Molecular Evaluation of Exon 8 Cystathionine rs5742905T T>C Gene Polymorphism and Determination of its Frequency, Distribution Pattern, and Association with Susceptibility to Coronary Artery Disease in the North Indian Population

Title:Molecular Evaluation of Exon 8 Cystathionine rs5742905T T>C Gene Polymorphism and Determination of its Frequency, Distribution Pattern, and Association with Susceptibility to Coronary Artery Disease in the North Indian Population

Volume: 21 Issue: 2

Author(s): Mamta P. Sumi*, Sameer A. Guru, Rashid Mir, Samantak Sahu, Musadiq A. Bhat, M.P. Girish and Alpana Saxena

Affiliation:

• Department of Biochemistry, Maulana Azad Medical College, University of Delhi, Delhi,India

Keywords: Coronary artery disease (CAD), cystathionine β synthase (CBS), PCR-RFLP technique, homocystinuria, homozygous mutant (CC), heterozygous (TC).

Abstract:

Background: The protein coded by the cystathionine β synthase (CBS) gene acts as a catalyzer and converts homocysteine to cystathionine. Impairment of the CBS gene leads to homocystinuria by cystathionine β synthase deficiency which is linked to Coronary Artery Disease. A number of polymorphisms studies have been performed on the cystathionine β synthase gene. In the current study, we planned to analyze the influence of CBS T833C gene polymorphism(exon 8 cystathionine rs5742905T T>C), its association with Coronary Artery Disease development, and its progression in the north Indian population.

Materials and Methods: The present study comprises 100 angiographically confirmed CAD patients and 100 age and sex-matched healthy controls. A total of 50% or more luminal stenosis at one major coronary artery was considered for the inclusion criteria of the cases. The investigation of T833C polymorphism in the CBS gene was performed by PCR- RFLP technique.

Results: As a result, we found that homozygous mutant (CC) and heterozygous (TC) genotypes of CBS T833C gene polymorphism were significantly higher in CAD patients than in healthy subjects. We also observed a substantially increased CAD risk in dominant, codominant inheritance, and allele-specific models for the CBS T833C gene polymorphism. We analyzed the differential distribution with respect to disease severity, but there was no significant association (p=0.96).

Conclusion: In conclusion, this study demonstrates that CBS T833C gene polymorphism plays a key role in developing coronary artery disease and its progression.

Cite this article as:

Sumi P. Mamta *, Guru A. Sameer , Mir Rashid, Sahu Samantak , Bhat A. Musadiq , Girish M.P. and Saxena Alpana , Molecular Evaluation of Exon 8 Cystathionine rs5742905T T>C Gene Polymorphism and Determination of its Frequency, Distribution Pattern, and Association with Susceptibility to Coronary Artery Disease in the North Indian Population, Cardiovascular & Hematological Disorders-Drug Targets 2021; 21 (2) . https://dx.doi.org/10.2174/1871529X21666210315121027