Response of Osteoblasts and Osteoclasts in Regenerating Scales to Gravity Loading

Nobuo Suzuki; Kei-Ichiro Kitamura; Katsunori Omori; Tetsu Nemoto; Yusuke Satoh; Makoto J. Tabata; Mika Ikegame; Toshio Yamamoto; Kenichi Ijiri; Yukihiro Furusawa; Takashi Kondo; Ichiro Takasaki; Yoshiaki Tabuchi; Shigehito Wada; Nobuaki Shimizu; Yuichi Sasayama; Masato Endo; Toshio Takeuchi; Masayuki Nara; Masanori Somei; Yusuke Maruyama; Kazuichi Hayakawa; Toru Shimazu; Yuko Shigeto; Sachiko Yano; Atsuhiko Hattori

doi:10.2187/bss.23.211

Nobuo Suzuki, Kei-Ichiro Kitamura, Katsunori Omori, Tetsu Nemoto, Yusuke Satoh, Makoto J. Tabata, Mika Ikegame, Toshio Yamamoto, Kenichi Ijiri, Yukihiro Furusawa, Takashi Kondo, Ichiro Takasaki, Yoshiaki Tabuchi, Shigehito Wada, Nobuaki Shimizu, Yuichi Sasayama, Masato Endo, Toshio Takeuchi, Masayuki Nara, Masanori Somei, Yusuke Maruyama, Kazuichi Hayakawa, Toru Shimazu, Yuko Shigeto, Sachiko Yano, Atsuhiko Hattori

Author information

Nobuo Suzuki
Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Housu-gun, Ishikawa 927-0553, Japan
Kei-Ichiro Kitamura
Department of Clinical Laboratory Science, Division of Health Sciences, Graduate School of Medical Science, Kanazawa University, Kodatsuno, Ishikawa 920-0942, Japan
Katsunori Omori
Japan Aerospace Exploration Agency, Tsukuba, Ibaraki 305-8505, Japan
Tetsu Nemoto
Department of Clinical Laboratory Science, Division of Health Sciences, Graduate School of Medical Science, Kanazawa University, Kodatsuno, Ishikawa 920-0942, Japan
Yusuke Satoh
Department of Laboratory Sciences, School of Health Sciences, Kanazawa University, Kodatsuno, Ishikawa 920-0942, Japan
Makoto J. Tabata
Biostructural Science, Department of Hard Tissue Engineering, Division of Bio-Matrix, Graduate School of Tokyo Medical and Dental University, Bukyo-ku, Tokyo 113-8549, Japan
Mika Ikegame
Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Okayama 700-8525, Japan
Toshio Yamamoto
Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Okayama 700-8525, Japan
Kenichi Ijiri
Radioisotope Center, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan
Yukihiro Furusawa
Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Toyama 930-0194, Japan
Takashi Kondo
Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Toyama 930-0194, Japan
Ichiro Takasaki
Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Sugitani, Toyama 930-0194, Japan
Yoshiaki Tabuchi
Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Sugitani, Toyama 930-0194, Japan
Shigehito Wada
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of Toyama, Sugitani, Toyama 930-0194, Japan
Nobuaki Shimizu
Institute of Nature and Environmental Technology, Kanazawa University, Kakuma, Ishikawa 920-1192, Japan
Yuichi Sasayama
Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Housu-gun, Ishikawa 927-0553, Japan
Masato Endo
Department of Marine Biosciences, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Konan, Minato-ku, Tokyo 108-8477, Japan
Toshio Takeuchi
Department of Marine Biosciences, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Konan, Minato-ku, Tokyo 108-8477, Japan
Masayuki Nara
Department of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Chiba 272-0827, Japan
Masanori Somei
Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Ishikawa 920-1192, Japan
Yusuke Maruyama
Department of Biology, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Chiba 272-0827, Japan
Kazuichi Hayakawa
Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma, Ishikawa 920-1192, Japan
Toru Shimazu
Japan Space Forum, Department of Science and Applications, Chiyoda-ku, Tokyo 100-0004, Japan
Yuko Shigeto
Japan Aerospace Exploration Agency, Tsukuba, Ibaraki 305-8505, Japan
Sachiko Yano
Japan Aerospace Exploration Agency, Tsukuba, Ibaraki 305-8505, Japan
Atsuhiko Hattori
Department of Biology, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Chiba 272-0827, Japan

JOURNAL FREE ACCESS

2009 Volume 23 Issue 4 Pages 211-217

DOI https://doi.org/10.2187/bss.23.211

Details

Abstract

It is known that the teleost scale regenerates after being removed. We previously reported that the osteogenesis in regenerating scales was very similar to that in calvarial bone, which suggests that regenerating scale can be used as a model for osteogenesis to analyze the interaction between osteoblasts and osteoclasts. In the present study, we developed an assay system using regenerating scales by modifying our assay system with normal scales. The weight of one regenerating scale cannot be measured precisely because the rate of calcification in regenerating scales is lower than that in normal scales. In regenerating scales, thus, the respective marker enzyme activity, i.e., alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts, was normalized by the surface area (mm²) of each goldfish scale. With this modified method, we were able to detect ALP and TRAP activities with no variation in the lines of regenerating scales. In addition, we found that the ALP activity in the regenerating scales significantly increased under 3G acceleration loading by vibration, while the TRAP activity in the loaded regenerating scales significantly decreased. We strongly believe that the regenerating scale is a good material for the analysis of bone metabolism under different gravity environments.

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