Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements
Abstract
Experiments examined the effects of radiation dose-rate and protein synthesis inhibition expression of cytoskeletal and matrix elements in Syrian hamster embryo cells. Results demonstrated little effect of dose-rate for neutrons when comparing expression of {alpha}-tubulin and fibronectin genes. Cycloheximide repressed accumulation of {alpha}-tubulin-mRNA following exposure to high dose-rate neutrons or {gamma} rays. Cycloheximide did not affect accumulation of actin mRNA. Cycloheximide abrogated induction of fibronectin-mRNA which occurred following exposure to {gamma} rays and high dose-rate neutrons. These results suggest a role for labile proteins in the maintenance of {alpha}-tubulin and fibronectin mRNA accumulation following exposure to radiation. 24 refs., 3 tabs.
- Authors:
-
- Argonne National Lab., IL (United States)
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE, Washington, DC (United States)
- OSTI Identifier:
- 70715
- Report Number(s):
- ANL/CMB/PP-84857
ON: DE95012218; TRN: 95:004783
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Technical Report
- Resource Relation:
- Other Information: PBD: [1995]
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; PROTEINS; BIOLOGICAL RADIATION EFFECTS; SYNTHESIS; IONIZING RADIATIONS; GAMMA RADIATION; RADIATION DOSES; DOSE RATES; GENES; ANIMAL CELLS; CYCLOHEXIMIDE; MESSENGER-RNA; GENETIC EFFECTS; DNA-CLONING
Citation Formats
Woloschak, G E, Loyola Univ. Medical Center, Maywood, IL, Felcher, P, and Chang-Liu, Chin-Mei. Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements. United States: N. p., 1995.
Web. doi:10.2172/70715.
Woloschak, G E, Loyola Univ. Medical Center, Maywood, IL, Felcher, P, & Chang-Liu, Chin-Mei. Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements. United States. https://doi.org/10.2172/70715
Woloschak, G E, Loyola Univ. Medical Center, Maywood, IL, Felcher, P, and Chang-Liu, Chin-Mei. 1995.
"Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements". United States. https://doi.org/10.2172/70715. https://www.osti.gov/servlets/purl/70715.
@article{osti_70715,
title = {Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements},
author = {Woloschak, G E and Loyola Univ. Medical Center, Maywood, IL and Felcher, P and Chang-Liu, Chin-Mei},
abstractNote = {Experiments examined the effects of radiation dose-rate and protein synthesis inhibition expression of cytoskeletal and matrix elements in Syrian hamster embryo cells. Results demonstrated little effect of dose-rate for neutrons when comparing expression of {alpha}-tubulin and fibronectin genes. Cycloheximide repressed accumulation of {alpha}-tubulin-mRNA following exposure to high dose-rate neutrons or {gamma} rays. Cycloheximide did not affect accumulation of actin mRNA. Cycloheximide abrogated induction of fibronectin-mRNA which occurred following exposure to {gamma} rays and high dose-rate neutrons. These results suggest a role for labile proteins in the maintenance of {alpha}-tubulin and fibronectin mRNA accumulation following exposure to radiation. 24 refs., 3 tabs.},
doi = {10.2172/70715},
url = {https://www.osti.gov/biblio/70715},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jun 01 00:00:00 EDT 1995},
month = {Thu Jun 01 00:00:00 EDT 1995}
}