نوع المستند : المقالة الأصلية
المؤلفون
1
Theriogenology Department, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, Egypt.
2
Immunopharmacology Unit, Department of Artificial Insemination and embryo transfer, Animal Reproduction Research Institute, Cairo, Egypt.
3
Department of Artificial Insemination and embryo transfer, Animal Reproduction Research Institute, Cairo, Egypt.
المستخلص
The present work aimed to study the effect of cryopreservation on individual motility, mitochondrial activity, plasma membrane, acrosome, and DNA integrities of bull spermatozoa in response to the addition of different concentrations of bovine serum albumin (BSA) to frozen semen extender. Four adult fertile healthy bulls were enrolled in this study, semen samples were collected weekly from each bull for six successive weeks using an artificial vagina (of 42°C temperature). After semen evaluation, ejaculates from each bull were diluted in a Tris-based buffer egg yolk extender and cooled to 5°C. The Semen extender was supplemented by five different concentrations of BSA;0 (as a control group), 0.5, 1, 1.5, and 2 g/100 ml. Samples were processed for cryopreservation. Each cooled ejaculate was split into five equal aliquots and diluted with BSA supplemented extender; then semen was packaged in 0.25 ml straws and placed 4 cm above liquid nitrogen before being plunged into a liquid nitrogen tank and stored. Frozen straws were thawed in a water bath at 37°C for 30 sec and then evaluated for assessment of individual motility, mitochondrial activity, and plasma membrane and acrosome integrities. The results of the present study showed that post-thaw sperm motility and integrity of plasma membrane were significantly better (P<0.05) in semen samples containing 1.0% BSA (60.00±2.88 and 59.67±1.45 respectively) than in control sample (52.33±1.45 and 53.67±1.85, respectively). Moreover, BSA 1%-supplemented group had better motility and plasma membrane integrity than the other three groups; supplemented with BSA 0.5%-, BSA 1.5%-, and BSA 2%. Also, acrosome integrity of spermatozoa was significantly better (P<0.05) in the semen sample containing 1.0% BSA (62.33±2.60) than in the control one (57.00±1.53) and the other three groups; BSA 0.5%-, BSA 1.5%-, and BSA 2%-supplemented groups. Increasing the concentration of BSA above 1% appeared to have a deleterious effect on sperm DNA expressed as an increase in the percentage of fragmented DNA, DNA content in the tail of the comet, tail length, and Olive tail moment. These results suggested a protective role of BSA 1% supplementation in bull frozen semen extender that is, in turn, reflected in the improvement of individual motility, mitochondrial activity, and plasma membrane, acrosome, and DNA integrities after the freezing-thawing cycle.
الكلمات الرئيسية
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