Optimization of Low-Cost Medium Composition for the Production of α-Amylase by Bacillus amyloliquefaciens Using a Semi-Solid Substrate

Title:Optimization of Low-Cost Medium Composition for the Production of α-Amylase by Bacillus amyloliquefaciens Using a Semi-Solid Substrate

Volume: 7 Issue: 4

Author(s): Veyis Selen*M. Saban Tanyildizi

Affiliation:

• Department of Chemical Engineering, Firat University, 23279 Elazig,Turkey

Keywords: α-Amylase, semi-solid substrate fermentation, low-cost medium, response surface methodology, fermentation, culture broths.

Abstract: Aims: A low-cost medium for α-amylase is critical for the growing demands of industries.

Method: In this study, α-amylase production was investigated using different agro-industrial products (wheat bran, cheese whey, soy bean cake, hazelnut oil cake) and supplemental nutrients (peptone, ammonium sulfate) using semi-solid substrate fermentation (SSSF).

Observation: The experiments were planned in two stages. The first aim was to screen important nutrients for enzyme production using the Placket-Burman design.

Result: Three components (wheat bran, cheese whey, and peptone) were determined to have an important effect on α-amylase production. The possibility of further optimization was examined by using the Central Composite Design.

Conclusion: The maximum α-amylase activity was obtained (11,196 U/ml) using 4.9 g/l of wheat bran, 28.7 v/v% cheese whey, and 3.7 g/l of peptone.

Cite this article as:

Selen Veyis *, Tanyildizi Saban M. , Optimization of Low-Cost Medium Composition for the Production of α-Amylase by Bacillus amyloliquefaciens Using a Semi-Solid Substrate, Current Physical Chemistry 2017; 7 (4) . https://dx.doi.org/10.2174/1877946807666170808123636