Study of the radiation effects on nucleic acids and related compounds. Progress report, August 15, 1976--November 14, 1977
The effects of ionizing radiation on nucleic acids and components were studied in vitro. Our approach involves four levels of operation and progress is being made at each stage. First, procedures were established to separate and purify three reactive radiation products from thymidine. Second, improved methods of synthesizing trans-glycols of pyrimidines were developed, and a new method for the stereospecific synthesis of cis-glycol of pyrimidines was realized. Thirdly, the Ames Salmonella test was used to determine the mutagenicity of the radiation products and the reactive ones from thymine and thymidine were found to be highly mutagenic. Therefore, all radiation products should be considered potential human health hazards and should be screened when they can be purified and synthesized. In the fourth stage, the reaction of each nucleic-acid base with Cu/sup + +/ and cis-5,6-dihydro-6-hydroperoxy-5-hydroxythymine (6-TOOH) was studied in order to further our understanding of the molecular mechanisms of radiation mutagenesis. The presence of Cu/sup + +/ was shown to be necessary for the effective mutagenic activity of 6-TOOH in the H. influenzae transformation assay. These findings provide fundamental information about the possible health hazards of ionizing radiation and will be useful in designing methods to protect against and repair radiation damage, which may be mutagenic and carcinogenic.
- Research Organization:
- Johns Hopkins Univ., Baltimore, Md. (USA)
- DOE Contract Number:
- EY-76-S-02-3286
- OSTI ID:
- 5227378
- Report Number(s):
- COO-3286-17; TRN: 78-004438
- Country of Publication:
- United States
- Language:
- English
Similar Records
Study of the radiation effects on nucleic acids and related compounds. Annual progress report, August 15, 1974--August 14, 1975. [X radiation, hamster cells, Haemophilus influenzae]
Synthesis of dihydrothymidine and thymidine glycol 5'-triphosphates and their ability to serve as substrates for Escherichia coli DNA polymerase I
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
NUCLEIC ACIDS
CHEMICAL RADIATION EFFECTS
IN VITRO
THYMIDINE
BIOSYNTHESIS
RADIOLYSIS
THYMINE
MUTAGENESIS
BIOASSAY
BIOCHEMICAL REACTION KINETICS
CHEMICAL COMPOSITION
COPPER COMPOUNDS
GLYCOLS
HYDROXY COMPOUNDS
MOLECULAR BIOLOGY
PYRIMIDINES
SEPARATION PROCESSES
SYNERGISM
ALCOHOLS
AZINES
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
HETEROCYCLIC COMPOUNDS
KINETICS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATION CHEMISTRY
RADIATION EFFECTS
REACTION KINETICS
RIBOSIDES
SYNTHESIS
TRANSITION ELEMENT COMPOUNDS
URACILS
560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
550200 - Biochemistry