''Aged'' (dense) circulating red cells contain normal concentrations of ATP

Kirkpatrick, F. H.; Muhs, A. G.; Kostuk, R. K.; Gabel, C. W.

doi:10.2172/6230591

Title: ''Aged'' (dense) circulating red cells contain normal concentrations of ATP

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Abstract

A newly-developed technique for determination of the ATP content of individual red cells to the densest, and hence presumably the oldest, cells from normal human blood was applied. It was found that these cells contain normal concentrations of ATP, although the net content of ATP is decreased. The essence of the technique is suspension of red cells in autologous plasma containing luciferin and luciferase, lysis of the cells with a pulse from a laser, and counting of the photoemissions resulting from reaction of the released ATP with the luciferase. These data appear to disprove the otherwise plausible hypothesis of Lichtman that red cells decline exponentially in ATP content as they age, by one of the suggested tests of this hypothesis. The data suggest an alternative hypothesis: red cells maintain an approximately constant concentration of ATP as they age, and red cell destruction is caused by factors other than cellular ATP.

Authors:: Kirkpatrick, F. H.; Muhs, A. G.; Kostuk, R. K.; Gabel, C. W.

Publication Date:: Sun Jan 01 00:00:00 EST 1978

Research Org.:: Univ. of Rochester, NY (United States)

OSTI Identifier:: 6230591

Report Number(s):: UR-3490-1543

DOE Contract Number:: EY-76-C-02-3490

Resource Type:: Technical Report

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; ATP; BIOCHEMISTRY; CHEMICAL ANALYSIS; ERYTHROCYTES; ABUNDANCE; AGE DEPENDENCE; BLOOD; ECOLOGICAL CONCENTRATION; LASERS; LUCIFERASE; LUCIFERIN; LYSIS; ALBUMINS; BIOLOGICAL MATERIALS; BLOOD CELLS; BODY FLUIDS; CHEMISTRY; ENZYMES; NUCLEOTIDES; ORGANIC COMPOUNDS; OXIDASES; OXIDOREDUCTASES; PROTEINS; 550200* - Biochemistry

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                    Kirkpatrick, F. H., Muhs, A. G., Kostuk, R. K., and Gabel, C. W. ''Aged'' (dense) circulating red cells contain normal concentrations of ATP.  United States: N. p., 1978. 
        Web.  doi:10.2172/6230591.

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                    Kirkpatrick, F. H., Muhs, A. G., Kostuk, R. K., & Gabel, C. W. ''Aged'' (dense) circulating red cells contain normal concentrations of ATP.  United States.  https://doi.org/10.2172/6230591

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                    Kirkpatrick, F. H., Muhs, A. G., Kostuk, R. K., and Gabel, C. W. 1978.  
        "''Aged'' (dense) circulating red cells contain normal concentrations of ATP".  United States.  https://doi.org/10.2172/6230591.  https://www.osti.gov/servlets/purl/6230591.

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@article{osti_6230591,

  title        = {''Aged'' (dense) circulating red cells contain normal concentrations of ATP},

  author       = {Kirkpatrick, F. H. and Muhs, A. G. and Kostuk, R. K. and Gabel, C. W.},

  abstractNote = {A newly-developed technique for determination of the ATP content of individual red cells to the densest, and hence presumably the oldest, cells from normal human blood was applied. It was found that these cells contain normal concentrations of ATP, although the net content of ATP is decreased. The essence of the technique is suspension of red cells in autologous plasma containing luciferin and luciferase, lysis of the cells with a pulse from a laser, and counting of the photoemissions resulting from reaction of the released ATP with the luciferase. These data appear to disprove the otherwise plausible hypothesis of Lichtman that red cells decline exponentially in ATP content as they age, by one of the suggested tests of this hypothesis. The data suggest an alternative hypothesis: red cells maintain an approximately constant concentration of ATP as they age, and red cell destruction is caused by factors other than cellular ATP.},

  doi          = {10.2172/6230591},

  url          = {https://www.osti.gov/biblio/6230591},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Sun Jan 01 00:00:00 EST 1978},

  month        = {Sun Jan 01 00:00:00 EST 1978}

}

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