Development of a Vaccine for Bacterial Kidney Disease in Salmon, 1986 Annual Report.
Bacterial kidney disease (BRD) has been and remains a chronic contributory problem limiting the productivity of salmon of the Columbia River Basin. Control of this disease will not come easily, but it would lead to a tremendous increase in the health and numbers of salmon populations. Vaccination of salmon of Renibacterium salmoninarum (KDB) is a potentially successful method of controlling this disease. To date, however, no successful vaccine has been developed for general use. A possible solution to this problem,and thus the goal of this research, is to isolate the antigenic components of KDB and enhance their ability to activate the host defenses. This will be accomplished by the chemical modification of these antigens with potent immunomodulatory substances. These modified antigens will then be tested for their effectiveness in inducing immunity to BKD and thereby preventing the disease. The goal of the project's third year was to test the immunogenicity and prophylactic value in coho salmon (Oncorhynchus kisutch) of various chemical conjugates of Renibacterium salmoninarum cells and major antigens. This was accomplished by assessing the serum antibody response, the cellular immune response (cellular proliferation), and the kinetics of mortality after Lethal injections of the bacterium. An important facet of this research is the identification and isolation of virulence factors. These studies are not only important to the dissection of the mechanism of pathogenesis of bacterial kidney disease, but the purification of such a factor(s) will insure the production of a more potent vaccine. The studies completed this year have: (1) identified antigenic material which protect; (2) identified antigenic material which can exacerbate the disease; (3) identified a possibly major mechanism of pathogenesis via the interference with antibody; (4) the general ability to produce delineated a western blot technique for identification of infected fish; (5) described the use of monoclonal antibodies for antigenic analysis; and (6) identified an unusual and dramatic effect of R. salmoninarum cells on phagocytic function.
- Research Organization:
- Oregon State University.
- Sponsoring Organization:
- United States. Bonneville Power Administration.
- DOE Contract Number:
- 1984BP16480
- OSTI ID:
- 760069
- Report Number(s):
- DOE/BP-16480-3; R&D Project: 1984-046-00; TRN: AH200027%%68
- Resource Relation:
- Other Information: PBD: 1 Jun 1987
- Country of Publication:
- United States
- Language:
- English
Similar Records
Development of a Vaccine for Bacterial Kidney Disease in Salmon, 1987 Annual Report.
Development of a Vaccine for Bacterial Kidney Disease in Salmon, 1988 Final Report.