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Title: Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements

Abstract

Experiments examined the effects of radiation dose-rate and protein synthesis inhibition expression of cytoskeletal and matrix elements in Syrian hamster embryo cells. Results demonstrated little effect of dose-rate for neutrons when comparing expression of {alpha}-tubulin and fibronectin genes. Cycloheximide repressed accumulation of {alpha}-tubulin-mRNA following exposure to high dose-rate neutrons or {gamma} rays. Cycloheximide did not affect accumulation of actin mRNA. Cycloheximide abrogated induction of fibronectin-mRNA which occurred following exposure to {gamma} rays and high dose-rate neutrons. These results suggest a role for labile proteins in the maintenance of {alpha}-tubulin and fibronectin mRNA accumulation following exposure to radiation. 24 refs., 3 tabs.

Authors:
 [1]; ;  [1]
  1. Argonne National Lab., IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
70715
Report Number(s):
ANL/CMB/PP-84857
ON: DE95012218; TRN: 95:004783
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1995]
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; PROTEINS; BIOLOGICAL RADIATION EFFECTS; SYNTHESIS; IONIZING RADIATIONS; GAMMA RADIATION; RADIATION DOSES; DOSE RATES; GENES; ANIMAL CELLS; CYCLOHEXIMIDE; MESSENGER-RNA; GENETIC EFFECTS; DNA-CLONING

Citation Formats

Woloschak, G E, Loyola Univ. Medical Center, Maywood, IL, Felcher, P, and Chang-Liu, Chin-Mei. Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements. United States: N. p., 1995. Web. doi:10.2172/70715.
Woloschak, G E, Loyola Univ. Medical Center, Maywood, IL, Felcher, P, & Chang-Liu, Chin-Mei. Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements. United States. https://doi.org/10.2172/70715
Woloschak, G E, Loyola Univ. Medical Center, Maywood, IL, Felcher, P, and Chang-Liu, Chin-Mei. 1995. "Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements". United States. https://doi.org/10.2172/70715. https://www.osti.gov/servlets/purl/70715.
@article{osti_70715,
title = {Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: Dose-rate effects and protein synthesis requirements},
author = {Woloschak, G E and Loyola Univ. Medical Center, Maywood, IL and Felcher, P and Chang-Liu, Chin-Mei},
abstractNote = {Experiments examined the effects of radiation dose-rate and protein synthesis inhibition expression of cytoskeletal and matrix elements in Syrian hamster embryo cells. Results demonstrated little effect of dose-rate for neutrons when comparing expression of {alpha}-tubulin and fibronectin genes. Cycloheximide repressed accumulation of {alpha}-tubulin-mRNA following exposure to high dose-rate neutrons or {gamma} rays. Cycloheximide did not affect accumulation of actin mRNA. Cycloheximide abrogated induction of fibronectin-mRNA which occurred following exposure to {gamma} rays and high dose-rate neutrons. These results suggest a role for labile proteins in the maintenance of {alpha}-tubulin and fibronectin mRNA accumulation following exposure to radiation. 24 refs., 3 tabs.},
doi = {10.2172/70715},
url = {https://www.osti.gov/biblio/70715}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jun 01 00:00:00 EDT 1995},
month = {Thu Jun 01 00:00:00 EDT 1995}
}