth performance and antioxidant responses.

کلیدواژه‌ها [English]

  • Antioxidant activity
  • Growth
  • Caspian white fish
  • Grapefruit
  • Herbal extract
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