Characterizing the Catalytic Potential of Deinococcus, Arthrobacter and other Robust Bacteria in Contaminated Subsurface Environments of the Hanford Site
Until recently, there have been no clear physiologic predictors of a cell's ability to recover from ionizing radiation (IR), desiccation, and other DOE-relevant oxidative stress conditions. In general, the most resistant bacteria have been Gram-positive (e.g., Deinococcus, Arthrobacter, Lactobacillus & Enterococcus spp.) and the most sensitive have been Gram-negative (e.g., Pseudomonas, Shewanella & Neisseria spp.). However, there are several reported exceptions to this paradigm, the Gram-negative cyanobacterium Chroococcidiopsis is extremely resistant to IR, whereas the Gram-positive Micrococcus luteus is sensitive. We have identified biomolecular signatures for radiation sensitivity and resistance which are independent of phylogeny, where very high and very low intracellular Mn/Fe concentration ratios correlated with very high and very low resistances, respectively; and restricting Mn(II) in the famously resistant Deinococcus radiodurans sensitized this eubacterium to IR (http://cfyn.ifas.ufl.edu/radiation.pdf).
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Henry M. Jackson Foundation, Uniformed Services University of the Health Sciences
- Sponsoring Organization:
- USDOE Office of Science (SC)
- OSTI ID:
- 895911
- Report Number(s):
- ERSD-1024843-2006; R&D Project: ERSD 1024843; TRN: US0700554
- Country of Publication:
- United States
- Language:
- English
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Accumulation of Mn(II) in Deinococcus radiodurans Facilitates Gamma-Radiation Resistance
Characterizing the Catalytic Potential of Deinococcus, Arthrobacter and other Robust Bacteria in Contaminated Subsurface Environments of the Hanford Site