بررسی تاثیر سم خالص سازی شده میکروسیستین (Microcystin-RR) از ریز جلبک (Microcystis aeruginosa) بر زنده مانی دافنی ماگنا ((1820 ,Daphnia magna (Straus) و تعیین غلظت کشنده آن (LC50-96h)

نوع مقاله : محیط زیست جانوری

نویسندگان

گروه شیلات، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

این تحقیق جهت بررسی تاثیر سم خالص سازی شده MC-RR) Microcystin-RR) از ریز جلبک Microcystis aeroginosa بر زنده مانی دافنی ماگنا (Daphnia magna) و تعیین غلظت کشنده این سم (LC50-96) انجام شد. برای انجام این آزمایش تعداد 21 عدد دافنی ماگنا در هر تکرار، در معرض غلظت های 0/5، 2، 10، 20 و 50 میکروگرم بر میلی لیتر سم MC-RR قرار گرفتند و میزان بقای دافنی ­ها برای مدت 24 تا 96 ساعت مورد محاسبه قرار گرفت. هم چنین یک تیمار به عنوان تیمار شاهد (بدون سم) در نظر گرفته شد. هر تیمار 3 تکرار داشت. با توجه به نتایج این تحقیق، سم میکروسیستین (MC-RR) تاثیر کشنده بر دافنی ماگنا داشت به طوری که حتی در کم ترین غلظت (غلظت 0/5) حدود 38 درصد تلفات مشاهده گردید و هیچ یک از دافنی ماگناها توانایی زنده ماندن در غلظت 2 میکرو گرم بر میلی لیتر سم خالص MC-RR به مدت 96 ساعت را نداشتند. قابل ذکر است توانایی زنده ماندن دافنی­ ها با افزایش غلظت سم، کاهش پیدا کرد، به طوری که در غلظت 10 میکرو گرم بر میلی لیتر سم، در روز دوم و در غلظت 20 و 50 میکرو گرم بر میلی لیتر سم، در روز اول تمامی دافنی ­ها از بین رفتند. میزان LC50-96 ،0/58 میکرو گرم بر میلی لیتر سم MC-RR به دست آمد. نتایج این تحقیق نشان داد که در غلظت 1/75 میکرو گرم بر میلی لیتر MC-RR، در مدت 96 ساعت، 99 درصد دافنی ­ها می ­میرند.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of pure microcystin (microcystin-RR) from Microcystis aeroginosa (Kützing, 1846) on the survival of Daphnia magna (Straus, 1820) and determination of its lethal concentration (LC50-96h)

نویسندگان [English]

  • Saeed Balali
  • Seyed Abbas Hoseini
  • Rasoul Ghorbani
Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
چکیده [English]

This study was carried out to investigate the effect of Microcystin-RR (MC-RR) extracted from Microcystis aeroginosa microalgae on Daphnia magna and determine the lethal concentration of this toxin (LC50-96h). Twenty-one Daphnia magna were exposed to concentrations of 0.5, 2, 10, 20 and 50 μg.ml-1 of MC-RR Microcystin in each replicate, and the daphnia survival for 24 to 96 hours was calculated. One treatment was considered as control (no toxin), each treatment had 3 replications. According to the results of this study, microcystin (MC-RR) had a lethal effect on Daphnia magna, so that even at a low concentration (concentration of 0.5), about 38% of mortality was observed, and none of the Daphnia magna had the ability to survive at 2 μg.ml-1 of pure MC-RR Microcystin for 96 hours. This ability decreased by increasing the concentration of poison, so that at the concentration of 10 μg.ml-1 of Microcystin, on the second day and at concentrations of 20 and 50 μg.ml-1 of poison, all daphnia were dead on the first day. The results of this study showed that in the concentration of 1.75 μg.ml-1 MC-RR Microcystin, during 96 hours, 99 percent of daphnia were died.

کلیدواژه‌ها [English]

  • Microcystin
  • Microcystis aeroginosa
  • Survival
  • Daphnia magna
  • LC50-96h

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فصلنامه محیط زیست جانوری
دوره 12، شماره 1
فروردین 1399
صفحه 361-368

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