Cell Cycle Related Differentiation of Bone Marrow Cells into Lung Cells

Dooner, Mark; Aliotta, Jason M; Pimental, Jeffrey; Dooner, Gerri J; Abedi, Mehrdad; Colvin, Gerald; Liu, Qin; Weier, Heinz-Ulli; Dooner, Mark S; Quesenberry, Peter J

doi:10.2172/936517

Title: Cell Cycle Related Differentiation of Bone Marrow Cells into Lung Cells

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Abstract

Green-fluorescent protein (GFP) labeled marrow cells transplanted into lethally irradiated mice can be detected in the lungs of transplanted mice and have been shown to express lung specific proteins while lacking the expression of hematopoietic markers. We have studied marrow cells induced to transit cell cycle by exposure to IL-3, IL-6, IL-11 and steel factor at different times of culture corresponding to different phases of cell cycle. We have found that marrow cells at the G1/S interface have a 3-fold increase in cells which assume a lung phenotype and that this increase is no longer seen in late S/G2. These cells have been characterized as GFP{sup +} CD45{sup -} and GFP{sup +} cytokeratin{sup +}. Thus marrow cells with the capacity to convert into cells with a lung phenotype after transplantation show a reversible increase with cytokine induced cell cycle transit. Previous studies have shown the phenotype of bone marrow stem cells fluctuates reversibly as these cells traverse cell cycle, leading to a continuum model of stem cell regulation. The present studies indicate that marrow stem cell production of nonhematopoietic cells also fluctuates on a continuum.

Authors:: Dooner, Mark; Aliotta, Jason M; Pimental, Jeffrey; Dooner, Gerri J; Abedi, Mehrdad; Colvin, Gerald; Liu, Qin; Weier, Heinz-Ulli; Dooner, Mark S; Quesenberry, Peter J

Publication Date:: Mon Dec 31 00:00:00 EST 2007

Research Org.:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: Life Sciences Division

OSTI Identifier:: 936517

Report Number(s):: LBNL-842E
TRN: US200818%%855

DOE Contract Number:: DE-AC02-05CH11231

Resource Type:: Technical Report

Country of Publication:: United States

Language:: English

Subject:: 60; BONE MARROW; BONE MARROW CELLS; CAPACITY; CELL CYCLE; LUNGS; LYMPHOKINES; MICE; PHENOTYPE; PRODUCTION; PROTEINS; RESPIRATORY TRACT CELLS; STEELS; STEM CELLS

Citation Formats


                    Dooner, Mark, Aliotta, Jason M, Pimental, Jeffrey, Dooner, Gerri J, Abedi, Mehrdad, Colvin, Gerald, Liu, Qin, Weier, Heinz-Ulli, Dooner, Mark S, and Quesenberry, Peter J. Cell Cycle Related Differentiation of Bone Marrow Cells into Lung Cells.  United States: N. p., 2007. 
        Web.  doi:10.2172/936517.

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                    Dooner, Mark, Aliotta, Jason M, Pimental, Jeffrey, Dooner, Gerri J, Abedi, Mehrdad, Colvin, Gerald, Liu, Qin, Weier, Heinz-Ulli, Dooner, Mark S, & Quesenberry, Peter J. Cell Cycle Related Differentiation of Bone Marrow Cells into Lung Cells.  United States.  https://doi.org/10.2172/936517

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                    Dooner, Mark, Aliotta, Jason M, Pimental, Jeffrey, Dooner, Gerri J, Abedi, Mehrdad, Colvin, Gerald, Liu, Qin, Weier, Heinz-Ulli, Dooner, Mark S, and Quesenberry, Peter J. 2007.  
        "Cell Cycle Related Differentiation of Bone Marrow Cells into Lung Cells".  United States.  https://doi.org/10.2172/936517.  https://www.osti.gov/servlets/purl/936517.

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@article{osti_936517,

  title        = {Cell Cycle Related Differentiation of Bone Marrow Cells into Lung Cells},

  author       = {Dooner, Mark and Aliotta, Jason M and Pimental, Jeffrey and Dooner, Gerri J and Abedi, Mehrdad and Colvin, Gerald and Liu, Qin and Weier, Heinz-Ulli and Dooner, Mark S and Quesenberry, Peter J},

  abstractNote = {Green-fluorescent protein (GFP) labeled marrow cells transplanted into lethally irradiated mice can be detected in the lungs of transplanted mice and have been shown to express lung specific proteins while lacking the expression of hematopoietic markers. We have studied marrow cells induced to transit cell cycle by exposure to IL-3, IL-6, IL-11 and steel factor at different times of culture corresponding to different phases of cell cycle. We have found that marrow cells at the G1/S interface have a 3-fold increase in cells which assume a lung phenotype and that this increase is no longer seen in late S/G2. These cells have been characterized as GFP{sup +} CD45{sup -} and GFP{sup +} cytokeratin{sup +}. Thus marrow cells with the capacity to convert into cells with a lung phenotype after transplantation show a reversible increase with cytokine induced cell cycle transit. Previous studies have shown the phenotype of bone marrow stem cells fluctuates reversibly as these cells traverse cell cycle, leading to a continuum model of stem cell regulation. The present studies indicate that marrow stem cell production of nonhematopoietic cells also fluctuates on a continuum.},

  doi          = {10.2172/936517},

  url          = {https://www.osti.gov/biblio/936517},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Mon Dec 31 00:00:00 EST 2007},

  month        = {Mon Dec 31 00:00:00 EST 2007}

}

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